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[173610_at] Msi3p

Description CEK005A7F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C30C11.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27009 /WB_GENE_ID=C30C11.4 /WP=CE00103 /CHR=3 /FEA=Genomic Cluster /DEF=Msi3p; C.elegans cDNA clone yk5a7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 47      6grp_total > 49      4grp_total > 01      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3520
1 2 3 4 range
A 4504.7
(-0.3)
P 		2911.7
(-3)
P 		4700.7
(0)
P 		6431.9
(3)
P 		3520
B 3583
(-2)
P 		3160.9
(-3)
P 		3360.1
(-2.5)
P 		5777.3
(3)
P 		2616
C 4042.8
(-1.8)
P 		3602.7
(-3)
P 		4278.1
(-1.1)
P 		5787.3
(3)
P 		2185
avr 4043.5
(-1.2) 		3225.1
(-3) 		4113
(-1.1) 		5998.8
(3) 		2774

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3316
1 2 3 4 5 6 range
A 4877.2
(3)
P 		3211.1
(-1)
P 		2943.7
(-1.6)
P 		2377.7
(-3)
P 		4150.9
(1.3)
P 		4217.4
(1.4)
P 		2500
B 4328.5
(3)
P 		3738.4
(1.7)
P 		1560.8
(-3)
P 		3330.2
(0.8)
P 		3386.7
(1)
P 		4324.5
(3)
P 		2768
C 3148.7
(-1.7)
P 		4034.5
(1.8)
P 		2870.7
(-2.8)
P 		3014.3
(-2.2)
P 		2816.1
(-3)
P 		4343.1
(3)
P 		1527
avr 4118.1
(2.4) 		3661.3
(0.9) 		2458.4
(-3) 		2907.4
(-1.5) 		3451.2
(0.2) 		4295
(3) 		1837
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1008
1 2 3 4 range
A 1640.2
(3)
P 		632
(-3)
P 		1228.9
(0.6)
P 		941.5
(-1.2)
P 		1008
B 1142.6
(1.8)
P 		780.7
(-3)
P 		778.6
(-3)
P 		1231.1
(3)
P 		453
C 1338.3
(1.2)
P 		1035.8
(-2.3)
P 		1491
(3)
P 		975.8
(-3)
P 		515
avr 1373.7
(3) 		816.2
(-3) 		1166.2
(0.8) 		1049.5
(-0.5) 		558

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1234
1 2 3 4 5 6 range
A 899.1
(-0.3)
P 		1142
(2)
P 		641.1
(-2.6)
P 		603
(-3)
P 		1252.6
(3)
P 		1026.8
(0.9)
P 		650
B 1506.1
(2.5)
P 		1244.3
(1.2)
P 		379
(-3)
P 		882.1
(-0.6)
P 		1376
(1.8)
P 		1612.6
(3)
P 		1234
C 1167.1
(1)
P 		1231
(1.4)
P 		730.6
(-2.1)
P 		606.2
(-3)
P 		911.3
(-0.8)
P 		1453.9
(3)
P 		848
avr 1190.8
(1.7) 		1205.8
(1.8) 		583.6
(-3) 		697.1
(-2.1) 		1180
(1.6) 		1364.4
(3) 		781
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173610_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23876
Sequence Type Exemplar sequence
Representative Public ID CEK005A7F NCBI
Target Description CEK005A7F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C30C11.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27009 /WB_GENE_ID=C30C11.4 /WP=CE00103 /CHR=3 /FEA=Genomic Cluster /DEF=Msi3p; C.elegans cDNA clone yk5a7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066467(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066467 NCBI Caenorhabditis elegans C30C11.4 (Msi3p) mRNA, complete cds. 11/11 None
C30C11.4 ENSEMBL cdna:known chromosome:CEL140:III:8443820:8446827:-1 gene:C30C11.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8443837-8446798(-) 98.61 100.0
Public Domain and Genome References
Gene Title Msi3p
Ensembl C30C11.4
Entrez Gene 176195 Entrez gene
SwissProt Q05036 EMBL-EBI
WormBase CE00103 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.19944.1.S1_S_AT similar to Heat shock 70 kDa protein 4L (Osmotic stress protein 94) (Heat shock 70-related protein APG-1) cfa
CANINE_2:CFAAFFX.6801.1.S1_AT similar to Heat shock 70 kDa protein 4L (Osmotic stress protein 94) (Heat shock 70-related protein APG-1) cfa
CHICKEN:GGAAFFX.6405.1.S1_AT heat shock 70kDa protein 4-like gga
CHICKEN:GGAAFFX.6405.2.S1_S_AT heat shock 70kDa protein 4-like gga
HG-U133_PLUS_2:205543_AT heat shock 70kDa protein 4-like hs
HG-U133A:205543_AT heat shock 70kDa protein 4-like hs
HG-FOCUS:205543_AT heat shock 70kDa protein 4-like hs
HG-U133A_2:205543_AT heat shock 70kDa protein 4-like hs
HU35KSUBD:RC_AA398923_AT heat shock 70kDa protein 4-like hs
U133_X3P:G7656893_3P_AT heat shock 70kDa protein 4-like hs
HG-U95AV2:40354_AT heat shock 70kDa protein 4-like hs
HG-U95D:78874_AT heat shock 70kDa protein 4-like hs
HU35KSUBC:RC_AA478479_AT Heat shock 70kDa protein 4-like hs
MOUSE430_2:1418253_A_AT heat shock 70kDa protein 4 like mm
MOE430A:1418253_A_AT heat shock 70kDa protein 4 like mm
MOUSE430A_2:1418253_A_AT heat shock 70kDa protein 4 like mm
MU11KSUBA:U23921_S_AT heat shock 70kDa protein 4 like mm
MG-U74AV2:99489_AT heat shock 70kDa protein 4 like mm
MG-U74BV2:114595_AT heat shock 70kDa protein 4 like mm
MG-U74CV2:165748_AT heat shock 70kDa protein 4 like mm
MOE430A:1449010_AT heat shock 70kDa protein 4 like mm
MOUSE430A_2:1449010_AT heat shock 70kDa protein 4 like mm
MOUSE430_2:1449010_AT heat shock 70kDa protein 4 like mm
MOE430B:1458385_AT heat shock 70kDa protein 4 like mm
MOUSE430_2:1458385_AT heat shock 70kDa protein 4 like mm
RAE230B:1378002_AT osmotic stress protein 94 kDa (predicted) rn
RAT230_2:1378002_AT osmotic stress protein 94 kDa (predicted) rn
RAE230A:1375626_AT Osmotic stress protein 94 kDa (predicted) rn
RAT230_2:1375626_AT Osmotic stress protein 94 kDa (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA27967 Hypothetical protein C30C11.4 [Caenorhabditis elegans] ref|NP_498868.1| C30C11.4 [Caenorhabditis elegans] sp|Q05036|YLA4_CAEEL Hypothetical protein C30C11.4 in chromosome III 0.0
blast CAE71253 Hypothetical protein CBG18133 [Caenorhabditis briggsae] 0.0
blast XP_623199 PREDICTED: similar to ENSANGP00000015293 [Apis mellifera] 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173610_AT
gtcgattagatctctacattttctatcacttttcgtatcaaaggattatctcaatgtttg
aatgaatccctattttttctcttttcttacaatccgttttcgtgatccctagtgtgtgtt
ttcattgtgttctcgattgttaaaccctcgtagtaagatctcgaatcgagcacttttgta
ttcgcccaatctgcgtcttcctcgcatactagaaagttttatgttccggttgtttttctt
tttttttattattttaaataattattatatttattgccctcccaattcgtcatgtcttat
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGATTAGATCTCTACATTTTCTA 506 473 2336 Antisense
ATTTTCTATCACTTTTCGTATCAAA 169 15 2353 Antisense
GTATCAAAGGATTATCTCAATGTTT 61 455 2370 Antisense
TGAATCCCTATTTTTTCTCTTTTCT 37 577 2398 Antisense
TCTTTTCTTACAATCCGTTTTCGTG 111 615 2415 Antisense
TTACAATCCGTTTTCGTGATCCCTA 81 683 2422 Antisense
TTCGTGATCCCTAGTGTGTGTTTTC 364 695 2434 Antisense
TTGTGTTCTCGATTGTTAAACCCTC 535 703 2460 Antisense
TCTCGATTGTTAAACCCTCGTAGTA 520 609 2466 Antisense
CTGCGTCTTCCTCGCATACTAGAAA 594 235 2526 Antisense
CCTCCCAATTCGTCATGTCTTATGA 487 267 2613 Antisense
Sequence Source Affymetrix Proprietary Database