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[173595_s_at]

Description C65820 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y32H12A.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65820 /WB_GENE_ID=Y32H12A.8 /WP=CE28516 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk405c5 : 5prime end, single read.
Cluster 6grp_high1_10_10 > 36      4grp_high_8_8 > 10      6grp_total > 36      4grp_total > 14      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 74.8
(3)
P 		54.1
(-1.3)
A 		46.1
(-3)
A 		63.5
(0.6)
P 		29
B 78.5
(-0.8)
P 		51.8
(-2.4)
A 		41.6
(-3)
A 		140.4
(3)
P 		99
C 98.8
(3)
P 		43.2
(-2.4)
P 		36.8
(-3)
P 		81.9
(1.4)
P 		62
avr 84
(1.7) 		49.7
(-2.1) 		41.5
(-3) 		95.3
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 99.5
(3)
P 		98.4
(2.9)
P 		51.3
(-3)
A 		58.4
(-2.1)
P 		51.3
(-3)
P 		53.5
(-2.7)
P 		48
B 84.8
(2.5)
P 		78.1
(1.6)
P 		65.5
(-0.1)
P 		60.1
(-0.9)
P 		44.4
(-3)
P 		88.5
(3)
P 		44
C 92.2
(3)
P 		82
(1.7)
P 		44.4
(-2.9)
A 		65.4
(-0.3)
P 		43.8
(-3)
P 		72.4
(0.5)
P 		48
avr 92.2
(3) 		86.2
(2.2) 		53.7
(-2.1) 		61.3
(-1.1) 		46.5
(-3) 		71.5
(0.3) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 27.2
(3)
P 		11.8
(-2.2)
A 		12
(-2.1)
A 		9.3
(-3)
P 		18
B 25
(1.6)
P 		12.8
(-2.1)
A 		9.6
(-3)
A 		29.9
(3)
P 		20
C 32.7
(3)
P 		12.4
(-3)
P 		12.8
(-2.9)
P 		13.8
(-2.6)
P 		20
avr 28.3
(3) 		12.3
(-2.7) 		11.5
(-3) 		17.7
(-0.8) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 18.3
(-1.2)
P 		35
(3)
P 		11.2
(-3)
A 		14.8
(-2.1)
P 		15.5
(-1.9)
P 		13
(-2.5)
P 		24
B 29.5
(1.8)
P 		26
(0.5)
P 		15.9
(-3)
P 		15.9
(-3)
P 		18
(-2.3)
P 		33
(3)
P 		17
C 34.2
(3)
P 		25
(0.6)
P 		11.3
(-3)
A 		13.1
(-2.5)
P 		14.2
(-2.2)
P 		24.2
(0.4)
P 		23
avr 27.3
(2.5) 		28.7
(3) 		12.8
(-3) 		14.6
(-2.3) 		15.9
(-1.8) 		23.4
(1) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173595_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24304
Sequence Type Exemplar sequence
Representative Public ID C65820 NCBI
Target Description C65820 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y32H12A.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65820 /WB_GENE_ID=Y32H12A.8 /WP=CE28516 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk405c5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065750 NCBI Caenorhabditis elegans Y32H12A.8 (Y32H12A.8) mRNA, complete cds. 11/11 A 
Y32H12A.8 ENSEMBL cdna:known chromosome:CEL140:III:5385482:5404941:-1 gene:Y32H12A.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5385491-5404938(-) 98.11 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32H12A.8
Entrez Gene 175742 Entrez gene
SwissProt Q9N533 EMBL-EBI
WormBase CE28516 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18851.1.S1_S_AT similar to WD repeat domain 24 cfa
CANINE_2:CFAAFFX.30004.1.S1_S_AT similar to WD repeat domain 24 cfa
DROSOPHILA_2:1640643_A_AT dm
DROSGENOME1:154050_AT dm
CHICKEN:GGAAFFX.11378.1.S1_AT WD repeat domain 24 gga
CHICKEN:GGAAFFX.1637.1.S1_S_AT WD repeat domain 24 gga
HG-U133B:223293_AT WD repeat domain 24 hs
HG-U133_PLUS_2:223293_AT WD repeat domain 24 hs
HU35KSUBA:RC_AA403312_S_AT WD repeat domain 24 hs
HU35KSUBD:RC_AA302632_AT WD repeat domain 24 hs
U133_X3P:G12053230_3P_AT WD repeat domain 24 hs
HG-U95B:48491_AT WD repeat domain 24 hs
HG-U95E:68512_AT WD repeat domain 24 hs
HG-U95D:72188_AT WD repeat domain 24 hs
MOE430B:1455212_AT WD repeat domain 24 mm
MOUSE430_2:1455212_AT WD repeat domain 24 mm
MG-U74CV2:165771_R_AT WD repeat domain 24, mRNA (cDNA clone MGC:47001 IMAGE:5148210) mm
RG-U34C:RC_AI073006_R_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI179411_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI073006_S_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAE230A:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAT230_2:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60491 Hypothetical protein Y32H12A.8 [Caenorhabditis elegans] ref|NP_498151.1| Y32H12A.8 [Caenorhabditis elegans] 0.0
blast CAE61332 Hypothetical protein CBG05170 [Caenorhabditis briggsae] 0.0
blast XP_397394 PREDICTED: similar to ENSANGP00000004131 [Apis mellifera] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.01
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.6E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.22
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0048
Sequence
Target Sequence 
>C. ELEGANS:173595_S_AT
acgagatgttggaaggaattgcggatcttgacaaatttaagattgaggcgaagcttccgg
caaaaaagaaagatttattgaaacatatcaatgaattaattgttgaaaattctgatacag
cacactggtcatggggtgatttgaaaatgcagacgagaatacgaaagattccaccagcaa
atagaatgaagccgaagcatattaatcgtattttgaagggaaagaaaatgtacaaaccgc
cgaaaaaatacgatccaaaagatgcgcaaactaacggacgcaagtggttctag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAGATGTTGGAAGGAATTGCGGA 603 93 11379 Antisense
GGAATTGCGGATCTTGACAAATTTA 683 531 11393 Antisense
AAGATTGAGGCGAAGCTTCCGGCAA 60 157 11417 Antisense
GAGGCGAAGCTTCCGGCAAAAAAGA 374 403 11423 Antisense
AATTCTGATACAGCACACTGGTCAT 199 177 11486 Antisense
AGCACACTGGTCATGGGGTGATTTG 493 75 11497 Antisense
AATACGAAAGATTCCACCAGCAAAT 262 173 11536 Antisense
CCACCAGCAAATAGAATGAAGCCGA 488 273 11549 Antisense
GAAGCCGAAGCATATTAATCGTATT 548 343 11566 Antisense
ATACGATCCAAAAGATGCGCAAACT 480 23 11626 Antisense
AACTAACGGACGCAAGTGGTTCTAG 458 121 11647 Antisense
Sequence Source Affymetrix Proprietary Database