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[173594_at]

Description C65820 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y32H12A.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65820 /WB_GENE_ID=Y32H12A.8 /WP=CE28516 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk405c5 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 27      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 80      6grp_total > 27      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 254.5
(3)
P 		116
(-3)
P 		162.3
(-1)
P 		116
(-3)
P 		139
B 256.6
(2.3)
P 		125.2
(-1.7)
P 		83.5
(-3)
P 		279.7
(3)
P 		196
C 343.7
(3)
P 		158.3
(-2.8)
P 		152.6
(-3)
P 		201.8
(-1.5)
P 		191
avr 284.9
(3) 		133.2
(-3) 		132.8
(-3) 		199.2
(-0.4) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 240.8
(3)
P 		240
(2.9)
P 		147.8
(-3)
P 		149.2
(-2.9)
P 		154.8
(-2.5)
P 		190.2
(-0.3)
P 		93
B 308.5
(3)
P 		236.6
(0.5)
P 		194.3
(-0.9)
P 		170.1
(-1.8)
P 		134.7
(-3)
P 		240.8
(0.7)
P 		174
C 384.2
(3)
P 		237.5
(-1.1)
P 		206.2
(-2)
P 		208
(-1.9)
P 		169.2
(-3)
P 		234.2
(-1.2)
P 		215
avr 311.2
(3) 		238
(0.2) 		182.8
(-1.9) 		175.8
(-2.1) 		152.9
(-3) 		221.7
(-0.4) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 92.7
(3)
P 		25.2
(-2.4)
P 		42.4
(-1)
P 		17
(-3)
P 		76
B 81.8
(3)
P 		30.9
(-1.9)
P 		19.4
(-3)
P 		59.6
(0.9)
P 		62
C 113.8
(3)
P 		45.5
(-2.1)
P 		53.2
(-1.6)
P 		34
(-3)
P 		80
avr 96.1
(3) 		33.9
(-3) 		38.3
(-2.6) 		36.9
(-2.7) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 44.4
(-1.6)
P 		85.3
(3)
P 		32.2
(-3)
P 		37.9
(-2.4)
P 		46.7
(-1.4)
P 		46.3
(-1.4)
P 		53
B 107.4
(3)
P 		78.7
(0.2)
P 		47.2
(-2.8)
P 		45.1
(-3)
P 		54.7
(-2.1)
P 		89.8
(1.3)
P 		62
C 142.4
(3)
P 		72.5
(-1.2)
P 		52.5
(-2.4)
P 		41.8
(-3)
P 		54.7
(-2.2)
P 		78.4
(-0.8)
P 		101
avr 98.1
(3) 		78.8
(1) 		44
(-2.7) 		41.6
(-3) 		52
(-1.9) 		71.5
(0.2) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173594_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24304
Sequence Type Exemplar sequence
Representative Public ID C65820 NCBI
Target Description C65820 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y32H12A.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65820 /WB_GENE_ID=Y32H12A.8 /WP=CE28516 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk405c5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065750 NCBI Caenorhabditis elegans Y32H12A.8 (Y32H12A.8) mRNA, complete cds. 11/11 None
Y32H12A.8 ENSEMBL cdna:known chromosome:CEL140:III:5385482:5404941:-1 gene:Y32H12A.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5385491-5404938(-) 98.11 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32H12A.8
Entrez Gene 175742 Entrez gene
SwissProt Q9N533 EMBL-EBI
WormBase CE28516 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18851.1.S1_S_AT similar to WD repeat domain 24 cfa
CANINE_2:CFAAFFX.30004.1.S1_S_AT similar to WD repeat domain 24 cfa
DROSOPHILA_2:1640643_A_AT dm
DROSGENOME1:154050_AT dm
CHICKEN:GGAAFFX.11378.1.S1_AT WD repeat domain 24 gga
CHICKEN:GGAAFFX.1637.1.S1_S_AT WD repeat domain 24 gga
HG-U133B:223293_AT WD repeat domain 24 hs
HG-U133_PLUS_2:223293_AT WD repeat domain 24 hs
HU35KSUBA:RC_AA403312_S_AT WD repeat domain 24 hs
HU35KSUBD:RC_AA302632_AT WD repeat domain 24 hs
U133_X3P:G12053230_3P_AT WD repeat domain 24 hs
HG-U95B:48491_AT WD repeat domain 24 hs
HG-U95E:68512_AT WD repeat domain 24 hs
HG-U95D:72188_AT WD repeat domain 24 hs
MOE430B:1455212_AT WD repeat domain 24 mm
MOUSE430_2:1455212_AT WD repeat domain 24 mm
MG-U74CV2:165771_R_AT WD repeat domain 24, mRNA (cDNA clone MGC:47001 IMAGE:5148210) mm
RG-U34C:RC_AI073006_R_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI179411_AT similar to Hypothetical protein MGC47001 (predicted) rn
RG-U34C:RC_AI073006_S_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAE230A:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
RAT230_2:1373938_AT similar to Hypothetical protein MGC47001 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60491 Hypothetical protein Y32H12A.8 [Caenorhabditis elegans] ref|NP_498151.1| Y32H12A.8 [Caenorhabditis elegans] 0.0
blast CAE61332 Hypothetical protein CBG05170 [Caenorhabditis briggsae] 0.0
blast XP_397394 PREDICTED: similar to ENSANGP00000004131 [Apis mellifera] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.01
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.6E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.22
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0048
Sequence
Target Sequence 
>C. ELEGANS:173594_AT
gcaagtggttctaggaaggatatacagttttcttcttgttttatatatatattttttgtt
aaattttttgttatctttatctcatttttatagccatttttccaatgtatttcgtattcc
cctcaattgttctcccattatttttttcaaaattttgaaattctttcaatcgctagtgat
tataaggtatattttcaatatgtgataattttgtatatatatatttgaatcgatttgcgc
ccatcaaattgcctttctatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAGTGGTTCTAGGAAGGATATAC 204 321 11658 Antisense
AAGGATATACAGTTTTCTTCTTGTT 156 161 11673 Antisense
ATATACAGTTTTCTTCTTGTTTTAT 157 19 11677 Antisense
GTTATCTTTATCTCATTTTTATAGC 512 447 11727 Antisense
ATCTCATTTTTATAGCCATTTTTCC 510 31 11736 Antisense
TAGCCATTTTTCCAATGTATTTCGT 705 649 11748 Antisense
TTTCCAATGTATTTCGTATTCCCCT 643 667 11756 Antisense
CCTCAATTGTTCTCCCATTATTTTT 700 267 11778 Antisense
TGAAATTCTTTCAATCGCTAGTGAT 699 571 11813 Antisense
TTTGAATCGATTTGCGCCCATCAAA 583 667 11881 Antisense
GCCCATCAAATTGCCTTTCTATTTT 122 287 11896 Antisense
Sequence Source Affymetrix Proprietary Database