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[173592_s_at]

Description C49849 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=H21P03.3A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C49849 /WB_GENE_ID=H21P03.3A /WP=CE19930 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk474g3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 97      6grp_total > 88      4grp_total > 01      6grp_highest_sub > Da      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1473
1 2 3 4 range
A 1012.8
(-1.5)
P 		790.8
(-3)
P 		1080.9
(-1.1)
P 		1691.3
(3)
P 		901
B 863.7
(-2.8)
P 		822.7
(-3)
P 		890.4
(-2.7)
P 		2263.5
(3)
P 		1441
C 887.8
(-3)
P 		981.4
(-2.4)
P 		1082.8
(-1.7)
P 		1791
(3)
P 		903
avr 921.4
(-2.7) 		865
(-3) 		1018
(-2.1) 		1915.3
(3) 		1050

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1068
1 2 3 4 5 6 range
A 886.3
(-1.8)
P 		831.5
(-2.2)
P 		693.4
(-3)
P 		823.4
(-2.2)
P 		1287.9
(0.6)
P 		1673.9
(3)
P 		981
B 997
(-0.8)
P 		929.8
(-1.2)
P 		607.6
(-3)
P 		1285.1
(0.8)
P 		1120.1
(-0.1)
P 		1675.3
(3)
P 		1068
C 848.3
(-2)
P 		778.7
(-2.6)
P 		727.5
(-3)
P 		783.7
(-2.5)
P 		1274.3
(1.5)
P 		1455.6
(3)
P 		728
avr 910.5
(-1.5) 		846.7
(-1.9) 		676.2
(-3) 		964.1
(-1.1) 		1227.4
(0.6) 		1601.6
(3) 		925
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 368.8
(3)
P 		171.7
(-3)
P 		282.6
(0.4)
P 		247.6
(-0.7)
P 		197
B 275.4
(-1.4)
P 		203.2
(-3)
P 		206.3
(-2.9)
P 		482.4
(3)
P 		279
C 293.9
(-2.3)
P 		282.2
(-3)
P 		377.4
(3)
P 		302
(-1.8)
P 		95
avr 312.7
(1.5) 		219
(-3) 		288.8
(0.4) 		344
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 477
1 2 3 4 5 6 range
A 163.4
(-2.7)
P 		295.7
(0.4)
P 		151
(-3)
P 		208.8
(-1.6)
P 		388.6
(2.6)
P 		407.5
(3)
P 		257
B 346.9
(-0.5)
P 		309.5
(-1)
P 		147.6
(-3)
P 		340.4
(-0.6)
P 		455.1
(0.9)
P 		624.7
(3)
P 		477
C 314.4
(-0.1)
P 		237.6
(-1.5)
P 		185.2
(-2.5)
P 		157.6
(-3)
P 		412.3
(1.6)
P 		487.3
(3)
P 		330
avr 274.9
(-1) 		280.9
(-0.9) 		161.3
(-3) 		235.6
(-1.7) 		418.7
(1.5) 		506.5
(3) 		345
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173592_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24846
Sequence Type Exemplar sequence
Representative Public ID C49849 NCBI
Target Description C49849 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=H21P03.3A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C49849 /WB_GENE_ID=H21P03.3A /WP=CE19930 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk474g3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069768(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069768 NCBI Caenorhabditis elegans SphingoMyelin Synthase family member (sms-1) (sms-1) mRNA, complete cds. 11/11 None
H21P03.3a ENSEMBL cdna:known chromosome:CEL140:IV:11482693:11487965:-1 gene:H21P03.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178072 2 1 NM_069768 173592_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11482709-11487885(-) 100.0 100.0
Public Domain and Genome References
Ensembl H21P03.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB09114 Hypothetical protein H21P03.3a [Caenorhabditis elegans] ref|NP_502169.1| SphingoMyelin Synthase family member (sms-1) [Caenorhabditis elegans] 0.0
blast CAB54269 Hypothetical protein H21P03.3b [Caenorhabditis elegans] ref|NP_502168.1| SphingoMyelin Synthase family member (sms-1) [Caenorhabditis elegans] sp|Q9U3D4|SMS1_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 1 (Sphingomyelin synthase 1) 0.0
blast CAE62109 Hypothetical protein CBG06147 [Caenorhabditis briggsae] 1.0E-162
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502169.1 6 146-168,188-210,217-239,263-280,285-307,317-339
H21P03.3a 6 146-168,188-210,217-239,263-280,285-307,317-339
Sequence
Target Sequence 
>C. ELEGANS:173592_S_AT
tctctgccaaaatagcacactcctgaatcgacattaaaaactgctccgccgttactttta
tgggtttcgccacgaattttcttgcctttcaaacggctcctttaccttctgcgttttgat
tgcttattgtaaccccttttttttaaattttcaatttttcccaacccatcccacatcgat
ttcgtcgtctttgaaactacgggtaactcttttcccactcattctttgtcaattgcgtag
tatttttgttgaacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCTGCCAAAATAGCACACTCCTG 591 611 1352 Antisense
ATAGCACACTCCTGAATCGACATTA 106 23 1363 Antisense
TCGACATTAAAAACTGCTCCGCCGT 663 601 1379 Antisense
TCTTGCCTTTCAAACGGCTCCTTTA 120 619 1431 Antisense
CCTTTACCTTCTGCGTTTTGATTGC 660 267 1450 Antisense
ACCTTCTGCGTTTTGATTGCTTATT 468 87 1455 Antisense
TGATTGCTTATTGTAACCCCTTTTT 493 563 1468 Antisense
CCCACATCGATTTCGTCGTCTTTGA 578 257 1521 Antisense
GATTTCGTCGTCTTTGAAACTACGG 633 425 1529 Antisense
TCCCACTCATTCTTTGTCAATTGCG 693 597 1564 Antisense
ATTGCGTAGTATTTTTGTTGAACAT 477 5 1583 Antisense
Sequence Source Affymetrix Proprietary Database