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[173590_at]

Description CEK012B7R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F10E7.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27258 /WB_GENE_ID=F10E7.8 /WP=CE04363 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk12b7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 87      6grp_total > 69      4grp_total > 54      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1073
1 2 3 4 range
A 1411.8
(3)
P 		1158.7
(1.4)
P 		908.8
(-0.3)
P 		488.8
(-3)
P 		923
B 1561.8
(3)
P 		974.5
(-0.8)
P 		767.8
(-2.1)
P 		629.6
(-3)
P 		932
C 1453.5
(3)
P 		776.9
(-1.6)
P 		1143
(0.9)
P 		572
(-3)
P 		882
avr 1475.7
(3) 		970
(-0.3) 		939.9
(-0.5) 		563.5
(-3) 		912

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 703
1 2 3 4 5 6 range
A 1283.9
(0.8)
P 		1375.4
(1.6)
P 		836.1
(-3)
P 		1002.3
(-1.6)
P 		1457.2
(2.3)
P 		1539.5
(3)
P 		703
B 1215.6
(-0.6)
P 		1268.8
(0.1)
P 		1018.7
(-3)
P 		1167
(-1.2)
P 		1133.3
(-1.6)
P 		1501.6
(3)
P 		483
C 1199.3
(-0.3)
P 		1031.8
(-3)
P 		1218.9
(0)
P 		1399.9
(3)
P 		1044.3
(-2.8)
P 		1255.9
(0.7)
P 		368
avr 1232.9
(0.1) 		1225.3
(-0) 		1024.6
(-3) 		1189.7
(-0.6) 		1211.6
(-0.2) 		1432.3
(3) 		408
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 443
1 2 3 4 range
A 514
(3)
P 		251.5
(-0.6)
P 		237.6
(-0.7)
P 		71.5
(-3)
P 		443
B 498
(3)
P 		240.7
(-1.2)
P 		177.9
(-2.3)
P 		134.2
(-3)
P 		364
C 481.1
(3)
P 		223.4
(-1)
P 		398.4
(1.7)
P 		96.5
(-3)
P 		385
avr 497.7
(3) 		238.5
(-0.9) 		271.3
(-0.4) 		100.7
(-3) 		397

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 378
1 2 3 4 5 6 range
A 236.7
(-1.9)
P 		489.2
(3)
P 		182.1
(-3)
P 		254.2
(-1.6)
P 		439.7
(2)
P 		374.8
(0.8)
P 		307
B 423
(0.4)
P 		422.3
(0.4)
P 		247.4
(-3)
P 		309.1
(-1.8)
P 		460.5
(1.1)
P 		559.9
(3)
P 		313
C 444.5
(3)
P 		314.8
(-1.8)
P 		310.2
(-1.9)
P 		281.5
(-3)
P 		337.9
(-0.9)
P 		420.4
(2.1)
P 		163
avr 368.1
(0.6) 		408.8
(1.7) 		246.6
(-3) 		281.6
(-2) 		412.7
(1.9) 		451.7
(3) 		205
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173590_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23526
Sequence Type Exemplar sequence
Representative Public ID CEK012B7R NCBI
Target Description CEK012B7R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F10E7.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27258 /WB_GENE_ID=F10E7.8 /WP=CE04363 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk12b7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063066(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063066 NCBI Caenorhabditis elegans F10E7.8 (F10E7.8) mRNA, complete cds. 11/11 None
F10E7.8 ENSEMBL cdna:known chromosome:CEL140:II:7101196:7105117:-1 gene:F10E7.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7101215-7105114(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F10E7.8
Entrez Gene 174165 Entrez gene
SwissProt Q19300 EMBL-EBI
WormBase CE04363 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604858_AT similar to family with sequence similarity 40, member B cfa
CANINE_2:CFAAFFX.3167.1.S1_S_AT similar to family with sequence similarity 40, member B cfa
CHICKEN:GGAAFFX.22429.1.S1_AT similar to KIAA1170 protein gga
HU35KSUBA:RC_AA011441_AT family with sequence similarity 40, member B hs
U133_X3P:HS2.268044.1.S1_3P_AT family with sequence similarity 40, member B hs
U133_X3P:HS.268044.0.S1_3P_AT family with sequence similarity 40, member B hs
HG-U133_PLUS_2:1555292_AT family with sequence similarity 40, member B hs
HG-U133_PLUS_2:231880_AT family with sequence similarity 40, member B hs
HG-U133B:231880_AT family with sequence similarity 40, member B hs
HG-U95B:43524_AT family with sequence similarity 40, member B hs
Protein Similarities
Method ID Description E-Value
blast AAA82421 Hypothetical protein F10E7.8 [Caenorhabditis elegans] ref|NP_495467.1| F10E7.8 [Caenorhabditis elegans] 0.0
blast CAE67540 Hypothetical protein CBG13065 [Caenorhabditis briggsae] 0.0
blast BAC31154.1 unnamed protein product [Mus musculus] sp|Q8C9H6|FA40B_MOUSE Protein FAM40B 1.0E-114
Sequence
Target Sequence 
>C. ELEGANS:173590_AT
gaagatcttatgtaaatcgattcgatcccgtttccactgaacaaaaatctgtaaataact
aattattagttattaattcttctttaacgataaaaacgagttcatctttattgttccctt
gttccccaatgataactaacttgtaaatcgaaattttgtttgaaattttagatctcccca
tgcccccttatactttaccaaccatcatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGATCTTATGTAAATCGATTCGA 274 337 2789 Antisense
GATCCCGTTTCCACTGAACAAAAAT 499 347 2812 Antisense
CCCGTTTCCACTGAACAAAAATCTG 255 259 2815 Antisense
TAGTTATTAATTCTTCTTTAACGAT 614 651 2855 Antisense
ACGAGTTCATCTTTATTGTTCCCTT 527 93 2884 Antisense
CGAGTTCATCTTTATTGTTCCCTTG 188 249 2885 Antisense
TTCCCTTGTTCCCCAATGATAACTA 503 693 2902 Antisense
GATAACTAACTTGTAAATCGAAATT 579 1 2919 Antisense
TTGAAATTTTAGATCTCCCCATGCC 124 705 2948 Antisense
CCCTTATACTTTACCAACCATCATT 228 243 2973 Antisense
CTTATACTTTACCAACCATCATTTT 201 223 2975 Antisense
Sequence Source Affymetrix Proprietary Database