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[173586_s_at]

Description C41016 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=W02B12.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C41016 /WB_GENE_ID=W02B12.10 /WP=CE03770 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk291d10 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 88      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 96      6grp_total > 78      4grp_total > 59      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 371
1 2 3 4 range
A 961.1
(-2.7)
P 		1026.6
(-0.4)
P 		1122.7
(3)
P 		951.7
(-3)
P 		171
B 973.8
(2.7)
P 		982.5
(3)
P 		961
(2.2)
P 		823.8
(-3)
P 		159
C 1010
(-0.3)
P 		1005.7
(-0.4)
P 		1194.9
(3)
P 		859.2
(-3)
P 		336
avr 981.6
(-0.1) 		1004.9
(0.5) 		1092.9
(3) 		878.2
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 592
1 2 3 4 5 6 range
A 588.2
(-3)
P 		623.4
(-2.6)
P 		632.4
(-2.5)
P 		622.5
(-2.6)
P 		746.3
(-1.4)
P 		1170.3
(3)
P 		582
B 749.6
(-1.4)
P 		631.7
(-2.6)
P 		755.8
(-1.3)
P 		588.2
(-3)
P 		902.6
(0.2)
P 		1179.9
(3)
P 		592
C 616.5
(-2.9)
P 		680.6
(-2.2)
P 		603.3
(-3)
P 		705.1
(-1.9)
P 		857.5
(-0.3)
P 		1158.7
(3)
P 		555
avr 651.4
(-2.9) 		645.2
(-2.9) 		663.8
(-2.7) 		638.6
(-3) 		835.5
(-0.8) 		1169.6
(3) 		531
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 277
1 2 3 4 range
A 349.9
(3)
P 		222.9
(-0.6)
P 		293.5
(1.4)
P 		139.3
(-3)
P 		211
B 310.5
(3)
P 		242.7
(-0)
P 		222.7
(-0.9)
P 		175.5
(-3)
P 		135
C 334.3
(1.2)
P 		289.1
(0.2)
P 		416.5
(3)
P 		144.9
(-3)
P 		272
avr 331.6
(3) 		251.6
(0.3) 		310.9
(2.3) 		153.2
(-3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 108.4
(-3)
P 		221.7
(0.9)
P 		137.7
(-2)
P 		157.9
(-1.3)
P 		225.2
(1)
P 		284.9
(3)
P 		177
B 260.8
(-0.8)
P 		210.3
(-1.8)
P 		183.5
(-2.4)
P 		155.8
(-3)
P 		366.7
(1.5)
P 		440
(3)
P 		284
C 228.5
(-0.9)
P 		207.7
(-1.4)
P 		153.6
(-2.7)
P 		141.8
(-3)
P 		277.5
(0.3)
P 		387.9
(3)
P 		246
avr 199.2
(-1.7) 		213.2
(-1.3) 		158.3
(-2.8) 		151.8
(-3) 		289.8
(0.8) 		370.9
(3) 		219
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173586_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23344
Sequence Type Exemplar sequence
Representative Public ID C41016 NCBI
Target Description C41016 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=W02B12.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C41016 /WB_GENE_ID=W02B12.10 /WP=CE03770 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk291d10 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064047 NCBI Caenorhabditis elegans W02B12.10 (W02B12.10) mRNA, complete cds. 11/11 None
SNAP00000002915 ENSEMBL cdna:SNAP chromosome:CEL140:II:11472164:11473467:-1 11/11 None
GENEFINDER00000002931 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11472164:11473467:-1 11/11 None
W02B12.10 ENSEMBL cdna:known chromosome:CEL140:II:11472104:11473467:-1 gene:W02B12.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018106 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11472111-11473465(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02B12.10
Entrez Gene 174753 Entrez gene
SwissProt Q23126 EMBL-EBI
WormBase CE03770 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246965_AT expressed protein at
CANINE_2:CFAAFFX.1386.1.S1_AT similar to methyltransferase-like protein 1 isoform a cfa
DROSOPHILA_2:1632089_AT dm
DROSGENOME1:153114_AT dm
HG-U133_PLUS_2:204027_S_AT methyltransferase like 1 hs
HG-U133A:204027_S_AT methyltransferase like 1 hs
HG-FOCUS:204027_S_AT methyltransferase like 1 hs
HG-U133A_2:204027_S_AT methyltransferase like 1 hs
HUGENEFL:X96698_AT methyltransferase like 1 hs
U133_X3P:G13186321_3P_A_AT methyltransferase like 1 hs
HG-U95AV2:36001_AT methyltransferase like 1 hs
MOUSE430_2:1419122_AT methyltransferase-like 1 mm
MOE430A:1419122_AT methyltransferase-like 1 mm
MOUSE430A_2:1419122_AT methyltransferase-like 1 mm
MG-U74AV2:99499_AT methyltransferase-like 1 mm
MG-U74CV2:167076_I_AT methyltransferase-like 1 mm
MOE430B:1439155_AT methyltransferase-like 1 mm
MOUSE430_2:1439155_AT methyltransferase-like 1 mm
MOE430B:1447683_X_AT methyltransferase-like 1 mm
MOUSE430_2:1447683_X_AT methyltransferase-like 1 mm
MU19KSUBA:TC15890_AT Methyltransferase-like 1, mRNA (cDNA clone MGC:13767 IMAGE:4020487) mm
MU19KSUBA:TC15890_G_AT Methyltransferase-like 1, mRNA (cDNA clone MGC:13767 IMAGE:4020487) mm
MU19KSUBB:TC26419_AT Methyltransferase-like 1, mRNA (cDNA clone MGC:13767 IMAGE:4020487) mm
YEAST_2:1772827_AT Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91400 Hypothetical protein W02B12.10 [Caenorhabditis elegans] ref|NP_496448.1| W02B12.10 [Caenorhabditis elegans] sp|Q23126|TRMB_CAEEL Probable tRNA (guanine-N(7)-)-methyltransferase (tRNA(m7G46)-methyltransferase) 1.0E-154
blast CAA91400 Hypothetical protein W02B12.10 [Caenorhabditis elegans] ref|NP_496448.1| W02B12.10 [Caenorhabditis elegans] sp|Q23126|TRMB_CAEEL Probable tRNA (guanine-N(7)-)-methyltransferase (tRNA(m7G46)-methyltransferase) 1.0E-148
blast CAE59497 Hypothetical protein CBG02883 [Caenorhabditis briggsae] 1.0E-135
blast CAE59497 Hypothetical protein CBG02883 [Caenorhabditis briggsae] 1.0E-130
blast CAG85186 unnamed protein product [Debaryomyces hansenii CBS767] ref|XP_457191.1| unnamed protein product [Debaryomyces hansenii] 2.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR003358 EMBL-EBI Putative methyltransferase 6.3E-74
Pfam IPR003358 EMBL-EBI Putative methyltransferase 1.9E-27
Pfam IPR003358 EMBL-EBI Putative methyltransferase 7.3E-110
Sequence
Target Sequence 
>C. ELEGANS:173586_S_AT
gagattgtcacctaaatatcctgataatctgatgatcggtatggaaattcgtgtgaaagt
gtcggattacgtaaacgaaaaaatccaagcacttcgaaagcatcacgctgaagctggtca
ctacagaaatgtagccgttctccgatcaaatgccatgaaatacatgccaaattatttcca
caaaggacaactttccaagatgttcttccttttcccagatccacatttcaagaataagaa
gcacaaatggcggatcatcactccaacactgctctccgaatacgcttatgtgcttcgtga
aggaggtattatctacacaatcactgatgtcaaggatctacacgaatggatggtcaagca
tctcagcgagcatccacttttcgaacggctgactgaagaagaaatgaaaaaagatccgat
tgtggaaatgctctttgaaagtactgaagaaggacaaaaagtgactcgaaatgacggagg
aaagtggccagcaatcttcaggcgactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATTGTCACCTAAATATCCTGAT 608 397 259 Antisense
GCATCACGCTGAAGCTGGTCACTAC 198 309 358 Antisense
AAATGTAGCCGTTCTCCGATCAAAT 514 119 385 Antisense
CTTTTCCCAGATCCACATTTCAAGA 664 265 467 Antisense
GCACAAATGGCGGATCATCACTCCA 491 317 499 Antisense
CACTGCTCTCCGAATACGCTTATGT 87 199 525 Antisense
ATACGCTTATGTGCTTCGTGAAGGA 607 21 538 Antisense
TGGTCAAGCATCTCAGCGAGCATCC 82 551 609 Antisense
CATCCACTTTTCGAACGGCTGACTG 132 211 629 Antisense
GATCCGATTGTGGAAATGCTCTTTG 286 417 671 Antisense
GTGGCCAGCAATCTTCAGGCGACTT 385 489 742 Antisense
Sequence Source Affymetrix Proprietary Database