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[173585_at]

Description CEK040G9R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F16D3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34090 /WB_GENE_ID=F16D3.4 /WP=CE09437 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk40g9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 47      4grp_highest_8_8 > 52      4grp_high_8_8 > 17      6grp_highest_nonorm > 76      6grp_total > 48      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 700
1 2 3 4 range
A 1234.9
(3)
P 		859.9
(-1)
P 		670.8
(-3)
P 		1028.4
(0.8)
P 		564
B 1018.6
(3)
P 		725.2
(-2.6)
P 		704.3
(-3)
P 		833.1
(-0.5)
P 		314
C 1371.1
(3)
P 		813.9
(-3)
P 		854.6
(-2.6)
P 		1061.8
(-0.3)
P 		557
avr 1208.2
(3) 		799.7
(-2.3) 		743.2
(-3) 		974.4
(-0) 		465

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1053
1 2 3 4 5 6 range
A 1422.4
(3)
P 		998.8
(-3)
P 		1257.2
(0.7)
P 		1030.3
(-2.6)
P 		1210.8
(0)
P 		1272.2
(0.9)
P 		424
B 1354.2
(1.6)
P 		1239.2
(0.9)
P 		552.3
(-3)
P 		1403.6
(1.9)
P 		912.4
(-0.9)
P 		1605.1
(3)
P 		1053
C 1018.8
(-0.4)
P 		1221.6
(3)
P 		1034.7
(-0.2)
P 		867.6
(-3)
P 		899.3
(-2.5)
P 		935.4
(-1.9)
P 		354
avr 1265.1
(2.9) 		1153.2
(0.8) 		948.1
(-3) 		1100.5
(-0.2) 		1007.5
(-1.9) 		1270.9
(3) 		323
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 303
1 2 3 4 range
A 449.6
(3)
P 		186.7
(-2.3)
P 		175.4
(-2.5)
P 		150.5
(-3)
P 		299
B 324.8
(3)
P 		179.1
(-2.4)
P 		163.2
(-3)
P 		177.5
(-2.5)
P 		162
C 453.9
(3)
P 		234
(-1.8)
P 		297.9
(-0.4)
P 		179
(-3)
P 		275
avr 409.4
(3) 		199.9
(-2.2) 		212.2
(-1.9) 		169
(-3) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 464
1 2 3 4 5 6 range
A 262.2
(-2.9)
P 		355.2
(2.4)
P 		273.8
(-2.3)
P 		261.3
(-3)
P 		365.4
(3)
P 		309.7
(-0.2)
P 		104
B 471.2
(1.4)
P 		412.5
(0.6)
P 		134.1
(-3)
P 		371.8
(0.1)
P 		370.7
(0.1)
P 		598.5
(3)
P 		464
C 377.6
(3)
P 		372.7
(2.9)
P 		263.3
(-0.4)
P 		174.5
(-3)
P 		291
(0.4)
P 		313.1
(1.1)
P 		203
avr 370.3
(1.8) 		380.1
(2.1) 		223.7
(-3) 		269.2
(-1.5) 		342.4
(0.9) 		407.1
(3) 		183
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173585_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23010
Sequence Type Exemplar sequence
Representative Public ID CEK040G9R NCBI
Target Description CEK040G9R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F16D3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34090 /WB_GENE_ID=F16D3.4 /WP=CE09437 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk40g9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059869(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059869 NCBI Caenorhabditis elegans F16D3.4 (F16D3.4) mRNA, complete cds. 11/11 None
F16D3.4 ENSEMBL cdna:known chromosome:CEL140:I:8372087:8378042:-1 gene:F16D3.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036927 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8372028-8377974(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16D3.4
Entrez Gene 172622 Entrez gene
SwissProt Q19493 EMBL-EBI
WormBase CE09437 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01496 Hypothetical protein F16D3.4 [Caenorhabditis elegans] ref|NP_492270.1| F16D3.4 [Caenorhabditis elegans] 0.0
blast CAE60462 Hypothetical protein CBG04073 [Caenorhabditis briggsae] 0.0
blast NP_776619 tubulin-specific chaperone d [Bos taurus] gb|AAB17537.1| cofactor D [Bos taurus] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR007766 EMBL-EBI Beta-tubulin cofactor D 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173585_AT
aattgtgatcctataacgcttgttgacatatttttcaattttatcgcttcgtctagttta
tctttttactttttaattttattcaaatttttgatattttaccactcagaatttgatact
tcacagtgattcgcgtttcgtcttcgccaatttacaactgacaggacagacacaaaaatc
attcattgcgtgttatttatgcggcattttggctcttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTGTGATCCTATAACGCTTGTTG 478 179 3711 Antisense
ATAACGCTTGTTGACATATTTTTCA 563 25 3723 Antisense
TTTCAATTTTATCGCTTCGTCTAGT 194 671 3743 Antisense
ATCGCTTCGTCTAGTTTATCTTTTT 351 37 3753 Antisense
AATTTTTGATATTTTACCACTCAGA 144 175 3796 Antisense
ACCACTCAGAATTTGATACTTCACA 599 85 3811 Antisense
TTCACAGTGATTCGCGTTTCGTCTT 618 685 3830 Antisense
TTTCGTCTTCGCCAATTTACAACTG 137 671 3846 Antisense
TTCGCCAATTTACAACTGACAGGAC 169 625 3853 Antisense
TCATTGCGTGTTATTTATGCGGCAT 133 619 3893 Antisense
TTTATGCGGCATTTTGGCTCTTATT 638 673 3906 Antisense
Sequence Source Affymetrix Proprietary Database