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[173581_s_at]

Description CEC5142 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y41C4A.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C09514 /WB_GENE_ID=Y41C4A.10 /WP=CE20250 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk172c6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 76      6grp_total > 53      4grp_total > 58      6grp_highest_sub > 03      6grp_cgc > 90      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 809
1 2 3 4 range
A 827.3
(-2.1)
P 		1328.7
(3)
P 		1303.1
(2.7)
P 		735.9
(-3)
P 		593
B 989.6
(-0.4)
P 		1239.3
(1.8)
P 		1384.9
(3)
P 		685.7
(-3)
P 		699
C 872.6
(-0.7)
P 		1359.6
(3)
P 		1295.9
(2.5)
P 		576.1
(-3)
P 		784
avr 896.5
(-0.9) 		1309.2
(2.8) 		1328
(3) 		665.9
(-3) 		662

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 597
1 2 3 4 5 6 range
A 913.8
(-3)
P 		1036.9
(1.4)
P 		1041.1
(1.5)
P 		989
(-0.3)
P 		1019
(0.7)
P 		1082.9
(3)
P 		169
B 899.7
(-1.6)
P 		756.5
(-3)
P 		1353.3
(3)
P 		973.2
(-0.8)
P 		998.9
(-0.6)
P 		1015.1
(-0.4)
P 		597
C 1327.4
(3)
P 		809.1
(-3)
P 		1114.3
(0.5)
P 		1084
(0.2)
P 		1080.9
(0.1)
P 		858.5
(-2.4)
P 		518
avr 1047
(0.6) 		867.5
(-3) 		1169.6
(3) 		1015.4
(-0.1) 		1032.9
(0.3) 		985.5
(-0.7) 		302
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 301.2
(2)
P 		288.4
(1.7)
P 		340.7
(3)
P 		107.7
(-3)
P 		233
B 315.6
(2.8)
P 		306.1
(2.5)
P 		320.9
(3)
P 		146.1
(-3)
P 		175
C 288.9
(0.2)
P 		390.9
(2)
P 		451.7
(3)
P 		97.1
(-3)
P 		355
avr 301.9
(1.4) 		328.5
(2) 		371.1
(3) 		117
(-3) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 168.5
(-3)
P 		368.8
(3)
P 		226.7
(-1.3)
P 		250.8
(-0.5)
P 		307.5
(1.2)
P 		263.6
(-0.2)
P 		200
B 313.1
(-0.6)
P 		251.8
(-3)
P 		328.6
(-0)
P 		257.8
(-2.8)
P 		405.9
(3)
P 		378.5
(1.9)
P 		154
C 492
(3)
P 		246.9
(-2.4)
P 		283.6
(-1.6)
P 		218
(-3)
P 		349.7
(-0.1)
P 		287.4
(-1.5)
P 		274
avr 324.5
(1.4) 		289.2
(-0.5) 		279.6
(-1) 		242.2
(-3) 		354.4
(3) 		309.8
(0.6) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173581_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24109
Sequence Type Exemplar sequence
Representative Public ID CEC5142 NCBI
Target Description CEC5142 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y41C4A.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C09514 /WB_GENE_ID=Y41C4A.10 /WP=CE20250 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk172c6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067116(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067116 NCBI Caenorhabditis elegans ELongin B family member (elb-1) (elb-1) mRNA, complete cds. 11/11 None
SNAP00000034891 ENSEMBL cdna:SNAP chromosome:CEL140:III:11718864:11719316:-1 11/11 None
GENEFINDER00000034904 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11718864:11719316:-1 11/11 None
Y41C4A.10 ENSEMBL cdna:known chromosome:CEL140:III:11718711:11719326:-1 gene:Y41C4A.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11718739-11719313(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol elb-1
Ensembl Y41C4A.10
Entrez Gene 176605 Entrez gene
SwissProt Q9XWU4 EMBL-EBI
WormBase CE20250 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21548 Hypothetical protein Y41C4A.10 [Caenorhabditis elegans] ref|NP_499517.1| ELongin B family member (elb-1) [Caenorhabditis elegans] gb|AAG49392.1| RNA polymerase II transcription factor SIII p18 subunit [Caenorhabditis elegans] 4.0E-62
blast CAE67673 Hypothetical protein CBG13236 [Caenorhabditis briggsae] 2.0E-44
blast XP_797447 PREDICTED: similar to transcription elongation factor B (SIII), polypeptide 2 [Strongylocentrotus purpuratus] 3.0E-13
Sequence
Target Sequence 
>C. ELEGANS:173581_S_AT
tcccaacaggagctattcttcgaggtgcgccgcaacagatcgcacatttacctcgacgcc
gaggaatctcactcggttatggagctcaagctaatgctcgccggaatcacacgggaccca
gtcaatcatatggagctgtggaaactcgacgaggacggcaacaaaagccagctacttcac
gacaccgccacgctttctgacgccggcttctcgtcgactaatgccaaggcccaatgccca
gcggctctcggactccgactcaccaacgccgaggagcatctcacgatcagcgacgtctcc
actgctccaccaattccagacacaatgcgccaggagcaggctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCAACAGGAGCTATTCTTCGAGG 65 597 17 Antisense
TTCGAGGTGCGCCGCAACAGATCGC 602 695 35 Antisense
AACAGATCGCACATTTACCTCGACG 190 137 50 Antisense
CCTCGACGCCGAGGAATCTCACTCG 553 267 67 Antisense
GGAGCTCAAGCTAATGCTCGCCGGA 362 521 97 Antisense
AATGCTCGCCGGAATCACACGGGAC 313 165 109 Antisense
CACACGGGACCCAGTCAATCATATG 508 195 124 Antisense
ACAAAAGCCAGCTACTTCACGACAC 666 109 177 Antisense
CGACTAATGCCAAGGCCCAATGCCC 112 253 231 Antisense
CCGAGGAGCATCTCACGATCAGCGA 563 263 285 Antisense
GACACAATGCGCCAGGAGCAGGCTC 606 365 335 Antisense
Sequence Source Affymetrix Proprietary Database