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[173580_s_at]

Description CEK101G8F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=W06D12.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D70321 /WB_GENE_ID=W06D12.2 /WP=CE16550 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk101g8 : 5prime end, single read.
Cluster 6grp_total > 41      4grp_total > 59      6grp_cgc > 84      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 126.7
(-0.9)
A 		153.7
(1)
A 		183.5
(3)
A 		95.8
(-3)
A 		88
B 159.4
(1.5)
A 		167.2
(3)
A 		136.8
(-3)
A 		139.4
(-2.5)
A 		30
C 133.4
(-0.5)
A 		164.8
(1.7)
A 		184.3
(3)
M 		96.2
(-3)
A 		88
avr 139.8
(0) 		161.9
(2.3) 		168.2
(3) 		110.5
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 119.2
(-1.1)
A 		192.7
(2.1)
M 		212.2
(3)
M 		193.7
(2.2)
A 		138.6
(-0.3)
A 		77
(-3)
A 		135
B 165.1
(1.2)
A 		193.1
(2.1)
A 		199.4
(2.3)
A 		223.1
(3)
A 		154.9
(0.9)
A 		29.2
(-3)
A 		194
C 213.8
(2.3)
M 		176.8
(0.9)
A 		214.8
(2.4)
A 		231.6
(3)
A 		170.9
(0.7)
A 		73.6
(-3)
A 		158
avr 166
(1.1) 		187.5
(1.9) 		208.8
(2.7) 		216.1
(3) 		154.8
(0.6) 		59.9
(-3) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 46.1
(2.7)
A 		33.4
(0.4)
A 		48
(3)
A 		14
(-3)
A 		34
B 50.8
(3)
A 		41.3
(0.3)
A 		31.7
(-2.4)
A 		29.7
(-3)
A 		21
C 44.2
(0.5)
A 		47.4
(0.9)
A 		64.2
(3)
M 		16.2
(-3)
A 		48
avr 47
(2.8) 		40.7
(1.4) 		48
(3) 		20
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 22
(-2.6)
A 		68.5
(3)
M 		46.2
(0.3)
M 		49.1
(0.7)
A 		41.8
(-0.2)
A 		18.7
(-3)
A 		50
B 57.5
(2.2)
A 		64.3
(3)
A 		48.4
(1.2)
A 		59.1
(2.4)
A 		62.9
(2.8)
A 		10.9
(-3)
A 		53
C 79.2
(3)
M 		54
(0.2)
A 		54.7
(0.3)
A 		46.6
(-0.6)
A 		55.3
(0.4)
A 		24.6
(-3)
A 		55
avr 52.9
(1.7) 		62.3
(3) 		49.8
(1.3) 		51.6
(1.5) 		53.3
(1.8) 		18.1
(-3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173580_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25603
Sequence Type Exemplar sequence
Representative Public ID CEK101G8F NCBI
Target Description CEK101G8F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=W06D12.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D70321 /WB_GENE_ID=W06D12.2 /WP=CE16550 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk101g8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075082(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075082 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-42) (twk-42) mRNA, complete cds. 11/11 None
SNAP00000010708 ENSEMBL cdna:SNAP chromosome:CEL140:V:17725301:17731321:-1 11/11 None
GENEFINDER00000010714 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17729399:17732947:-1 11/11 None
W06D12.2 ENSEMBL cdna:known chromosome:CEL140:V:17729399:17735102:-1 gene:W06D12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17729399-17732948(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-42
Ensembl W06D12.2
Entrez Gene 180163 Entrez gene
SwissProt O45891 EMBL-EBI
WormBase CE16550 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04923 Hypothetical protein W06D12.2 [Caenorhabditis elegans] ref|NP_507483.1| TWiK family of potassium channels family member (twk-42) [Caenorhabditis elegans] 0.0
blast CAE56818 Hypothetical protein CBG24632 [Caenorhabditis briggsae] 0.0
blast CAB04923 Hypothetical protein W06D12.2 [Caenorhabditis elegans] ref|NP_507483.1| TWiK family of potassium channels family member (twk-42) [Caenorhabditis elegans] 1.0E-125
blast CAE56818 Hypothetical protein CBG24632 [Caenorhabditis briggsae] 1.0E-116
blast CAE75434 Hypothetical protein CBG23427 [Caenorhabditis briggsae] 6.0E-29
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507483.1 5 15-37,112-131,135-157,225-247,277-299
W06D12.2 5 15-37,112-131,135-157,225-247,277-299
SNAP00000010708 3 36-58,65-84,89-111
GENEFINDER00000010714 5 15-37,112-131,135-157,225-247,277-299
Sequence
Target Sequence 
>C. ELEGANS:173580_S_AT
ctggatgatccaatgcgttgcctactttgcatactttgagaactggacacttttcgagtc
agtctactttttcttcatctccatgacaaccataggattcggagattttaccccgagcca
tacagtggcagttggtggaatcgtgttcatccttggaggtctctctgtggtttccatgtg
cattaatgtaatacaaatgcagttggagttcattttcaaccagattgtacagagaattga
gaacgattttaagaacactttgagtgtggcagccgaggagagccgcaaaaagtcaatcgg
agtctccgaattcggctctatcgatccctccaagaaaaagacaatgagaaccgacggtaa
tgtggcagagaagtacagtgagaatatggagatgggaaataagcttttgatgcgatttat
gagcaatcatcagaagaagatgttgaacgagaagtttgacgagcgggcgaagatgaggaa
tagtggaacgcagacaacgtcgcagattaaggttgccagtgtccaaaccgcagaacgcta
tga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGATGATCCAATGCGTTGCCTAC 297 241 718 Antisense
GCGTTGCCTACTTTGCATACTTTGA 22 291 732 Antisense
CGAGTCAGTCTACTTTTTCTTCATC 45 251 772 Antisense
TTTCTTCATCTCCATGACAACCATA 607 671 787 Antisense
ATTTTACCCCGAGCCATACAGTGGC 185 3 822 Antisense
GGTGGAATCGTGTTCATCCTTGGAG 180 499 851 Antisense
ATCCTTGGAGGTCTCTCTGTGGTTT 349 39 866 Antisense
GAGTGTGGCAGCCGAGGAGAGCCGC 559 401 979 Antisense
ATCGGAGTCTCCGAATTCGGCTCTA 333 35 1013 Antisense
GTGGAACGCAGACAACGTCGCAGAT 279 491 1200 Antisense
GTGTCCAAACCGCAGAACGCTATGA 648 485 1236 Antisense
Sequence Source Affymetrix Proprietary Database