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[173556_s_at]

Description CEK011EZF /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C06A8.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34708 /WB_GENE_ID=C06A8.1 /WP=CE02451 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11e12 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 45      6grp_total > 91      4grp_total > 48      6grp_highest_sub > 04_05      6grp_high1_sub > 04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3415
1 2 3 4 range
A 759.2
(-3)
P 		3159.3
(1.5)
P 		3994.5
(3)
P 		2150.7
(-0.4)
P 		3235
B 724.6
(-3)
P 		3021.5
(1.3)
P 		3908.4
(3)
P 		2238
(-0.1)
P 		3184
C 673.9
(-3)
P 		2955.2
(1)
P 		4089.1
(3)
P 		1788.1
(-1)
P 		3415
avr 719.2
(-3) 		3045.3
(1.3) 		3997.3
(3) 		2058.9
(-0.5) 		3278

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1569
1 2 3 4 5 6 range
A 1534.1
(-3)
P 		1997.5
(-1)
P 		1744.8
(-2.1)
P 		2931.4
(3)
P 		2730.2
(2.1)
P 		1863.8
(-1.6)
P 		1397
B 1565.6
(-2.5)
P 		1839.9
(-1.2)
P 		1473.7
(-3)
P 		2679.5
(3)
P 		2553.6
(2.4)
P 		1630.4
(-2.2)
P 		1206
C 1620.1
(-3)
P 		1679.6
(-2.7)
P 		2011.6
(-1.3)
P 		2721.2
(1.6)
P 		3043.1
(3)
P 		1799.1
(-2.2)
P 		1423
avr 1573.3
(-3) 		1839
(-1.7) 		1743.4
(-2.2) 		2777.4
(3) 		2775.6
(3) 		1764.4
(-2) 		1204
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1202
1 2 3 4 range
A 276.4
(-3)
P 		685.8
(0.2)
P 		1044.2
(3)
P 		314.8
(-2.7)
P 		768
B 231.1
(-3)
P 		746.2
(1.6)
P 		905.7
(3)
P 		476.9
(-0.8)
P 		675
C 223.1
(-3)
P 		849.7
(0.1)
P 		1425.2
(3)
P 		301.5
(-2.6)
P 		1202
avr 243.5
(-3) 		760.6
(0.5) 		1125
(3) 		364.4
(-2.2) 		882

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 755
1 2 3 4 5 6 range
A 282.8
(-3)
P 		710.4
(1.7)
P 		380
(-1.9)
P 		743.5
(2.1)
P 		823.8
(3)
P 		453.8
(-1.1)
P 		541
B 544.7
(-1.4)
P 		612.4
(-0.8)
P 		357.9
(-3)
P 		709.7
(0.1)
P 		1037.5
(3)
P 		608
(-0.8)
P 		680
C 600.5
(-1.9)
P 		512.5
(-3)
P 		512
(-3)
P 		547.2
(-2.6)
P 		984.7
(3)
P 		602.3
(-1.9)
P 		473
avr 476
(-2.3) 		611.8
(-0.8) 		416.6
(-3) 		666.8
(-0.2) 		948.7
(3) 		554.7
(-1.4) 		532
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173556_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23563
Sequence Type Exemplar sequence
Representative Public ID CEK011EZF NCBI
Target Description CEK011EZF /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C06A8.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34708 /WB_GENE_ID=C06A8.1 /WP=CE02451 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk11e12 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171024(11), NM_171025(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171024 NCBI Caenorhabditis elegans C06A8.1a (C06A8.1) mRNA, complete cds. 11/11 None
NM_171025 NCBI Caenorhabditis elegans C06A8.1b (C06A8.1) mRNA, complete cds. 11/11 None
SNAP00000042208 ENSEMBL cdna:SNAP chromosome:CEL140:II:7772496:7776291:-1 11/11 None
GENEFINDER00000042224 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7774062:7777670:-1 11/11 None
C06A8.1a.2 ENSEMBL cdna:known chromosome:CEL140:II:7773801:7777744:-1 gene:C06A8.1 11/11 None
C06A8.1a.1 ENSEMBL cdna:known chromosome:CEL140:II:7773801:7777753:-1 gene:C06A8.1 11/11 None
C06A8.1b ENSEMBL cdna:known chromosome:CEL140:II:7774062:7777733:-1 gene:C06A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7774059-7777668(-) 100.0 100.0
Public Domain and Genome References
Ensembl C06A8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81048 Hypothetical protein C06A8.1a [Caenorhabditis elegans] ref|NP_741027.1| C06A8.1a [Caenorhabditis elegans] sp|Q17693|MTHR_CAEEL Probable methylenetetrahydrofolate reductase 0.0
blast AAM81124 Hypothetical protein C06A8.1b [Caenorhabditis elegans] ref|NP_741028.1| C06A8.1b [Caenorhabditis elegans] 0.0
blast CAE67459 Hypothetical protein CBG12960 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003171 EMBL-EBI Methylenetetrahydrofolate reductase 1.0E-126
Pfam IPR003171 EMBL-EBI Methylenetetrahydrofolate reductase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173556_S_AT
tcaagctccaaacgccgatggcgtcaaggttactgtacttccatggacagaagctgaaac
tggtgttcagccagaaacttcattgatcagtgaacaacttgtttggtgtaatgagaatgg
aattctgaccgtcaacagtcagccatcagttaatggagctccatccactgatccacttgt
tgggtggggaaaaccaggaggttactgctaccaaaaggcatacctcgaatgcttcatgac
cgcggagttgtccgacaagctcattcaaatcatcgagagagaattcccagtcagagttaa
ctaccatgccatcaataaggagagtaccttcgacaagaccaacagcgaagaaaccactcc
gattgcagttacttggggagtattcccaggatctgagattgcccagccaacagtcgtcga
tccactctctttccgcgcatggagagatgaagcttaccaaatgtggatggcccaatgggg
agatttttatccaaaagaatctaaatcatatggagtcatcaaggctgttcatgatgagtt
ccgtttggttactctcgtcgacaacgacttccagaagccatcagttcttttcgacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAGCTCCAAACGCCGATGGCGTC 528 631 1231 Antisense
GGCGTCAAGGTTACTGTACTTCCAT 215 539 1250 Antisense
TGACCGTCAACAGTCAGCCATCAGT 703 563 1356 Antisense
CCATCCACTGATCCACTTGTTGGGT 358 271 1391 Antisense
CATACCTCGAATGCTTCATGACCGC 336 213 1449 Antisense
ATGACCGCGGAGTTGTCCGACAAGC 344 45 1466 Antisense
CAGAGTTAACTACCATGCCATCAAT 87 207 1522 Antisense
GAAACCACTCCGATTGCAGTTACTT 382 359 1580 Antisense
TGAGTTCCGTTTGGTTACTCTCGTC 265 565 1765 Antisense
TACTCTCGTCGACAACGACTTCCAG 470 643 1780 Antisense
AGAAGCCATCAGTTCTTTTCGACGT 674 73 1803 Antisense
Sequence Source Affymetrix Proprietary Database