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[173550_at]

Description C58301_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F45D3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C58301 /WB_GENE_ID=F45D3.4 /WP=CE10474 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk364e9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 97      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 58      6grp_total > 96      4grp_total > 23      6grp_highest_sub > Cu      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3535
1 2 3 4 range
A 4937.5
(3)
P 		1875
(-3)
P 		2147.7
(-2.5)
P 		3514.7
(0.2)
P 		3063
B 4421.4
(3)
P 		2023
(-1.8)
P 		1402.5
(-3)
P 		4094.6
(2.4)
P 		3019
C 3893.1
(3)
P 		2313.7
(-2)
P 		2014.1
(-3)
P 		3792.1
(2.7)
P 		1879
avr 4417.3
(3) 		2070.6
(-2.5) 		1854.8
(-3) 		3800.5
(1.6) 		2563

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3577
1 2 3 4 5 6 range
A 2257.7
(-1.8)
P 		2766.3
(-0.8)
P 		1686.2
(-3)
P 		3116.7
(-0.1)
P 		3333.2
(0.4)
P 		4629.2
(3)
P 		2943
B 2260.9
(-0.6)
P 		2499.2
(-0.1)
P 		1052.4
(-3)
P 		2576.4
(0)
P 		3131
(1.1)
P 		4062.9
(3)
P 		3011
C 2780.6
(-0.5)
P 		2111.1
(-3)
P 		2272.5
(-2.4)
P 		3371.7
(1.7)
P 		3604.9
(2.6)
P 		3722.4
(3)
P 		1611
avr 2433.1
(-1.1) 		2458.9
(-1.1) 		1670.4
(-3) 		3021.6
(0.3) 		3356.4
(1.1) 		4138.2
(3) 		2468
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1473
1 2 3 4 range
A 1797.7
(3)
P 		407
(-3)
P 		561.5
(-2.3)
P 		514.5
(-2.5)
P 		1391
B 1409.9
(3)
P 		499.6
(-2)
P 		325
(-3)
P 		872.6
(0)
P 		1085
C 1288.7
(3)
P 		665.2
(-2.8)
P 		702
(-2.4)
P 		639.4
(-3)
P 		649
avr 1498.8
(3) 		523.9
(-3) 		529.5
(-3) 		675.5
(-2.1) 		975

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1259
1 2 3 4 5 6 range
A 416.2
(-2.6)
P 		983.8
(1.9)
P 		367.2
(-3)
P 		790.5
(0.3)
P 		1005.8
(2)
P 		1127.1
(3)
P 		760
B 786.7
(-0.5)
P 		831.9
(-0.3)
P 		255.6
(-3)
P 		682.4
(-1)
P 		1272.1
(1.8)
P 		1515
(3)
P 		1259
C 1030.6
(1.1)
P 		644.2
(-2.4)
P 		578.4
(-3)
P 		678
(-2.1)
P 		1166.5
(2.3)
P 		1246.1
(3)
P 		668
avr 744.5
(-0.7) 		820
(-0.2) 		400.4
(-3) 		717
(-0.9) 		1148.1
(2) 		1296.1
(3) 		896
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173550_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25288
Sequence Type Exemplar sequence
Representative Public ID C58301 NCBI
Target Description C58301_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F45D3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C58301 /WB_GENE_ID=F45D3.4 /WP=CE10474 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk364e9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028759(11), NM_001028760(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028759 NCBI Caenorhabditis elegans F45D3.4a (F45D3.4) mRNA, complete cds. 11/11 None
NM_001028760 NCBI Caenorhabditis elegans F45D3.4b (F45D3.4) mRNA, complete cds. 11/11 None
F45D3.4 ENSEMBL cdna:known chromosome:CEL140:V:12554737:12555817:-1 gene:F45D3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12554877-12555755(-) 100.0 100.0
Public Domain and Genome References
Ensembl F45D3.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01501 Hypothetical protein F45D3.4a [Caenorhabditis elegans] ref|NP_001023930.1| F45D3.4a [Caenorhabditis elegans] 1.0E-109
blast CAE75511 Hypothetical protein CBG23525 [Caenorhabditis briggsae] 2.0E-97
blast CAI91164 Hypothetical protein F45D3.4b [Caenorhabditis elegans] ref|NP_001023931.1| F45D3.4b [Caenorhabditis elegans] 3.0E-88
blast CAB01501 Hypothetical protein F45D3.4a [Caenorhabditis elegans] ref|NP_001023930.1| F45D3.4a [Caenorhabditis elegans] 2.0E-82
blast CAI91164 Hypothetical protein F45D3.4b [Caenorhabditis elegans] ref|NP_001023931.1| F45D3.4b [Caenorhabditis elegans] 1.0E-81
blast CAE75511 Hypothetical protein CBG23525 [Caenorhabditis briggsae] 3.0E-73
Sequence
Target Sequence 
>C. ELEGANS:173550_AT
aaatcagttgattgtttgatgctcttcacttctatcatttttcatctgtacattcattct
tcaacttgcttctgcttctgtaattgtacctaccattttctta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCAGTTGATTGTTTGATGCTCT 640 117 699 Antisense
TTGATGCTCTTCACTTCTATCATTT 605 705 714 Antisense
TCACTTCTATCATTTTTCATCTGTA 457 627 724 Antisense
TCTATCATTTTTCATCTGTACATTC 350 615 729 Antisense
TTTCATCTGTACATTCATTCTTCAA 82 673 738 Antisense
CTTCAACTTGCTTCTGCTTCTGTAA 82 223 757 Antisense
TCAACTTGCTTCTGCTTCTGTAATT 237 631 759 Antisense
CTTGCTTCTGCTTCTGTAATTGTAC 186 219 763 Antisense
TGCTTCTGCTTCTGTAATTGTACCT 584 579 765 Antisense
CTGCTTCTGTAATTGTACCTACCAT 673 235 770 Antisense
TGTAATTGTACCTACCATTTTCTTA 515 559 777 Antisense
Sequence Source Affymetrix Proprietary Database