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[173543_at]

Description CEK005C2F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C13F10.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27861 /WB_GENE_ID=C13F10.4 /WP=CE08145 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk5c2 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 49      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 82      6grp_total > 38      4grp_total > 23      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 208
1 2 3 4 range
A 299.2
(-0.7)
P 		319.8
(3)
P 		286.4
(-3)
P 		307.5
(0.8)
P 		33
B 461.2
(2.7)
P 		263.6
(-3)
P 		275.5
(-2.7)
P 		471.2
(3)
P 		208
C 386.6
(3)
P 		312.3
(-3)
P 		343.8
(-0.5)
P 		330.6
(-1.5)
P 		74
avr 382.3
(3) 		298.6
(-3) 		301.9
(-2.8) 		369.8
(2.1) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 553.6
(3)
P 		434
(0.4)
P 		316.4
(-2.1)
P 		277
(-3)
P 		378.9
(-0.8)
P 		364.7
(-1.1)
P 		277
B 510.1
(3)
P 		500.5
(2.8)
P 		211.9
(-3)
P 		403.8
(0.9)
P 		306
(-1.1)
P 		500.9
(2.8)
P 		298
C 428.6
(1.6)
P 		433.3
(1.7)
P 		246.8
(-3)
P 		389.4
(0.6)
P 		389.3
(0.6)
P 		484.5
(3)
P 		238
avr 497.4
(3) 		455.9
(2) 		258.4
(-3) 		356.7
(-0.5) 		358.1
(-0.5) 		450
(1.8) 		239
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 108.9
(3)
P 		69.4
(-0.7)
P 		74.9
(-0.2)
P 		45
(-3)
P 		64
B 147.1
(3)
P 		65.1
(-2.9)
P 		63.8
(-3)
P 		100.4
(-0.4)
P 		83
C 128
(3)
P 		89.8
(-0.2)
P 		119.8
(2.3)
P 		55.7
(-3)
P 		72
avr 128
(3) 		74.8
(-2.2) 		86.2
(-1.1) 		67
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 102.1
(-0.7)
P 		154.3
(3)
P 		68.9
(-3)
P 		70.2
(-2.9)
P 		114.3
(0.2)
P 		88.8
(-1.6)
P 		85
B 177.5
(2.6)
P 		166.6
(2.1)
P 		51.4
(-3)
P 		107
(-0.5)
P 		124.3
(0.2)
P 		186.8
(3)
P 		135
C 158.9
(2.8)
P 		132.2
(1.2)
P 		62.8
(-3)
P 		78.3
(-2.1)
P 		126
(0.8)
P 		162.2
(3)
P 		99
avr 146.2
(2.7) 		151
(3) 		61
(-3) 		85.2
(-1.4) 		121.5
(1) 		145.9
(2.7) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173543_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25242
Sequence Type Exemplar sequence
Representative Public ID CEK005C2F NCBI
Target Description CEK005C2F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C13F10.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27861 /WB_GENE_ID=C13F10.4 /WP=CE08145 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk5c2 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072427 NCBI Caenorhabditis elegans C13F10.4 (C13F10.4) mRNA, complete cds. 11/11 None
C13F10.4 ENSEMBL cdna:known chromosome:CEL140:V:7208214:7217480:-1 gene:C13F10.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7208239-7217482(-) 99.37 100.0
Public Domain and Genome References
Gene Title
Ensembl C13F10.4
Entrez Gene 179109 Entrez gene
SwissProt O01485 EMBL-EBI
WormBase CE08145 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9986.1.S1_S_AT similar to CG2747-PB, isoform B cfa
DROSOPHILA_2:1629748_S_AT dm
DROSGENOME1:149470_AT dm
CHICKEN:GGA.7454.1.S1_AT similar to KIAA1414 protein gga
HG-U133_PLUS_2:233642_S_AT KIAA1414 protein hs
HG-U133B:233642_S_AT KIAA1414 protein hs
HU35KSUBA:H59417_S_AT KIAA1414 protein hs
HU35KSUBA:D82385_AT KIAA1414 protein hs
HU35KSUBB:RC_N66289_I_AT KIAA1414 protein hs
HU35KSUBD:RC_H73161_F_AT KIAA1414 protein hs
U133_X3P:HS.92991.1.S1_3P_A_AT KIAA1414 protein hs
HG-U95B:55631_AT KIAA1414 protein hs
HG-U95D:89435_AT KIAA1414 protein hs
U133_X3P:HS2.363439.1.S1_3P_X_AT KIAA1414 protein hs
U133_X3P:HS2.363439.1.S1_3P_AT KIAA1414 protein hs
U133_X3P:1569503_3P_AT KIAA1414 protein hs
HG-U133_PLUS_2:1569503_AT KIAA1414 protein hs
HU35KSUBC:RC_H84644_AT KIAA1414 protein hs
MOUSE430A_2:1420449_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1420449_AT RIKEN cDNA A230048G03 gene mm
MOE430A:1420449_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1439815_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1439815_AT RIKEN cDNA A230048G03 gene mm
MG-U74CV2:138396_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1438085_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1438085_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1459634_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1459634_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1459257_AT Premature mRNA for mKIAA1414 protein mm
MOUSE430_2:1459257_AT Premature mRNA for mKIAA1414 protein mm
RAE230B:1383710_AT similar to KIAA1414 protein rn
RAT230_2:1383710_AT similar to KIAA1414 protein rn
Protein Similarities
Method ID Description E-Value
blast AAC47965 Hypothetical protein C13F10.4 [Caenorhabditis elegans] ref|NP_504828.1| C13F10.4 [Caenorhabditis elegans] 0.0
blast CAE72021 Hypothetical protein CBG19103 [Caenorhabditis briggsae] 0.0
blast XP_863392 PREDICTED: similar to CG2747-PB, isoform B isoform 7 [Canis familiaris] 0.0
Sequence
Target Sequence 
>C. ELEGANS:173543_AT
ggaatacttctgctcaattcctcctactgaactcaacgcgtgcaatatacacacttttca
ttacggttctcttttttttgcagctcatagatattaaaataattttctttctgtatttat
tgtgtaactttttttccacatttttggttcatctctgtgattgttgacttttactagtga
ttttcaagtttttgtttctggaaactcaatctgttctgaacccattctcactcttttttc
cattgattcgtgttctattctaaaatcctatgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATACTTCTGCTCAATTCCTCCT 447 533 6247 Antisense
TCCTACTGAACTCAACGCGTGCAAT 128 545 6268 Antisense
AACGCGTGCAATATACACACTTTTC 384 145 6281 Antisense
ACACACTTTTCATTACGGTTCTCTT 125 111 6295 Antisense
TTACGGTTCTCTTTTTTTTGCAGCT 348 683 6307 Antisense
GTTCTCTTTTTTTTGCAGCTCATAG 427 443 6312 Antisense
TATTGTGTAACTTTTTTTCCACATT 597 665 6364 Antisense
ATCTCTGTGATTGTTGACTTTTACT 25 33 6397 Antisense
TCTTTTTTCCATTGATTCGTGTTCT 677 615 6478 Antisense
TCCATTGATTCGTGTTCTATTCTAA 452 591 6485 Antisense
GTGTTCTATTCTAAAATCCTATGTA 661 485 6496 Antisense
Sequence Source Affymetrix Proprietary Database