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[173539_at]

Description C43485 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=B0511.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43485 /WB_GENE_ID=B0511.10 /WP=CE17349 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk328e6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 26      4grp_highest_8_8 > 13      4grp_high_8_8 > 23      6grp_highest_nonorm > 90      6grp_total > 26      4grp_total > 24      6grp_highest_sub > De      6grp_cgc > 14      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 326
1 2 3 4 range
A 285.8
(-3)
P 		335.7
(-1.8)
P 		415.3
(0)
P 		544
(3)
P 		258
B 218.1
(-3)
P 		298.3
(-1.4)
P 		432
(1.3)
P 		515.6
(3)
P 		298
C 238.3
(-3)
P 		350.8
(-0.4)
P 		400.6
(0.8)
P 		496.9
(3)
P 		259
avr 247.4
(-3) 		328.3
(-1.2) 		416
(0.7) 		518.8
(3) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 225.5
(3)
P 		140.7
(-1.1)
P 		100.2
(-3)
P 		113.8
(-2.3)
P 		107.3
(-2.7)
P 		124.7
(-1.8)
P 		125
B 199.8
(1.7)
P 		214.7
(3)
P 		164.3
(-1.2)
P 		175.3
(-0.3)
P 		143.3
(-3)
P 		155.2
(-2)
P 		71
C 210.6
(3)
P 		200.8
(2.4)
P 		130.9
(-1.6)
P 		128
(-1.8)
P 		107.6
(-3)
P 		157.5
(-0.1)
P 		103
avr 212
(3) 		185.4
(1.3) 		131.8
(-2.2) 		139
(-1.7) 		119.4
(-3) 		145.8
(-1.3) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 104.1
(2.2)
P 		72.9
(-3)
P 		108.6
(3)
P 		79.6
(-1.9)
P 		36
B 69.6
(-3)
P 		73.7
(-2.4)
P 		100.1
(1.5)
P 		109.9
(3)
P 		40
C 78.9
(-3)
P 		100.8
(-0.8)
P 		139.6
(3)
P 		83.8
(-2.5)
P 		61
avr 84.2
(-2.7) 		82.5
(-3) 		116.1
(3) 		91.1
(-1.5) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 41.6
(1.2)
P 		50
(3)
P 		21.8
(-3)
P 		28.9
(-1.5)
P 		32.4
(-0.7)
P 		30.4
(-1.2)
P 		28
B 69.5
(2.6)
P 		71.5
(3)
P 		39.9
(-3)
P 		46.4
(-1.8)
P 		58.2
(0.5)
P 		57.9
(0.4)
P 		32
C 78.1
(3)
P 		61.3
(1.1)
P 		33.3
(-2.1)
P 		25.7
(-3)
P 		34.8
(-2)
P 		52.7
(0.1)
P 		52
avr 63.1
(3) 		60.9
(2.6) 		31.7
(-3) 		33.7
(-2.6) 		41.8
(-1.1) 		47
(-0.1) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22928
Sequence Type Exemplar sequence
Representative Public ID C43485 NCBI
Target Description C43485 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=B0511.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43485 /WB_GENE_ID=B0511.10 /WP=CE17349 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk328e6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060384(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060384 NCBI Caenorhabditis elegans Eukaryotic Initiation Factor family member (eif-3.E) (eif-3.E) mRNA, complete cds. 11/11 None
B0511.10 ENSEMBL cdna:known chromosome:CEL140:I:10648631:10650402:-1 gene:B0511.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10648635-10650328(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol eif-3.E
Ensembl B0511.10
Entrez Gene 172959 Entrez gene
SwissProt O61820 EMBL-EBI
WormBase CE17349 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251628_AT eukaryotic translation initiation factor 3E / eIF3e (TIF3E1) at
CANINE:1603359_AT similar to eukaryotic translation initiation factor 3, subunit 6 (predicted) cfa
CANINE:1590286_AT similar to eukaryotic translation initiation factor 3, subunit 6 (predicted) cfa
CANINE_2:CFA.14462.2.S1_S_AT similar to eukaryotic translation initiation factor 3, subunit 6 (predicted) cfa
CANINE_2:CFAAFFX.1984.1.S1_S_AT similar to eukaryotic translation initiation factor 3, subunit 6 (predicted) cfa
DROSOPHILA_2:1640174_AT Int6 homologue dm
DROSGENOME1:153110_AT Int6 homologue dm
CHICKEN:GGAAFFX.11932.1.S1_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa gga
CHICKEN:GGA.4804.1.S1_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa gga
HG-U95AV2:38681_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133A:208697_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133_PLUS_2:208697_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133A_2:208697_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-FOCUS:208697_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HU35KSUBA:R56183_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HU35KSUBC:RC_R61297_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HUGENEFL:U62962_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
U133_X3P:G12653884_3P_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U95B:43843_S_AT eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133_PLUS_2:235429_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133B:235429_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133_PLUS_2:236989_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U133B:236989_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U95B:59337_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U95C:61054_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HG-U95D:78729_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HU35KSUBB:RC_AA069547_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
HU35KSUBD:RC_N21321_I_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
U133_X3P:HS.161623.0.A1_3P_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
U133_X3P:HS.293171.0.A1_3P_AT Eukaryotic translation initiation factor 3, subunit 6 48kDa hs
MOE430A:1434523_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430A_2:1434523_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430_2:1434523_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430A_2:1439268_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOE430A:1439268_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430_2:1439268_X_AT eukaryotic translation initiation factor 3, subunit 6 mm
MU11KSUBA:L35556_S_AT eukaryotic translation initiation factor 3, subunit 6 mm
MG-U74AV2:160554_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOE430A:1460432_A_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430A_2:1460432_A_AT eukaryotic translation initiation factor 3, subunit 6 mm
MOUSE430_2:1460432_A_AT eukaryotic translation initiation factor 3, subunit 6 mm
MG-U74AV2:101695_AT Eukaryotic translation initiation factor 3, subunit 6, mRNA (cDNA clone MGC:36795 IMAGE:3988386) mm
MOUSE430A_2:1419822_AT Eukaryotic translation initiation factor 3, subunit 6, mRNA (cDNA clone MGC:36795 IMAGE:3988386) mm
MOE430A:1419822_AT Eukaryotic translation initiation factor 3, subunit 6, mRNA (cDNA clone MGC:36795 IMAGE:3988386) mm
MOUSE430_2:1419822_AT Eukaryotic translation initiation factor 3, subunit 6, mRNA (cDNA clone MGC:36795 IMAGE:3988386) mm
RG-U34C:RC_AI237620_AT eukaryotic translation initiation factor 3, subunit 6 (predicted) rn
RAE230A:1371973_AT eukaryotic translation initiation factor 3, subunit 6 (predicted) rn
RAT230_2:1371973_AT eukaryotic translation initiation factor 3, subunit 6 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC17647 Eukaryotic initiation factor protein 3.E [Caenorhabditis elegans] ref|NP_492785.1| Eukaryotic Initiation Factor family member (eif-3.E) [Caenorhabditis elegans] sp|O61820|IF36_CAEEL Probable eukaryotic translation initiation factor 3 subunit 6 (eIF-3 p48) (eIF3e) 0.0
blast CAE67050 Hypothetical protein CBG12457 [Caenorhabditis briggsae] 1.0E-177
blast XP_686977 PREDICTED: similar to eukaryotic translation initiation factor 3, subunit 6 isoform 1 [Danio rerio] 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 5.5E-25
Sequence
Target Sequence 
>C. ELEGANS:173539_AT
gtcaaggcgacttaggctttacaagttcttccggttttgaatgttttttcctttttcttc
atctgatgctgttctgaaaattgagatgtggcaaacacggagattaagactgtcatctct
cagttttcagtattatttttctttcttatttgttcagctcctcggttttttacgaggact
actcgaattctgtcttttctaaacttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAAGGCGACTTAGGCTTTACAAG 311 463 1298 Antisense
GACTTAGGCTTTACAAGTTCTTCCG 394 371 1306 Antisense
TTACAAGTTCTTCCGGTTTTGAATG 335 667 1316 Antisense
TTTCCTTTTTCTTCATCTGATGCTG 5 671 1344 Antisense
TCTTCATCTGATGCTGTTCTGAAAA 83 617 1353 Antisense
TGTGGCAAACACGGAGATTAAGACT 588 551 1384 Antisense
GATTAAGACTGTCATCTCTCAGTTT 175 431 1399 Antisense
GTCATCTCTCAGTTTTCAGTATTAT 669 467 1409 Antisense
GTTCAGCTCCTCGGTTTTTTACGAG 368 443 1449 Antisense
GAGGACTACTCGAATTCTGTCTTTT 143 405 1471 Antisense
TCGAATTCTGTCTTTTCTAAACTTT 416 601 1480 Antisense
Sequence Source Affymetrix Proprietary Database