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[173538_s_at]

Description C44583 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=M02D8.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C44583 /WB_GENE_ID=M02D8.3 /WP=CE04759 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk371b3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 55      4grp_high_8_8 > 20      6grp_highest_nonorm > 90      6grp_total > 56      4grp_total > 20      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 137.9
(0.8)
P 		158.2
(2.9)
P 		102.4
(-3)
P 		159
(3)
P 		57
B 148.2
(2.3)
P 		119.7
(-3)
P 		119.8
(-3)
P 		151.7
(3)
P 		32
C 115.5
(-2.9)
P 		115
(-3)
P 		117.9
(-2.2)
P 		136.7
(3)
P 		22
avr 133.9
(0.4) 		131
(-0) 		113.4
(-3) 		149.1
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 152.4
(-0.3)
P 		147.5
(-0.8)
P 		125.7
(-2.8)
P 		154.3
(-0.1)
P 		124
(-3)
P 		186.7
(3)
P 		63
B 142.3
(-1.2)
P 		146.4
(-0.9)
P 		161.8
(0.2)
P 		119
(-3)
P 		121.8
(-2.8)
P 		198.8
(3)
P 		80
C 171.4
(2.1)
P 		137.5
(-0.4)
P 		172.6
(2.2)
P 		140.3
(-0.2)
P 		102.5
(-3)
P 		182.8
(3)
P 		80
avr 155.4
(0.2) 		143.8
(-0.7) 		153.4
(0.1) 		137.9
(-1.2) 		116.1
(-3) 		189.4
(3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 50.2
(3)
P 		34.3
(-0.5)
P 		26.8
(-2.2)
P 		23.3
(-3)
P 		27
B 47.3
(3)
P 		29.6
(-2.4)
P 		27.8
(-3)
P 		32.3
(-1.6)
P 		20
C 38.2
(2)
P 		33.1
(0.3)
P 		41.1
(3)
P 		23
(-3)
P 		18
avr 45.2
(3) 		32.3
(-1.1) 		31.9
(-1.2) 		26.2
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 28.1
(-2.8)
P 		52.5
(3)
P 		27.4
(-3)
P 		39.1
(-0.2)
P 		37.4
(-0.6)
P 		45.5
(1.3)
P 		25
B 49.5
(-0.5)
P 		48.7
(-0.6)
P 		39.3
(-1.9)
P 		31.5
(-3)
P 		49.5
(-0.5)
P 		74.1
(3)
P 		43
C 63.5
(3)
P 		41.9
(-0.7)
P 		43.9
(-0.3)
P 		28.2
(-3)
P 		33.2
(-2.2)
P 		61.2
(2.6)
P 		35
avr 47
(0.1) 		47.7
(0.2) 		36.9
(-2.1) 		32.9
(-3) 		40
(-1.4) 		60.3
(3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173538_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25778
Sequence Type Exemplar sequence
Representative Public ID C44583 NCBI
Target Description C44583 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=M02D8.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C44583 /WB_GENE_ID=M02D8.3 /WP=CE04759 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk371b3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077080(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077080 NCBI Caenorhabditis elegans M02D8.3 (M02D8.3) mRNA, complete cds. 11/11 None
SNAP00000039675 ENSEMBL cdna:SNAP chromosome:CEL140:X:8733140:8736944:-1 11/11 None
GENEFINDER00000039690 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8733140:8736944:-1 11/11 None
M02D8.3 ENSEMBL cdna:known chromosome:CEL140:X:8732958:8736944:-1 gene:M02D8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8732967-8736944(-) 97.02 100.0
Public Domain and Genome References
Gene Title
Ensembl M02D8.3
Entrez Gene 181120 Entrez gene
SwissProt Q21461 EMBL-EBI
WormBase CE04759 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509481 M02D8.3 [Caenorhabditis elegans] gb|AAA82379.1| Hypothetical protein M02D8.3 [Caenorhabditis elegans] 0.0
blast CAE69351 Hypothetical protein CBG15437 [Caenorhabditis briggsae] 4.0E-47
blast CAE69351 Hypothetical protein CBG15437 [Caenorhabditis briggsae] 1.0E-44
blast CAE69916 Hypothetical protein CBG16274 [Caenorhabditis briggsae] 2.0E-18
Sequence
Target Sequence 
>C. ELEGANS:173538_S_AT
gattctactcatgttccacgtgaagagctaattgatttggctcgtgagctctttgctatt
ctgcttgacgaatgggctacaagcttcgtgacagaagaggaactagaagctctctataga
cttcaactcgcatcattaagacttgaatgttactcggttacattgaaagattcttttcca
ttttggaaacaagcgaatccggtgggtcttattgagcagacgaatcagtcagagaaaaca
gccgagagaagaaggtcacgtagaaagactcgaaatgcgaatcgaagtttacttgacacc
agtatctgccaaaatgaacaaccaacttcgtctggacgtcgtagacatcactcattctct
gagttcaccgattgtgcaaaaattacaaagaagccaccatcaccttggcccggagcgagg
tttgatgtagaatccccttacggcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTACTCATGTTCCACGTGAAG 180 431 2306 Antisense
TGAGCTCTTTGCTATTCTGCTTGAC 378 569 2350 Antisense
GAATGGGCTACAAGCTTCGTGACAG 313 333 2375 Antisense
GCTCTCTATAGACTTCAACTCGCAT 234 301 2414 Antisense
GAATCCGGTGGGTCTTATTGAGCAG 410 329 2500 Antisense
GAACAACCAACTTCGTCTGGACGTC 192 353 2621 Antisense
GTCGTAGACATCACTCATTCTCTGA 205 473 2643 Antisense
CATTCTCTGAGTTCACCGATTGTGC 180 217 2658 Antisense
CAAAGAAGCCACCATCACCTTGGCC 413 191 2691 Antisense
CTTGGCCCGGAGCGAGGTTTGATGT 425 217 2709 Antisense
TTGATGTAGAATCCCCTTACGGCGA 560 703 2727 Antisense
Sequence Source Affymetrix Proprietary Database