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[173535_s_at]

Description CEC9801 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F33A8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C08980 /WB_GENE_ID=F33A8.5 /WP=CE17757 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk142h7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 51      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 25      6grp_total > 52      4grp_total > 60      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4683
1 2 3 4 range
A 4673.4
(1.4)
P 		5914.7
(3)
P 		4918.5
(1.7)
P 		1288
(-3)
P 		4627
B 5071.9
(2.8)
P 		4824.2
(2.4)
P 		5182.6
(3)
P 		1288.7
(-3)
P 		3894
C 4005.8
(1.8)
P 		4560.3
(2.7)
P 		4718.4
(3)
P 		1232.2
(-3)
P 		3486
avr 4583.7
(2.2) 		5099.7
(3) 		4939.8
(2.7) 		1269.6
(-3) 		3830

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3814
1 2 3 4 5 6 range
A 3580.1
(-2.7)
P 		4940.5
(-0.3)
P 		6850
(3)
P 		5290.6
(0.3)
P 		3698.4
(-2.5)
P 		3428.3
(-3)
P 		3422
B 3932.7
(-1.8)
P 		4269.1
(-1.3)
P 		6996
(3)
P 		5342.9
(0.4)
P 		4140.1
(-1.5)
P 		3182
(-3)
P 		3814
C 4633.3
(-0.3)
P 		4058.8
(-1.4)
P 		6413.1
(3)
P 		4991.1
(0.3)
P 		4299.2
(-1)
P 		3221.5
(-3)
P 		3192
avr 4048.7
(-1.7) 		4422.8
(-1) 		6753
(3) 		5208.2
(0.3) 		4045.9
(-1.7) 		3277.3
(-3) 		3476
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1513
1 2 3 4 range
A 1701.6
(3)
P 		1283.9
(1.3)
P 		1285.8
(1.4)
P 		188.5
(-3)
P 		1513
B 1617.4
(3)
P 		1191.5
(1.1)
P 		1200.9
(1.1)
P 		274.6
(-3)
P 		1343
C 1326
(1.7)
P 		1311.1
(1.6)
P 		1644.5
(3)
P 		207.8
(-3)
P 		1437
avr 1548.3
(3) 		1262.2
(1.7) 		1377.1
(2.2) 		223.6
(-3) 		1325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1097
1 2 3 4 5 6 range
A 660
(-3)
P 		1757.1
(3)
P 		1491.8
(1.5)
P 		1341.8
(0.7)
P 		1116
(-0.5)
P 		834.7
(-2)
P 		1097
B 1368.4
(-0.9)
P 		1421
(-0.3)
P 		1698.8
(3)
P 		1415.1
(-0.3)
P 		1682.1
(2.8)
P 		1186.6
(-3)
P 		512
C 1717.4
(3)
P 		1238.4
(-1)
P 		1632.2
(2.3)
P 		1003.7
(-3)
P 		1391.2
(0.3)
P 		1078.4
(-2.4)
P 		714
avr 1248.6
(-0.8) 		1472.2
(1.6) 		1607.6
(3) 		1253.5
(-0.7) 		1396.4
(0.8) 		1033.2
(-3) 		574
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173535_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23233
Sequence Type Exemplar sequence
Representative Public ID CEC9801 NCBI
Target Description CEC9801 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F33A8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C08980 /WB_GENE_ID=F33A8.5 /WP=CE17757 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk142h7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063968(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063968 NCBI Caenorhabditis elegans F33A8.5 (F33A8.5) mRNA, complete cds. 11/11 None
SNAP00000001855 ENSEMBL cdna:SNAP chromosome:CEL140:II:11049222:11049826:-1 11/11 None
GENEFINDER00000001871 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11049222:11049825:-1 11/11 None
F33A8.5.1 ENSEMBL cdna:known chromosome:CEL140:II:11049019:11049826:-1 gene:F33A8.5 11/11 None
F33A8.5.2 ENSEMBL cdna:known chromosome:CEL140:II:11049019:11049828:-1 gene:F33A8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11049110-11049823(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F33A8.5
Entrez Gene 174692 Entrez gene
SwissProt O62215 EMBL-EBI
WormBase CE17757 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6099 tricarboxylic acid cycle inferred from electronic annotation QuickGO AmiGO
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5740 mitochondrial membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04259 Hypothetical protein F33A8.5 [Caenorhabditis elegans] ref|NP_496369.1| F33A8.5 [Caenorhabditis elegans] sp|O62215|DHSD_CAEEL Putative succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial precursor (CybS) (Succinate-ubiquinone reductase membrane anchor subunit) 2.0E-71
blast CAE59578 Hypothetical protein CBG02978 [Caenorhabditis briggsae] 3.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR007992 EMBL-EBI CybS 1.0E-75
Pfam IPR007992 EMBL-EBI CybS 1.0E-75
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496369.1 2 75-94,109-128
F33A8.5.1 2 75-94,109-128
F33A8.5.2 2 75-94,109-128
SNAP00000001855 2 76-95,110-129
GENEFINDER00000001871 2 75-94,109-128
Sequence
Target Sequence 
>C. ELEGANS:173535_S_AT
atggctcacttccagaaggcacttctcgtcgccagaagcgctccaagaatctcaacaatc
gttagagccacctctactttaaacgatggagccagcaaagttccagaccactctatgcac
tttaagcttgaacgtctttgggccgttggaatgctcccaattcttccagcatcctacttc
atccacggaccagttatggatgccgttctcactgttgccctcactctccatattcattgg
ggaatccacggagtcgtgtacgattacgctcgtccatacgtgatcggagaggctgctgca
aaggccgctcacgtcggagtctacctcatcactggtcttctgctcggcgcactcctccac
ttcaacaccaacgacgtcggaatcaccaaggcctttgaactagtcttctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTCACTTCCAGAAGGCACTTC 466 53 35 Antisense
TCGTCGCCAGAAGCGCTCCAAGAAT 102 603 60 Antisense
GAGCCACCTCTACTTTAAACGATGG 618 383 99 Antisense
GCAAAGTTCCAGACCACTCTATGCA 689 317 129 Antisense
GTCTTTGGGCCGTTGGAATGCTCCC 183 469 168 Antisense
CCCTCACTCTCCATATTCATTGGGG 29 261 252 Antisense
GAGTCGTGTACGATTACGCTCGTCC 112 401 285 Antisense
ACGCTCGTCCATACGTGATCGGAGA 379 91 300 Antisense
CGGAGAGGCTGCTGCAAAGGCCGCT 364 243 319 Antisense
CAACGACGTCGGAATCACCAAGGCC 246 187 403 Antisense
CAAGGCCTTTGAACTAGTCTTCTCC 197 181 421 Antisense
Sequence Source Affymetrix Proprietary Database