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[173528_at]

Description C70369 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=ZK863.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C70369 /WB_GENE_ID=ZK863.4 /WP=CE23469 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk386f12 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 28      4grp_high_8_8 > 0      6grp_highest_nonorm > 96      6grp_total > 77      4grp_total > 07      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 293
1 2 3 4 range
A 610.3
(1.6)
P 		385.9
(-3)
P 		604.9
(1.5)
P 		678.9
(3)
P 		293
B 496.6
(-1)
P 		465.4
(-2.6)
P 		457.2
(-3)
P 		574.2
(3)
P 		117
C 595.8
(2.2)
P 		388.3
(-3)
P 		629.2
(3)
P 		512.8
(0.1)
P 		241
avr 567.6
(2.3) 		413.2
(-3) 		563.8
(2.2) 		588.6
(3) 		175

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1311
1 2 3 4 5 6 range
A 465.2
(-3)
P 		556.1
(-2.3)
P 		615
(-1.8)
P 		710
(-1)
P 		922.9
(0.7)
P 		1208.1
(3)
P 		743
B 642.1
(-2.4)
P 		694.2
(-2.1)
P 		507
(-3)
P 		854.7
(-1.4)
P 		876
(-1.3)
P 		1776.2
(3)
P 		1269
C 593.4
(-2.3)
P 		791.7
(-1.1)
P 		482.7
(-3)
P 		638.5
(-2)
P 		741.1
(-1.4)
P 		1447.1
(3)
P 		964
avr 566.9
(-2.8) 		680.7
(-2.1) 		534.9
(-3) 		734.4
(-1.7) 		846.7
(-1) 		1477.1
(3) 		942
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 222.2
(3)
P 		83.8
(-3)
P 		158.1
(0.2)
P 		99.4
(-2.3)
P 		138
B 158.4
(3)
P 		114.9
(-2)
P 		105.9
(-3)
P 		122.4
(-1.1)
P 		53
C 197.2
(2)
P 		111.7
(-1.9)
P 		219.3
(3)
P 		86.5
(-3)
P 		133
avr 192.6
(3) 		103.5
(-3) 		161.1
(0.9) 		102.8
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 577
1 2 3 4 5 6 range
A 85.8
(-3)
P 		197.8
(0.2)
P 		133.9
(-1.6)
P 		180.1
(-0.3)
P 		278.5
(2.6)
P 		294.1
(3)
P 		208
B 223.4
(-1.9)
P 		231.1
(-1.8)
P 		123.1
(-3)
P 		226.4
(-1.9)
P 		355.9
(-0.4)
P 		662.3
(3)
P 		539
C 219.9
(-1.4)
P 		241.6
(-1)
P 		122.9
(-3)
P 		128.4
(-2.9)
P 		239.8
(-1.1)
P 		484.4
(3)
P 		362
avr 176.4
(-2.2) 		223.5
(-1.4) 		126.6
(-3) 		178.3
(-2.1) 		291.4
(-0.2) 		480.3
(3) 		354
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173528_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25528
Sequence Type Exemplar sequence
Representative Public ID C70369 NCBI
Target Description C70369 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=ZK863.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C70369 /WB_GENE_ID=ZK863.4 /WP=CE23469 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk386f12 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073655(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073655 NCBI Caenorhabditis elegans ZK863.4 (ZK863.4) mRNA, complete cds. 11/11 None
ZK863.4.1 ENSEMBL cdna:known chromosome:CEL140:V:12184100:12186773:-1 gene:ZK863.4 11/11 None
ZK863.4.2 ENSEMBL cdna:known chromosome:CEL140:V:12184110:12186782:-1 gene:ZK863.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12184162-12186764(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK863.4
Entrez Gene 179670 Entrez gene
SwissProt Q23652 EMBL-EBI
WormBase CE23469 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01456 Hypothetical protein ZK863.4 [Caenorhabditis elegans] ref|NP_506056.1| ZK863.4 [Caenorhabditis elegans] 0.0
blast CAE75410 Hypothetical protein CBG23400 [Caenorhabditis briggsae] 0.0
blast CAE61819 Hypothetical protein CBG05789 [Caenorhabditis briggsae] 8.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 1.1E-14
Sequence
Target Sequence 
>C. ELEGANS:173528_AT
ccaacttggccataatcatgttctcaatgtataagtgttctatgaattcgatttgtatgt
atgatccaccatatattttatttcatatttattccggtatttttctgacacgtcacccag
tgaccattttccattgaatctcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAACTTGGCCATAATCATGTTCTC 171 277 1988 Antisense
GGCCATAATCATGTTCTCAATGTAT 304 543 1995 Antisense
GTTCTCAATGTATAAGTGTTCTATG 220 443 2007 Antisense
GTGTTCTATGAATTCGATTTGTATG 278 485 2022 Antisense
GATTTGTATGTATGATCCACCATAT 666 427 2037 Antisense
GATCCACCATATATTTTATTTCATA 670 415 2050 Antisense
TATTCCGGTATTTTTCTGACACGTC 452 663 2077 Antisense
TATTTTTCTGACACGTCACCCAGTG 246 663 2085 Antisense
GACACGTCACCCAGTGACCATTTTC 186 367 2094 Antisense
CACCCAGTGACCATTTTCCATTGAA 309 199 2101 Antisense
GACCATTTTCCATTGAATCTCAACA 563 383 2109 Antisense
Sequence Source Affymetrix Proprietary Database