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[173527_at]

Description CEK003D9F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C16H3.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27759 /WB_GENE_ID=C16H3.3 /WP=CE27697 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk3d9 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 05      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 425.2
(3)
P 		279.2
(-0.6)
P 		239.9
(-1.6)
P 		184.3
(-3)
P 		241
B 398.9
(3)
P 		241.3
(-1.1)
P 		169.2
(-3)
P 		284.3
(0)
P 		230
C 401.6
(3)
P 		266.3
(-0.4)
P 		286.5
(0.1)
P 		165.2
(-3)
P 		236
avr 408.6
(3) 		262.3
(-1.4) 		231.9
(-2.4) 		211.3
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 288
1 2 3 4 5 6 range
A 507.4
(3)
P 		448.4
(1.6)
P 		253.3
(-3)
P 		265
(-2.7)
P 		333.4
(-1.1)
P 		283.3
(-2.3)
P 		254
B 496.6
(3)
P 		409.3
(1.1)
P 		296.6
(-1.4)
P 		301.2
(-1.3)
P 		306.6
(-1.2)
P 		222.6
(-3)
P 		274
C 477.7
(3)
P 		356.4
(0.2)
P 		407.9
(1.4)
P 		402.8
(1.3)
P 		307.2
(-1)
P 		219.5
(-3)
P 		258
avr 493.9
(3) 		404.7
(0.9) 		319.3
(-1.2) 		323
(-1.1) 		315.7
(-1.2) 		241.8
(-3) 		252
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 154.8
(3)
P 		60.6
(-1.4)
P 		62.7
(-1.3)
P 		27
(-3)
P 		128
B 127.2
(3)
P 		59.6
(-1.6)
P 		39.2
(-3)
P 		60.6
(-1.5)
P 		88
C 133
(3)
P 		76.6
(-0.2)
P 		99.8
(1.1)
P 		27.9
(-3)
P 		105
avr 138.3
(3) 		65.6
(-1.4) 		67.2
(-1.3) 		38.5
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 93.5
(-0.8)
P 		159.5
(3)
P 		55.2
(-3)
P 		67.2
(-2.3)
P 		100.6
(-0.4)
P 		69
(-2.2)
P 		104
B 172.8
(3)
P 		136.2
(0.8)
P 		72
(-3)
P 		79.8
(-2.5)
P 		124.6
(0.1)
P 		83
(-2.3)
P 		101
C 177.1
(3)
P 		108.8
(-1)
P 		103.8
(-1.2)
P 		81
(-2.6)
P 		99.4
(-1.5)
P 		73.5
(-3)
P 		104
avr 147.8
(3) 		134.8
(1.9) 		77
(-2.9) 		76
(-2.9) 		108.2
(-0.3) 		75.2
(-3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26095
Sequence Type Exemplar sequence
Representative Public ID CEK003D9F NCBI
Target Description CEK003D9F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C16H3.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27759 /WB_GENE_ID=C16H3.3 /WP=CE27697 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk3d9 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029238(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029238 NCBI Caenorhabditis elegans C16H3.3a (C16H3.3) mRNA, complete cds. 11/11 None
C16H3.3a ENSEMBL cdna:known chromosome:CEL140:X:17601539:17609957:-1 gene:C16H3.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181787 2 1 NM_001029238 173527_at (11), 187800_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17601548-17604453(-) 100.0 100.0
Public Domain and Genome References
Ensembl C16H3.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB07583 Hypothetical protein C16H3.3a [Caenorhabditis elegans] ref|NP_001024409.1| C16H3.3a [Caenorhabditis elegans] 0.0
blast AAO91745 Hypothetical protein C16H3.3b [Caenorhabditis elegans] ref|NP_001024410.1| C16H3.3b [Caenorhabditis elegans] 0.0
blast CAE58698 Hypothetical protein CBG01880 [Caenorhabditis briggsae] 1.0E-101
Sequence
Target Sequence 
>C. ELEGANS:173527_AT
aaccgtgcaatctgaaacttctactctagtagcatcaactaatcaacaatttgtatgaat
aaaaattaatcaaagtcactaaaaatttacttgtcgatttatgtatcaaaaaactatcag
cccaaacaaaaagttgtactctctatatatattttggaatttttaaagtttgtattatat
tgaatgtttttatttttcctgttttcctgtccatggctttattcttgcaattttctccac
ctctctcagttaatctctacttttcaattctgccaaaaacctcctctgaaactcgcaatt
tgtcaatgtttccctatccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCGTGCAATCTGAAACTTCTACT 385 145 1967 Antisense
AACTTCTACTCTAGTAGCATCAACT 674 137 1982 Antisense
GTATCAAAAAACTATCAGCCCAAAC 634 453 2069 Antisense
AAGTTGTACTCTCTATATATATTTT 101 157 2097 Antisense
TGTTTTCCTGTCCATGGCTTTATTC 27 561 2166 Antisense
GCTTTATTCTTGCAATTTTCTCCAC 175 305 2182 Antisense
ACCTCTCTCAGTTAATCTCTACTTT 116 89 2205 Antisense
AATCTCTACTTTTCAATTCTGCCAA 92 171 2218 Antisense
TTCAATTCTGCCAAAAACCTCCTCT 93 685 2229 Antisense
CTCCTCTGAAACTCGCAATTTGTCA 617 233 2247 Antisense
CAATTTGTCAATGTTTCCCTATCCC 699 175 2262 Antisense
Sequence Source Affymetrix Proprietary Database