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[173506_s_at] Mitochondrial complex I 23K chain

Description g4972960 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=T20H4.5 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=T20H4.5 /WP=CE00832 /CHR=3 /FEA=Genomic Cluster /GEN=T20H4.5 /DEF=NADH oxidoreductase complex I 23.8 kDa subunit; Mitochondrial complex I 23K chain
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 16      4grp_high_8_8 > 61      6grp_highest_nonorm > 25      6grp_total > 51      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3980
1 2 3 4 range
A 3698.6
(0.1)
P 		5377.3
(3)
P 		5281.5
(2.8)
P 		1945.5
(-3)
P 		3432
B 3845.7
(0.3)
P 		5115.6
(2.3)
P 		5597.6
(3)
P 		1685.2
(-3)
P 		3912
C 3937.5
(1.1)
P 		4973.6
(3)
P 		4481.2
(2.1)
P 		1618.1
(-3)
P 		3356
avr 3827.3
(0.7) 		5155.5
(3) 		5120.1
(2.9) 		1749.6
(-3) 		3406

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3838
1 2 3 4 5 6 range
A 4334.1
(-0.8)
P 		4770.7
(0.2)
P 		6050.7
(3)
P 		6019.3
(2.9)
P 		3692.2
(-2.2)
P 		3309.1
(-3)
P 		2742
B 4030.2
(0.2)
P 		4493.2
(1.1)
P 		5496.9
(3)
P 		5246.5
(2.5)
P 		4042
(0.2)
P 		2387.9
(-3)
P 		3109
C 3713.9
(-1.4)
P 		4294.8
(-0.3)
P 		6225.6
(3)
P 		5844
(2.3)
P 		3514.5
(-1.7)
P 		2764.7
(-3)
P 		3461
avr 4026.1
(-0.7) 		4519.6
(0.3) 		5924.4
(3) 		5703.3
(2.6) 		3749.6
(-1.2) 		2820.6
(-3) 		3104
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1289
1 2 3 4 range
A 1346.7
(2.8)
P 		1167.3
(1.8)
P 		1380.7
(3)
P 		284.8
(-3)
P 		1096
B 1226.4
(2.5)
P 		1263.4
(2.8)
P 		1297.1
(3)
P 		359.1
(-3)
P 		938
C 1303.4
(1.8)
P 		1429.9
(2.4)
P 		1561.8
(3)
P 		272.8
(-3)
P 		1289
avr 1292.2
(2.3) 		1286.9
(2.3) 		1413.2
(3) 		305.6
(-3) 		1108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 898
1 2 3 4 5 6 range
A 799
(-3)
P 		1696.7
(3)
P 		1317.7
(0.5)
P 		1526.6
(1.9)
P 		1114.1
(-0.9)
P 		805.6
(-3)
P 		898
B 1402.3
(1.1)
P 		1495.6
(1.8)
P 		1334.8
(0.5)
P 		1389.6
(1)
P 		1642.2
(3)
P 		890.4
(-3)
P 		752
C 1376.6
(1.1)
P 		1310.5
(0.5)
P 		1584.5
(3)
P 		1175.2
(-0.7)
P 		1137.2
(-1.1)
P 		925.5
(-3)
P 		659
avr 1192.6
(0.1) 		1500.9
(3) 		1412.3
(2.2) 		1363.8
(1.7) 		1297.8
(1.1) 		873.8
(-3) 		627
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173506_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23891
Sequence Type Exemplar sequence
Representative Public ID 4972960 NCBI
Target Description g4972960 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=T20H4.5 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=T20H4.5 /WP=CE00832 /CHR=3 /FEA=Genomic Cluster /GEN=T20H4.5 /DEF=NADH oxidoreductase complex I 23.8 kDa subunit; Mitochondrial complex I 23K chain
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066194(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066194 NCBI Caenorhabditis elegans T20H4.5 (T20H4.5) mRNA, complete cds. 11/11 None
SNAP00000008519 ENSEMBL cdna:SNAP chromosome:CEL140:III:7233126:7234105:-1 11/11 None
GENEFINDER00000008528 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7233126:7234105:-1 11/11 None
T20H4.5 ENSEMBL cdna:known chromosome:CEL140:III:7232850:7234126:-1 gene:T20H4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7232975-7234104(-) 100.0 100.0
Public Domain and Genome References
Gene Title Mitochondrial complex I 23K chain
Ensembl T20H4.5
Entrez Gene 176023 Entrez gene
SwissProt Q22619 EMBL-EBI
WormBase CE00832 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264097_S_AT NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial, putative at
DROSOPHILA_2:1627220_AT NADH:ubiquinone reductase 23kD subunit precursor dm
DROSGENOME1:153134_AT NADH:ubiquinone reductase 23kD subunit precursor dm
HG-U133_PLUS_2:232169_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133B:232169_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133_PLUS_2:203190_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133A_2:203190_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133A:203190_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-FOCUS:203190_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133A_2:203189_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133A:203189_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U133_PLUS_2:203189_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HU35KSUBA:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HU35KSUBB:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HU35KSUBC:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HU35KSUBD:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HUGENEFL:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HC-G110:HUM_ALU_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HU35KSUBB:RC_R17000_S_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HUGENEFL:U65579_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
U133_X3P:G4505370_3P_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
U133_X3P:HS.90443.1.A1_3P_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U95AV2:38257_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U95C:58954_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
HG-U95E:79643_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) hs
MOUSE430A_2:1455283_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1455283_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1455283_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430A_2:1434213_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1434213_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1434213_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MG-U74CV2:165405_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1423907_A_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430A_2:1423907_A_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1423907_A_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1434212_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430A_2:1434212_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1434212_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1434579_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430A_2:1434579_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1434579_X_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOE430A:1423908_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430A_2:1423908_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MOUSE430_2:1423908_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 mm
MU19KSUBC:TC40892_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (Ndufs8), mRNA mm
RT-U34:RC_AA799479_G_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RG-U34A:RC_AA799479_G_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RT-U34:RC_AA799479_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RG-U34A:RC_AA799479_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RT-U34:AA685112_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RG-U34A:AA685112_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RT-U34:RC_AA851379_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RG-U34A:RC_AA851379_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RAE230A:1388326_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
RAT230_2:1388326_AT NADH dehydrogenase (ubiquinone) Fe-S protein 8 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
5506 iron ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA50662 Hypothetical protein T20H4.5 [Caenorhabditis elegans] ref|NP_498595.1| T20H4.5 [Caenorhabditis elegans] gb|AAD34863.1| NADH oxidoreductase complex I 23.8 kDa subunit [Caenorhabditis elegans] sp|Q22619|NUIM_CAEEL NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial precursor (Complex I-23KD) (CI-23KD) 1.0E-113
blast CAE56449 Hypothetical protein CBG24154 [Caenorhabditis briggsae] 1.0E-111
blast EAL28402 GA17794-PA [Drosophila pseudoobscura] 4.0E-69
Protein Domains
Database ID Description E-Value
scop a.1.2.Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain All alpha proteins; Globin-like; alpha-helical ferredoxin; Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain 5.30000019073486
Pfam IPR001450 EMBL-EBI 4Fe-4S ferredoxin, iron-sulfur binding domain 4.9E-7
Pfam IPR001450 EMBL-EBI 4Fe-4S ferredoxin, iron-sulfur binding domain 8.8E-11
Sequence
Target Sequence 
>C. ELEGANS:173506_S_AT
atgttttcttcaccgaacttttcagaggtttcggagtgatgcttggtcacgtattcatgg
agccagctactatcaactatcccttcgaaaagggaccactcagttcaagattcagaggag
aacacgctcttcgtcgatacccgtctggtgaagaaagatgcattgcttgcaagctttgcg
aagccatttgcccagctcaagctatcacgatcgaggccgaaacaagacctgacggaagtc
gccgtaccactagatacgatatcgatatgaccaagtgcatctattgtggattatgccagg
aagcttgcccagtcgatgcaattgtcgagggcccgaacttcgaatattcgactgaaactc
acgaagaactcctctataataaggagaagctcttgttgaatggtgatcgctgggagcctg
agctcgcttccaatcttcaagccgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTTTCTTCACCGAACTTTTCAG 162 45 192 Antisense
GGAGCCAGCTACTATCAACTATCCC 31 523 250 Antisense
AGAGGAGAACACGCTCTTCGTCGAT 685 65 305 Antisense
TTCGTCGATACCCGTCTGGTGAAGA 263 695 321 Antisense
AAGCTTTGCGAAGCCATTTGCCCAG 299 147 362 Antisense
ATTTGCCCAGCTCAAGCTATCACGA 548 17 377 Antisense
GACGGAAGTCGCCGTACCACTAGAT 199 375 422 Antisense
ATTATGCCAGGAAGCTTGCCCAGTC 349 13 481 Antisense
TGCAATTGTCGAGGGCCCGAACTTC 521 553 508 Antisense
GAAACTCACGAAGAACTCCTCTATA 403 355 545 Antisense
CTCGCTTCCAATCTTCAAGCCGAAT 481 233 614 Antisense
Sequence Source GenBank