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[173487_at]

Description C42813 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R04D3.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C42813 /WB_GENE_ID=R04D3.2 /WP=CE06223 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk319h6 : 5prime end, single read.
Cluster 6grp_total > 88      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 0.8
(-3)
A 		2.8
(-2.4)
A 		9.7
(-0.5)
A 		21.9
(3)
A 		21
B 10.9
(-1.9)
A 		3
(-3)
A 		4.4
(-2.8)
A 		46
(3)
A 		43
C 2
(-2.9)
A 		2
(-2.9)
A 		1.5
(-3)
A 		52.9
(3)
A 		51
avr 4.6
(-2.7) 		2.6
(-3) 		5.2
(-2.6) 		40.3
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 3.3
(-2.9)
A 		1.3
(-3)
A 		19.6
(-1.9)
A 		1.4
(-3)
A 		94.6
(2.6)
P 		101.5
(3)
P 		100
B 10.5
(-2.7)
A 		20.5
(-2.3)
A 		16
(-2.5)
A 		2
(-3)
A 		72.6
(-0.3)
P 		161.5
(3)
P 		160
C 3.2
(-2.9)
A 		2.4
(-3)
A 		1.4
(-3)
A 		26.7
(-2.3)
A 		72.4
(-1)
P 		210.8
(3)
P 		209
avr 5.7
(-3) 		8.1
(-2.9) 		12.3
(-2.7) 		10
(-2.8) 		79.9
(-0.1) 		157.9
(3) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 0.3
(-3)
A 		0.6
(-2.4)
A 		2.5
(1.6)
A 		3.2
(3)
A 		3
B 3.5
(-1.2)
A 		0.7
(-3)
A 		1
(-2.8)
A 		9.8
(3)
A 		9
C 0.7
(-2.9)
A 		0.6
(-2.9)
A 		0.5
(-3)
A 		8.9
(3)
A 		8
avr 1.5
(-2.2) 		0.6
(-3) 		1.3
(-2.4) 		7.3
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 0.6
(-3)
A 		0.4
(-3)
A 		4.3
(-2.2)
A 		0.4
(-3)
A 		28.5
(3)
P 		24.7
(2.2)
P 		28
B 3.7
(-2.7)
A 		6.8
(-2.4)
A 		3.9
(-2.7)
A 		0.5
(-3)
A 		29.5
(-0.1)
P 		60.2
(3)
P 		60
C 1.2
(-2.9)
A 		0.7
(-3)
A 		0.3
(-3)
A 		5.4
(-2.6)
A 		23.4
(-1)
P 		70.6
(3)
P 		70
avr 1.8
(-3) 		2.6
(-2.9) 		2.8
(-2.9) 		2.1
(-3) 		27.1
(0) 		51.8
(3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173487_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26124
Sequence Type Exemplar sequence
Representative Public ID C42813 NCBI
Target Description C42813 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R04D3.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C42813 /WB_GENE_ID=R04D3.2 /WP=CE06223 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk319h6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077807 NCBI Caenorhabditis elegans R04D3.2 (R04D3.2) mRNA, complete cds. 11/11 None
R04D3.2 ENSEMBL cdna:known chromosome:CEL140:X:13285060:13286553:-1 gene:R04D3.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13285074-13286551(-) 99.7 99.7
Public Domain and Genome References
Gene Title
Ensembl R04D3.2
Entrez Gene 181451 Entrez gene
SwissProt Q21712 EMBL-EBI
WormBase CE06223 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94162 Hypothetical protein R04D3.2 [Caenorhabditis elegans] ref|NP_510208.1| R04D3.2 [Caenorhabditis elegans] 1.0E-177
blast CAA94163 Hypothetical protein R04D3.3 [Caenorhabditis elegans] ref|NP_510210.1| R04D3.3 [Caenorhabditis elegans] 2.0E-88
blast CAE61408 Hypothetical protein CBG05271 [Caenorhabditis briggsae] 3.0E-69
Sequence
Target Sequence 
>C. ELEGANS:173487_AT
gtccttatgttctgaaactctcctggatttttctagattcttttccagttacatttcaca
tagaatctctaactaccggtgcatttgccaatct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCTTATGTTCTGAAACTCTCCTG 148 477 1220 Antisense
TGTTCTGAAACTCTCCTGGATTTTT 589 561 1227 Antisense
AACTCTCCTGGATTTTTCTAGATTC 524 141 1235 Antisense
TCCTGGATTTTTCTAGATTCTTTTC 183 595 1240 Antisense
TCTAGATTCTTTTCCAGTTACATTT 574 613 1251 Antisense
GATTCTTTTCCAGTTACATTTCACA 78 431 1255 Antisense
CTTTTCCAGTTACATTTCACATAGA 163 221 1259 Antisense
GTTACATTTCACATAGAATCTCTAA 96 447 1267 Antisense
AGAATCTCTAACTACCGGTGCATTT 124 75 1281 Antisense
CTCTAACTACCGGTGCATTTGCCAA 501 227 1286 Antisense
TAACTACCGGTGCATTTGCCAATCT 439 635 1289 Antisense
Sequence Source Affymetrix Proprietary Database