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[173486_at]

Description C45424 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F31C3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C45424 /WB_GENE_ID=F31C3.4 /WP=CE17733 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393g5 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_highest_8_8 > 28      4grp_high_8_8 > 31      6grp_highest_nonorm > 93      6grp_total > 98      4grp_total > 0      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 252.9
(1.5)
P 		141.7
(-3)
P 		291.2
(3)
P 		256
(1.6)
P 		150
B 224.6
(-2.4)
P 		218.2
(-3)
P 		288
(3)
P 		259
(0.5)
P 		70
C 241.6
(-1.4)
P 		203.5
(-3)
P 		344.6
(3)
P 		239.8
(-1.5)
P 		141
avr 239.7
(-0.4) 		187.8
(-3) 		307.9
(3) 		251.6
(0.2) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 504
1 2 3 4 5 6 range
A 210.9
(-3)
P 		288.9
(-1.6)
P 		221.6
(-2.8)
P 		317.7
(-1.1)
P 		521.6
(2.7)
P 		540.4
(3)
P 		330
B 195.3
(-3)
P 		233.5
(-2.5)
P 		215.2
(-2.8)
P 		279.3
(-2)
P 		545.1
(1.2)
P 		699.1
(3)
P 		504
C 225.3
(-3)
P 		245.7
(-2.6)
P 		277.9
(-2.1)
P 		260.3
(-2.4)
P 		424.7
(0.6)
P 		559
(3)
P 		334
avr 210.5
(-3) 		256
(-2.3) 		238.2
(-2.6) 		285.8
(-1.8) 		497.1
(1.4) 		599.5
(3) 		389
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 92.1
(3)
P 		30.8
(-3)
P 		76.1
(1.4)
P 		37.5
(-2.3)
P 		61
B 71.6
(3)
P 		53.9
(-3)
P 		66.7
(1.3)
P 		55.2
(-2.6)
P 		18
C 80
(-0)
P 		58.5
(-1.6)
P 		120.1
(3)
P 		40.4
(-3)
P 		80
avr 81.2
(2.1) 		47.7
(-2.5) 		87.6
(3) 		44.4
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 38.9
(-3)
P 		102.8
(0.2)
P 		48.3
(-2.5)
P 		80.6
(-0.9)
P 		157.4
(3)
P 		131.6
(1.7)
P 		119
B 67.9
(-2.5)
P 		77.7
(-2.3)
P 		52.2
(-3)
P 		74
(-2.4)
P 		221.5
(1.9)
P 		260.7
(3)
P 		209
C 83.5
(-1.6)
P 		75
(-2)
P 		70.7
(-2.2)
P 		52.3
(-3)
P 		137.4
(0.8)
P 		187.1
(3)
P 		135
avr 63.4
(-2.7) 		85.2
(-1.8) 		57.1
(-3) 		69
(-2.5) 		172.1
(2.1) 		193.1
(3) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173486_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22683
Sequence Type Exemplar sequence
Representative Public ID C45424 NCBI
Target Description C45424 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F31C3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C45424 /WB_GENE_ID=F31C3.4 /WP=CE17733 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk393g5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061225 NCBI Caenorhabditis elegans F31C3.4 (F31C3.4) mRNA, complete cds. 11/11 None
F31C3.4 ENSEMBL cdna:known chromosome:CEL140:I:15058983:15060538:-1 gene:F31C3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:15058924-15060470(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31C3.4
Entrez Gene 173376 Entrez gene
SwissProt O62192 EMBL-EBI
WormBase CE17733 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.26608.1.S1_AT similar to solute carrier family 39 (zinc transporter), member 1 cfa
DROSOPHILA_2:1635384_AT Zinc/iron regulated transporter-related protein 1 dm
DROSGENOME1:146688_AT Zinc/iron regulated transporter-related protein 1 dm
HG-U133_PLUS_2:217778_AT solute carrier family 39 (zinc transporter), member 1 hs
HG-FOCUS:217778_AT solute carrier family 39 (zinc transporter), member 1 hs
HG-U133A_2:217778_AT solute carrier family 39 (zinc transporter), member 1 hs
HG-U133A:217778_AT solute carrier family 39 (zinc transporter), member 1 hs
HU35KSUBA:RC_AA159025_AT solute carrier family 39 (zinc transporter), member 1 hs
U133_X3P:G7657700_3P_AT solute carrier family 39 (zinc transporter), member 1 hs
HG-U95B:46281_AT solute carrier family 39 (zinc transporter), member 1 hs
HG-U95D:81223_AT Solute carrier family 39 (zinc transporter), member 1 hs
MG-U74AV2:96832_AT solute carrier family 39 (zinc transporter), member 1 mm
MOUSE430_2:1424424_AT solute carrier family 39 (zinc transporter), member 1 mm
MOE430A:1424424_AT solute carrier family 39 (zinc transporter), member 1 mm
MOUSE430A_2:1424424_AT solute carrier family 39 (zinc transporter), member 1 mm
MG-U74AV2:100373_AT solute carrier family 39 (zinc transporter), member 1 mm
MU19KSUBB:TC26882_S_AT Solute carrier family 39 (zinc transporter), member 1, mRNA (cDNA clone MGC:6744 IMAGE:3591856) mm
MU19KSUBB:TC33328_AT Solute carrier family 39 (zinc transporter), member 1, mRNA (cDNA clone MGC:6744 IMAGE:3591856) mm
RAE230A:1372778_AT Solute carrier family 39 (zinc transporter), member 1 (predicted) rn
RAT230_2:1372778_AT Solute carrier family 39 (zinc transporter), member 1 (predicted) rn
RG-U34C:AA799420_AT Solute carrier family 39 (zinc transporter), member 1 (predicted) rn
RG-U34C:RC_AI112161_AT Solute carrier family 39 (zinc transporter), member 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30001 metal ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46873 metal ion transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07194 Hypothetical protein F31C3.4 [Caenorhabditis elegans] ref|NP_493626.1| F31C3.4 [Caenorhabditis elegans] 1.0E-156
blast CAE72554 Hypothetical protein CBG19738 [Caenorhabditis briggsae] 1.0E-137
blast CAF99867 unnamed protein product [Tetraodon nigroviridis] 2.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 2.7E-92
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493626.1 8 32-54,63-85,100-117,165-187,197-219,226-248,258-280,292-314
F31C3.4 8 32-54,63-85,100-117,165-187,197-219,226-248,258-280,292-314
Sequence
Target Sequence 
>C. ELEGANS:173486_AT
gaagcttcttaggacatttgtatttctttttctccaggaattcgattaatttttgacatt
ttgtagcattttctctgctttagtttttttttcctaattctgtgcaattttactcgattt
ttgtgtacattctgtgtcccatcttttcgctatcacacattttcttgtactttctcatcc
cgggttccagctcgccatagacctgtgtatttcattcattttcccaattccattactta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTTCTTAGGACATTTGTATTT 112 343 959 Antisense
GTATTTCTTTTTCTCCAGGAATTCG 201 451 978 Antisense
GTAGCATTTTCTCTGCTTTAGTTTT 174 457 1021 Antisense
TTTTTTTCCTAATTCTGTGCAATTT 334 675 1044 Antisense
TGTGCAATTTTACTCGATTTTTGTG 297 557 1059 Antisense
TTTTGTGTACATTCTGTGTCCCATC 179 679 1077 Antisense
CTTTTCGCTATCACACATTTTCTTG 330 223 1101 Antisense
TCACACATTTTCTTGTACTTTCTCA 284 629 1111 Antisense
CAGCTCGCCATAGACCTGTGTATTT 705 203 1146 Antisense
TAGACCTGTGTATTTCATTCATTTT 305 653 1156 Antisense
TTCATTTTCCCAATTCCATTACTTA 595 687 1173 Antisense
Sequence Source Affymetrix Proprietary Database