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[173477_at]

Description g8037940 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F35G12.3A /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F35G12.3A /WP=CE00971 /CHR=3 /FEA=Genomic Cluster /DEF=SEL-5A serine-threonine kinase; serine-threonine kinase
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 09      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 65      6grp_total > 90      4grp_total > 06      6grp_highest_sub > 05      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 798
1 2 3 4 range
A 663.7
(-1.9)
P 		614.8
(-2.3)
P 		528.5
(-3)
P 		1272.6
(3)
P 		744
B 1008.6
(3)
P 		609.6
(-2.6)
P 		581
(-3)
P 		857.9
(0.9)
P 		428
C 962.8
(1.1)
P 		475.1
(-3)
P 		785.3
(-0.4)
P 		1186.3
(3)
P 		711
avr 878.4
(0.5) 		566.5
(-3) 		631.6
(-2.3) 		1105.6
(3) 		539

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1141
1 2 3 4 5 6 range
A 1326.3
(2.3)
P 		1026.8
(-1)
P 		889
(-2.6)
P 		1126
(0.1)
P 		1389
(3)
P 		851.9
(-3)
P 		537
B 985.3
(0.4)
P 		1389
(2.5)
P 		336.6
(-3)
P 		1477.6
(3)
P 		1140.1
(1.2)
P 		1269.8
(1.9)
P 		1141
C 909.4
(-0.7)
P 		962.7
(-0.4)
P 		796
(-1.5)
P 		1463.6
(3)
P 		968.6
(-0.3)
P 		567
(-3)
P 		897
avr 1073.7
(0.5) 		1126.2
(1) 		673.9
(-3) 		1355.7
(3) 		1165.9
(1.3) 		896.2
(-1) 		682
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 241.7
(3)
P 		133.5
(-3)
P 		138.2
(-2.7)
P 		186.3
(-0.1)
P 		108
B 321.6
(3)
P 		150.6
(-2.5)
P 		134.6
(-3)
P 		182.8
(-1.5)
P 		187
C 318.7
(3)
P 		136.6
(-3)
P 		273.7
(1.5)
P 		200
(-0.9)
P 		182
avr 294
(3) 		140.2
(-3) 		182.2
(-1.4) 		189.7
(-1.1) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 244.5
(-1.6)
P 		365.2
(1.6)
P 		193.6
(-3)
P 		285.6
(-0.6)
P 		419.1
(3)
P 		207.4
(-2.6)
P 		226
B 342.8
(1)
P 		462.3
(2.8)
P 		81.7
(-3)
P 		391.4
(1.7)
P 		463.2
(2.8)
P 		473.5
(3)
P 		392
C 337.1
(3)
P 		293.7
(1.2)
P 		202.6
(-2.5)
P 		294.3
(1.3)
P 		313.4
(2)
P 		189.8
(-3)
P 		147
avr 308.1
(0.7) 		373.7
(2.4) 		159.3
(-3) 		323.8
(1.1) 		398.6
(3) 		290.2
(0.3) 		239
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173477_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24353
Sequence Type Exemplar sequence
Representative Public ID 8037940 NCBI
Target Description g8037940 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F35G12.3A /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F35G12.3A /WP=CE00971 /CHR=3 /FEA=Genomic Cluster /DEF=SEL-5A serine-threonine kinase; serine-threonine kinase
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027391(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027391 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-5) (sel-5) mRNA, complete cds. 11/11 None
F35G12.3b ENSEMBL cdna:known chromosome:CEL140:III:4571756:4578402:-1 gene:F35G12.3 11/11 None
F35G12.3a ENSEMBL cdna:known chromosome:CEL140:III:4571780:4578392:-1 gene:F35G12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175599 2 1 NM_001027391 173477_at (11), 190859_s_at (11)
NM_001027392 173478_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4571791-4578393(-) 97.36 100.0
Public Domain and Genome References
Ensembl F35G12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86326 Hypothetical protein F35G12.3a [Caenorhabditis elegans] ref|NP_001022562.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71545.1| SEL-5A serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAE71182 Hypothetical protein CBG18039 [Caenorhabditis briggsae] 0.0
blast CAA86332 Hypothetical protein F35G12.3b [Caenorhabditis elegans] ref|NP_001022563.1| Suppressor/Enhancer of Lin-12 family member (sel-5) [Caenorhabditis elegans] gb|AAF71546.1| SEL-5B serine/threonine kinase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.9E-36
Sequence
Target Sequence 
>C. ELEGANS:173477_AT
cgacttgtaaactggaatttttcatatttctttttatatattacacaatttgttcttttt
agtgatttgcaattcaccctccccccaaagttgtcgttgtgtcaaatttagaatcactta
ttttctcaattatttattttcttttggtattttcccgccaatgtacttatgatatgtata
atatggaatatgctttccttgtaattttcatattttggtatccacccacgcatgtagttg
tgatagactcaatctcccagtttcatatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACTTGTAAACTGGAATTTTTCAT 91 115 3238 Antisense
ACACAATTTGTTCTTTTTAGTGATT 508 111 3280 Antisense
TCCCCCCAAAGTTGTCGTTGTGTCA 193 595 3317 Antisense
TTCTTTTGGTATTTTCCCGCCAATG 614 673 3376 Antisense
TTCCCGCCAATGTACTTATGATATG 115 699 3389 Antisense
GAATATGCTTTCCTTGTAATTTTCA 203 327 3423 Antisense
GCTTTCCTTGTAATTTTCATATTTT 151 305 3429 Antisense
TCATATTTTGGTATCCACCCACGCA 20 619 3445 Antisense
ATCCACCCACGCATGTAGTTGTGAT 582 39 3457 Antisense
GTTGTGATAGACTCAATCTCCCAGT 257 437 3474 Antisense
AGACTCAATCTCCCAGTTTCATATG 625 69 3482 Antisense
Sequence Source GenBank