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[173473_s_at]

Description g4324469 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=C09G4.1 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=C09G4.1 /WP=CE27675 /CHR=4 /FEA=Genomic Cluster /GEN=LAG1-1; hyl-1 /DEF=LAG1Ce-1
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 95      6grp_total > 88      4grp_total > 40      6grp_highest_sub > Da      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 582
1 2 3 4 range
A 420
(-3)
P 		646.2
(-0.1)
P 		895.2
(3)
P 		613.5
(-0.6)
P 		475
B 379.9
(-3)
P 		843
(1.8)
P 		961.7
(3)
P 		946.5
(2.8)
P 		582
C 441.3
(-3)
P 		936.7
(3)
P 		926.4
(2.9)
P 		857.2
(2)
P 		495
avr 413.7
(-3) 		808.6
(1.6) 		927.8
(3) 		805.7
(1.6) 		514

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 435.2
(-3)
P 		566.7
(-1.2)
P 		497.7
(-2.1)
P 		536.5
(-1.6)
P 		780.3
(1.7)
P 		873.3
(3)
P 		438
B 532.2
(-2.5)
P 		569.8
(-2)
P 		493.4
(-3)
P 		653.3
(-0.9)
P 		863.1
(1.9)
P 		944
(3)
P 		451
C 574.4
(-1.4)
P 		574.5
(-1.4)
P 		439.2
(-3)
P 		550.6
(-1.7)
P 		619.9
(-0.9)
P 		950.9
(3)
P 		512
avr 513.9
(-2.5) 		570.3
(-1.7) 		476.8
(-3) 		580.1
(-1.6) 		754.4
(0.7) 		922.7
(3) 		446
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 152.9
(-0.4)
P 		140.3
(-0.9)
P 		234
(3)
P 		89.8
(-3)
P 		144
B 121.1
(-3)
P 		208.2
(2.1)
P 		222.9
(3)
P 		201.7
(1.8)
P 		102
C 146.1
(-2.9)
P 		269.3
(1.2)
P 		322.9
(3)
P 		144.5
(-3)
P 		178
avr 140
(-3) 		205.9
(0.3) 		259.9
(3) 		145.3
(-2.7) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 80.2
(-3)
P 		201.5
(1.7)
P 		108.4
(-1.9)
P 		136.1
(-0.8)
P 		235.5
(3)
P 		212.6
(2.1)
P 		155
B 185.2
(-1.3)
P 		189.7
(-1.2)
P 		119.8
(-3)
P 		173
(-1.6)
P 		350.7
(3)
P 		352
(3)
P 		232
C 212.9
(-0)
P 		175.3
(-1.1)
P 		111.8
(-3)
P 		110.7
(-3)
P 		200.6
(-0.4)
P 		318.3
(3)
P 		208
avr 159.4
(-1.5) 		188.8
(-0.5) 		113.3
(-3) 		139.9
(-2.1) 		262.3
(1.9) 		294.3
(3) 		181
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173473_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24731
Sequence Type Exemplar sequence
Representative Public ID 4324469 NCBI
Target Description g4324469 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=C09G4.1 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=C09G4.1 /WP=CE27675 /CHR=4 /FEA=Genomic Cluster /GEN=LAG1-1; hyl-1 /DEF=LAG1Ce-1
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069058(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069058 NCBI Caenorhabditis elegans Homolog of Yeast Longevity gene family member (hyl-1) (hyl-1) mRNA, complete cds. 11/11 None
SNAP00000025649 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8540645:8544037:1 11/11 None
GENEFINDER00000025651 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8541077:8544528:1 11/11 None
C09G4.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:8540536:8544350:1 gene:C09G4.1 11/11 None
C09G4.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:8540584:8544289:1 gene:C09G4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8540651-8544054(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hyl-1
Ensembl C09G4.1
Entrez Gene 177660 Entrez gene
SwissProt Q9GYR9 EMBL-EBI
WormBase CE27675 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257038_AT longevity-assurance (LAG1) family protein at
CANINE_2:CFAAFFX.13123.1.S1_AT similar to LAG1 longevity assurance homolog 5 cfa
CANINE_2:CFAAFFX.13129.1.S1_AT similar to LAG1 longevity assurance homolog 5 cfa
CANINE_2:CFAAFFX.13129.1.S1_S_AT similar to LAG1 longevity assurance homolog 5 cfa
DROSOPHILA_2:1629286_S_AT Longevity assurance gene 1 dm
DROSGENOME1:151387_AT Longevity assurance gene 1 dm
DROSGENOME1:144584_AT Longevity assurance gene 1 dm
CHICKEN:GGA.16178.1.S1_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) gga
HG-U133_PLUS_2:239491_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133B:239491_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HU35KSUBA:AA236771_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
U133_X3P:HS.5331.0.S1_3P_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
U133_X3P:HS.101368.0.A1_3P_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133_PLUS_2:224951_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133B:224951_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U95B:49885_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U95B:53053_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HU35KSUBA:RC_AA136724_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
U133_X3P:HS2.270525.1.A1_3P_S_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133_PLUS_2:1556783_A_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133_PLUS_2:244437_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U133B:244437_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U95B:48407_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HG-U95E:77114_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
HU35KSUBC:RC_D25645_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
U133_X3P:HS.193969.0.S1_3P_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
U133_X3P:HS.312340.0.A1_3P_AT LAG1 longevity assurance homolog 5 (S. cerevisiae) hs
MG-U74BV2:163109_AT longevity assurance homolog 5 (S. cerevisiae) mm
MOE430A:1451209_AT longevity assurance homolog 5 (S. cerevisiae) mm
MOUSE430A_2:1451209_AT longevity assurance homolog 5 (S. cerevisiae) mm
MOUSE430_2:1451209_AT longevity assurance homolog 5 (S. cerevisiae) mm
MOE430B:1447308_AT longevity assurance homolog 5 (S. cerevisiae) mm
MOUSE430_2:1447308_AT longevity assurance homolog 5 (S. cerevisiae) mm
MU19KSUBC:TC40641_AT Longevity assurance homolog 5 (S. cerevisiae) (Lass5), mRNA mm
RG-U34C:RC_AI228980_AT longevity assurance homolog 5 (S. cerevisiae) (predicted) rn
RAE230A:1371676_AT longevity assurance homolog 5 (S. cerevisiae) (predicted) rn
RAT230_2:1371676_AT longevity assurance homolog 5 (S. cerevisiae) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
46513 ceramide biosynthesis inferred from sequence similarity QuickGO AmiGO
7568 aging inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
5789 endoplasmic reticulum membrane inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99867 Homolog of yeast longevity gene protein 1 [Caenorhabditis elegans] ref|NP_501459.1| Homolog of Yeast Longevity gene family member (hyl-1) [Caenorhabditis elegans] gb|AAD16893.1| LAG1Ce-1 [Caenorhabditis elegans] 0.0
blast T29831 hypothetical protein C09G4.1 - Caenorhabditis elegans 1.0E-177
blast T29831 hypothetical protein C09G4.1 - Caenorhabditis elegans 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR005547 EMBL-EBI Longevity-assurance protein (LAG1) 2.8E-114
Pfam IPR005547 EMBL-EBI Longevity-assurance protein (LAG1) 2.8E-114
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501459.1 5 36-58,96-113,138-160,223-245,275-297
C09G4.1.1 5 36-58,96-113,138-160,223-245,275-297
C09G4.1.2 5 36-58,96-113,138-160,223-245,275-297
SNAP00000025649 5 36-58,96-113,138-160,223-245,275-297
GENEFINDER00000025651 6 33-55,93-110,135-157,220-242,272-294,386-408
Sequence
Target Sequence 
>C. ELEGANS:173473_S_AT
taatggttcatcacgtaatcactatattccttttgtcatccagttggaccattaattttg
ttagggtcggaaccttgattcttctctctcatgatgtttcggatgtattcctagagggag
gaaaacttgttcgatatgatgctcacaacaagaatatgaccaatttcatgtttgtactat
tcttcagttcatgggttgccactcgtctcatctattatccattcattgttattcgctcag
cagttacagaagctgctgcccttattcagccagattacatactttgggattatcaattgg
gcccaccatatgcacctcgcctcatcgttttcgctctcattctccttttcttccttcaca
ttttctggactttcattattcttcgtattgcctatcgtacttcaaccggagggcaggcta
aggacgtcagatcagattctgattcggattatgatgaggaagaaatggcgagacgtgaaa
gaacacgacttcttaagaagaaaaagaataaggggtatctccgagcaccgacgacgatga
tgacgaaggagaagaagagaaaaatgacagaaaagctcgccatcgcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATGGTTCATCACGTAATCACTAT 296 637 514 Antisense
ATCACTATATTCCTTTTGTCATCCA 605 27 531 Antisense
TATTCTTCAGTTCATGGGTTGCCAC 280 661 691 Antisense
TCGTCTCATCTATTATCCATTCATT 357 605 716 Antisense
TCCATTCATTGTTATTCGCTCAGCA 314 591 731 Antisense
TACAGAAGCTGCTGCCCTTATTCAG 638 641 758 Antisense
CCTTCACATTTTCTGGACTTTCATT 473 267 866 Antisense
ATTATTCTTCGTATTGCCTATCGTA 651 13 888 Antisense
GCCTATCGTACTTCAACCGGAGGGC 621 263 903 Antisense
GAGGGCAGGCTAAGGACGTCAGATC 588 401 922 Antisense
ATGACAGAAAAGCTCGCCATCGCAG 25 45 1077 Antisense
Sequence Source GenBank