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[173471_at]

Description g6760364 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=Y71F9B.7 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=Y71F9B.7 /WP=CE22877 /CHR=1 /FEA=Genomic Cluster /GEN=plk-2 /DEF=polo-like kinase 2
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      6grp_highest_nonorm > 91      6grp_total > 97      4grp_total > 32      6grp_highest_sub > Da      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 29.8
(-3)
A 		81.6
(-0.7)
P 		146.9
(2.1)
P 		167.3
(3)
P 		138
B 11.2
(-3)
A 		94.8
(1.5)
P 		99.8
(1.8)
P 		122.9
(3)
P 		112
C 23.4
(-3)
A 		85.3
(0.1)
P 		144.4
(3)
P 		83.7
(-0)
P 		121
avr 21.5
(-3) 		87.2
(0.6) 		130.4
(3) 		124.6
(2.7) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 469
1 2 3 4 5 6 range
A 62.3
(-2.8)
P 		47
(-3)
P 		76.3
(-2.5)
P 		103.1
(-2.1)
P 		332
(1.6)
P 		419.5
(3)
P 		373
B 49.6
(-3)
P 		52
(-3)
P 		59.2
(-2.9)
P 		111.6
(-2.2)
P 		284.3
(0.1)
P 		508.2
(3)
P 		459
C 63.4
(-2.6)
P 		38.9
(-3)
P 		51.4
(-2.8)
P 		108.6
(-1.9)
P 		326.7
(1.4)
P 		434.9
(3)
P 		396
avr 58.4
(-2.8) 		46
(-3) 		62.3
(-2.8) 		107.8
(-2.1) 		314.3
(0.9) 		454.2
(3) 		408
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 10.9
(-3)
A 		17.7
(-1.5)
P 		38.4
(3)
P 		24.5
(-0)
P 		28
B 3.6
(-3)
A 		23.4
(2.3)
P 		23.1
(2.2)
P 		26.2
(3)
P 		23
C 7.7
(-3)
A 		24.5
(-0.6)
P 		50.3
(3)
P 		14.1
(-2.1)
P 		43
avr 7.4
(-3) 		21.9
(-0.1) 		37.3
(3) 		21.6
(-0.2) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 11.5
(-3)
P 		16.7
(-2.7)
P 		16.6
(-2.7)
P 		26.1
(-2)
P 		100.2
(2.9)
P 		102.1
(3)
P 		91
B 17.3
(-2.9)
P 		17.3
(-2.9)
P 		14.4
(-3)
P 		29.6
(-2.5)
P 		115.5
(0.5)
P 		189.5
(3)
P 		175
C 23.5
(-2.5)
P 		11.9
(-3)
P 		13.1
(-2.9)
P 		21.8
(-2.6)
P 		105.7
(1.2)
P 		145.6
(3)
P 		134
avr 17.4
(-2.9) 		15.3
(-3) 		14.7
(-3) 		25.8
(-2.5) 		107.1
(1.2) 		145.7
(3) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173471_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22520
Sequence Type Exemplar sequence
Representative Public ID 6760364 NCBI
Target Description g6760364 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=Y71F9B.7 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=Y71F9B.7 /WP=CE22877 /CHR=1 /FEA=Genomic Cluster /GEN=plk-2 /DEF=polo-like kinase 2
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058635(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058635 NCBI Caenorhabditis elegans POLO Kinase family member (plk-2) (plk-2) mRNA, complete cds. 11/11 A 
Y71F9B.7 ENSEMBL cdna:known chromosome:CEL140:I:2755625:2758205:-1 gene:Y71F9B.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2755653-2758206(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol plk-2
Ensembl Y71F9B.7
Entrez Gene 171838 Entrez gene
SwissProt Q9N2L7 EMBL-EBI
WormBase CE22877 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36014 Polo kinase protein 2 [Caenorhabditis elegans] ref|NP_491036.1| POLO Kinase family member (plk-2) [Caenorhabditis elegans] gb|AAF28314.1| polo-like kinase 2 [Caenorhabditis elegans] sp|Q9N2L7|PLK2_CAEEL Serine/threonine-protein kinase plk-2 (Polo-like kinase-2) 0.0
blast CAE60501 Hypothetical protein CBG04120 [Caenorhabditis briggsae] 0.0
blast CAE64506 Hypothetical protein CBG09233 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-59
hanks 1.9.5 AGC group; AGC Other; DdK2 5.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR000959 EMBL-EBI POLO box duplicated region 1.2E-26
Pfam IPR000959 EMBL-EBI POLO box duplicated region 1.9E-27
Pfam IPR000719 EMBL-EBI Protein kinase 6.8E-84
Sequence
Target Sequence 
>C. ELEGANS:173471_AT
tcctcgcgtcgctaagaaaatcgatctgcaacaaattgagctcatttccccttaccggtt
ttgatatttctctgatcaatacacttttatgtccgtgtttgtaatcaattttatccaagc
aaattgccttccttttgtattgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCGCGTCGCTAAGAAAATCGAT 69 593 1898 Antisense
AAGAAAATCGATCTGCAACAAATTG 230 151 1911 Antisense
AACAAATTGAGCTCATTTCCCCTTA 608 135 1927 Antisense
CCTTACCGGTTTTGATATTTCTCTG 256 269 1947 Antisense
TACCGGTTTTGATATTTCTCTGATC 290 649 1950 Antisense
GATATTTCTCTGATCAATACACTTT 317 427 1960 Antisense
CTGATCAATACACTTTTATGTCCGT 466 239 1969 Antisense
CACTTTTATGTCCGTGTTTGTAATC 469 197 1979 Antisense
GTTTGTAATCAATTTTATCCAAGCA 12 453 1994 Antisense
TATCCAAGCAAATTGCCTTCCTTTT 647 657 2009 Antisense
CAAATTGCCTTCCTTTTGTATTGCC 55 195 2017 Antisense
Sequence Source GenBank