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[173455_s_at]

Description g476337 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=C26D10.2 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=C26D10.2 /WP=CE03025 /CHR=2 /FEA=Genomic Cluster /GEN=hel-1 /DEF=translation initiation factor eIF-4A
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 37      6grp_total > 10      4grp_total > 58      6grp_highest_sub > 02      6grp_cgc > 55      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4690
1 2 3 4 range
A 5608.3
(-3)
P 		8266.5
(2.3)
P 		8615.8
(3)
P 		5614.6
(-3)
P 		3008
B 7189.2
(-0.4)
P 		9494.9
(3)
P 		8700.4
(1.8)
P 		5384.4
(-3)
P 		4111
C 6601.6
(0.3)
P 		8046.7
(3)
P 		7850.2
(2.6)
P 		4804.7
(-3)
P 		3242
avr 6466.4
(-0.8) 		8602.7
(3) 		8388.8
(2.6) 		5267.9
(-3) 		3335

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2860
1 2 3 4 5 6 range
A 5079.7
(-1.1)
P 		6017
(3)
P 		5500.5
(0.7)
P 		5064.5
(-1.2)
P 		4642.7
(-3)
P 		5240.8
(-0.4)
P 		1374
B 4287.3
(-3)
P 		6531.9
(3)
P 		5605.4
(0.5)
P 		5454.6
(0.1)
P 		4380.5
(-2.8)
P 		4973.2
(-1.2)
P 		2245
C 4582
(-1.2)
P 		6606.8
(3)
P 		6085.2
(1.9)
P 		5540.8
(0.8)
P 		3746.9
(-3)
P 		5431.5
(0.5)
P 		2860
avr 4649.7
(-1.9) 		6385.2
(3) 		5730.4
(1.2) 		5353.3
(0.1) 		4256.7
(-3) 		5215.2
(-0.3) 		2129
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1926
1 2 3 4 range
A 2042
(2.1)
P 		1794.4
(1.1)
P 		2252.3
(3)
P 		821.9
(-3)
P 		1430
B 2292.6
(2.7)
P 		2345
(3)
P 		2016.1
(1.4)
P 		1147.4
(-3)
P 		1198
C 2185.3
(1.3)
P 		2313.5
(1.7)
P 		2736
(3)
P 		810.1
(-3)
P 		1926
avr 2173.3
(2.3) 		2151
(2.2) 		2334.8
(3) 		926.5
(-3) 		1408

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1238
1 2 3 4 5 6 range
A 936.4
(-3)
P 		2139.9
(3)
P 		1197.9
(-1.7)
P 		1284.5
(-1.3)
P 		1401
(-0.7)
P 		1276
(-1.3)
P 		1204
B 1491.8
(-2)
P 		2174.2
(3)
P 		1361.2
(-3)
P 		1444.7
(-2.4)
P 		1779.7
(0.1)
P 		1854.5
(0.6)
P 		813
C 1698.4
(0.9)
P 		2015.9
(3)
P 		1548.8
(-0.1)
P 		1114.2
(-3)
P 		1212.4
(-2.3)
P 		1818.2
(1.7)
P 		902
avr 1375.5
(-2.3) 		2110
(3) 		1369.3
(-2.4) 		1281.1
(-3) 		1464.4
(-1.7) 		1649.6
(-0.3) 		829
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173455_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23323
Sequence Type Exemplar sequence
Representative Public ID 476337 NCBI
Target Description g476337 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=C26D10.2 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=C26D10.2 /WP=CE03025 /CHR=2 /FEA=Genomic Cluster /GEN=hel-1 /DEF=translation initiation factor eIF-4A
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026814 NCBI Caenorhabditis elegans HELicase family member (hel-1) (hel-1) mRNA, complete cds. 11/11 None
SNAP00000007135 ENSEMBL cdna:SNAP chromosome:CEL140:II:8327018:8328792:-1 11/11 None
GENEFINDER00000007142 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8327018:8328792:-1 11/11 None
C26D10.2a ENSEMBL cdna:known chromosome:CEL140:II:8326775:8328792:-1 gene:C26D10.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026815 2/11 Cross Hyb Matching Probes None
C26D10.2b.1 2/11 Cross Hyb Matching Probes None
C26D10.2b.2 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174333 2 1 NM_001026814 173455_s_at (11), 174125_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8327015-8328790(-) 100.0 100.0
Public Domain and Genome References
Ensembl C26D10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91120 Hypothetical protein C26D10.2a [Caenorhabditis elegans] ref|NP_001021985.1| HELicase family member (hel-1) [Caenorhabditis elegans] sp|Q18212|UAP56_CAEEL Spliceosome RNA helicase BAT1 homolog (DEAD-box protein UAP56) 0.0
blast CAE57692 Hypothetical protein CBG00694 [Caenorhabditis briggsae] 0.0
blast AAB65852 putative RNA helicase 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.5E-28
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 3.5E-64
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.5E-28
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.3E-67
Sequence
Target Sequence 
>C. ELEGANS:173455_S_AT
aagcaagtgatgatgttctccgccacattgccaaaggagcttcgcactgtttgcaagcga
ttcatgcaagagccaatggaagtctacgtcgacgacgaagccaagctcactctgcacgga
cttcagcaacattacgtcaaactgaaggaagccgagaagaaccgtcgtttgctgaatctt
ctcgatgcactcgagttcaaccaagtcgtcattttcgtcaaggccgtaaaacgttgcgaa
gctctccaccagctcctcacagaacaaaatttcccatcaattgccattcatcgtcaaatg
gctcaagaagagcgactttcaagataccaagcattcaaagacttccaaaagagaatcttg
gtggccaccgatttgtttggacgtggaatggatatcgaaagagtcaacatcgtgttcaac
tacgacatgccagaagattccgactcgtatcttcacagagttgcacgtgctggtcgtttt
ggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCAAGTGATGATGTTCTCCGCCA 537 151 665 Antisense
AGGAGCTTCGCACTGTTTGCAAGCG 271 55 699 Antisense
ACTCTGCACGGACTTCAGCAACATT 654 99 773 Antisense
GTCGTTTGCTGAATCTTCTCGATGC 566 473 828 Antisense
GCACTCGAGTTCAACCAAGTCGTCA 48 317 851 Antisense
AAGTCGTCATTTTCGTCAAGGCCGT 629 157 867 Antisense
GTAAAACGTTGCGAAGCTCTCCACC 528 461 890 Antisense
CCCATCAATTGCCATTCATCGTCAA 605 257 937 Antisense
GGTGGCCACCGATTTGTTTGGACGT 355 497 1024 Antisense
AAGATTCCGACTCGTATCTTCACAG 84 157 1098 Antisense
GTTGCACGTGCTGGTCGTTTTGGAA 375 439 1124 Antisense
Sequence Source GenBank