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[173443_s_at]

Description g7407666 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F57B10.12 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F57B10.12 /WP=CE11320 /CHR=1 /FEA=Genomic Cluster /GEN=mei-2 /DEF=MEI-2
Cluster 6grp_total > 97      4grp_total > 09      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 65.8
(-3)
A 		90.5
(-1.5)
A 		87.2
(-1.7)
P 		165.2
(3)
P 		99
B 78
(-3)
P 		81.7
(-2.7)
A 		105.3
(-0.9)
P 		157
(3)
P 		79
C 98.4
(-3)
A 		123.1
(-1.9)
M 		121.2
(-2)
P 		236.2
(3)
P 		138
avr 80.7
(-3) 		98.4
(-2) 		104.6
(-1.6) 		186.1
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1551
1 2 3 4 5 6 range
A 86.7
(-3)
A 		85.5
(-3)
M 		99.9
(-2.9)
P 		187.3
(-2.4)
P 		936.9
(1.7)
P 		1182.5
(3)
P 		1097
B 64.7
(-3)
A 		73
(-3)
A 		144.9
(-2.7)
P 		259.3
(-2.2)
P 		947.9
(0.8)
P 		1454.9
(3)
P 		1390
C 70
(-3)
A 		71
(-3)
A 		128.9
(-2.8)
P 		254.4
(-2.3)
P 		820.9
(-0.1)
P 		1615.6
(3)
P 		1546
avr 73.8
(-3) 		76.5
(-3) 		124.6
(-2.8) 		233.7
(-2.3) 		901.9
(0.7) 		1417.7
(3) 		1344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 24
(2.7)
A 		19.6
(-3)
A 		22.8
(1.2)
P 		24.2
(3)
P 		5
B 24.9
(-0.9)
P 		20.2
(-3)
A 		24.4
(-1.1)
P 		33.4
(3)
P 		13
C 32.6
(-3)
A 		35.4
(-1.3)
M 		42.2
(3)
P 		39.8
(1.5)
P 		10
avr 27.2
(-1.3) 		25.1
(-3) 		29.8
(0.8) 		32.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 527
1 2 3 4 5 6 range
A 16
(-3)
A 		30.4
(-2.7)
M 		21.8
(-2.9)
P 		47.5
(-2.3)
P 		282.7
(2.9)
P 		287.9
(3)
P 		272
B 22.5
(-3)
A 		24.3
(-3)
A 		35.2
(-2.9)
P 		68.7
(-2.5)
P 		385.1
(1.2)
P 		542.5
(3)
P 		520
C 26
(-3)
A 		21.7
(-3)
A 		32.8
(-2.9)
P 		51.2
(-2.7)
P 		265.6
(-0.2)
P 		540.8
(3)
P 		519
avr 21.5
(-3) 		25.5
(-2.9) 		29.9
(-2.9) 		55.8
(-2.5) 		311.1
(1) 		457.1
(3) 		436
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173443_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22887
Sequence Type Exemplar sequence
Representative Public ID 7407666 NCBI
Target Description g7407666 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F57B10.12 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F57B10.12 /WP=CE11320 /CHR=1 /FEA=Genomic Cluster /GEN=mei-2 /DEF=MEI-2
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059493(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059493 NCBI Caenorhabditis elegans defective MEIosis family member (mei-2) (mei-2) mRNA, complete cds. 11/11 None
SNAP00000011833 ENSEMBL cdna:SNAP chromosome:CEL140:I:6573002:6573984:-1 11/11 None
GENEFINDER00000011837 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6573002:6573984:-1 11/11 None
F57B10.12 ENSEMBL cdna:known chromosome:CEL140:I:6572883:6573986:-1 gene:F57B10.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6572885-6573914(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mei-2
Ensembl F57B10.12
Entrez Gene 172374 Entrez gene
SwissProt O44740 EMBL-EBI
WormBase CE11320 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7019 microtubule depolymerization inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB96729 Defective meiosis protein 2 [Caenorhabditis elegans] ref|NP_491894.1| defective MEIosis family member (mei-2) [Caenorhabditis elegans] gb|AAF62184.1| MEI-2 [Caenorhabditis elegans] sp|O44740|MEI2_CAEEL Meiotic spindle formation protein 1.0E-136
blast CAB16313 Hypothetical protein F47G4.4 [Caenorhabditis elegans] ref|NP_493453.1| F47G4.4 [Caenorhabditis elegans] 2.0E-35
blast CAE67237 Hypothetical protein CBG12676 [Caenorhabditis briggsae] 8.0E-35
Sequence
Target Sequence 
>C. ELEGANS:173443_S_AT
agcagttctttgccaaaatcctccccagaatcatccgtgtcagtcatgtcatcaaacgcg
tcgttgtggtccgcatgcaccgaggaagtgaataaaatcggtgtttgtgcgaagcgagag
agcagagatcttcgtgtttataagatgaagagtttcacaagtaacatggaacagatattg
tcaaacgacaaccaactcgctcccacagtcattagaattctgaatagtagaaactcttgg
tgtctcaactcgtgccatgcttgtttaacatttatcatggaaaatatcacgagcgacaat
tatgggaagcgatcggcttgcttgaaggctcttgcatcaattaccaacagtttactggac
acaatcatcggatttgcatccaccaaaactcggcgaattggagtcgatgtggtagctgaa
gagcgagctgcaaaagcaacagagtgcattcacaattttcgcaaaatagttaaaaatcgg
gacaaaatctacaaacagattgaccaggagacaatttacaaactcgatgccattctcgag
cgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGTTCTTTGCCAAAATCCTCCC 411 77 287 Antisense
GAATCATCCGTGTCAGTCATGTCAT 72 329 314 Antisense
CAGTCATGTCATCAAACGCGTCGTT 664 207 327 Antisense
GGTCCGCATGCACCGAGGAAGTGAA 437 499 354 Antisense
TTGTCAAACGACAACCAACTCGCTC 623 707 464 Antisense
TCGCTCCCACAGTCATTAGAATTCT 80 599 483 Antisense
AGAAACTCTTGGTGTCTCAACTCGT 311 71 515 Antisense
TCAACTCGTGCCATGCTTGTTTAAC 251 631 531 Antisense
GGCTTGCTTGAAGGCTCTTGCATCA 576 537 601 Antisense
TGCATCCACCAAAACTCGGCGAATT 105 579 661 Antisense
AACTCGATGCCATTCTCGAGCGATT 617 141 807 Antisense
Sequence Source GenBank