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[173441_s_at]

Description g6690645 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F54C8.3 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F54C8.3 /WP=CE26428 /CHR=3 /FEA=Genomic Cluster /GEN=emb-30 /DEF=emb-30 protein
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 82      6grp_total > 48      4grp_total > 55      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 457
1 2 3 4 range
A 493.3
(3)
P 		150.4
(-3)
P 		281
(-0.7)
P 		237
(-1.5)
P 		343
B 492.8
(3)
P 		347.9
(-0)
P 		328.7
(-0.4)
P 		203.3
(-3)
P 		290
C 607.1
(3)
P 		303.4
(-3)
P 		352.5
(-2)
P 		393.2
(-1.2)
P 		304
avr 531.1
(3) 		267.2
(-3) 		320.7
(-1.8) 		277.8
(-2.8) 		264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 360.6
(-0.8)
P 		404.5
(-0.1)
P 		264.7
(-2.3)
P 		220.2
(-3)
P 		371.6
(-0.6)
P 		597.1
(3)
P 		377
B 494.2
(1.7)
P 		386.9
(0.1)
P 		174.1
(-3)
P 		472.3
(1.4)
P 		403.4
(0.4)
P 		580.1
(3)
P 		406
C 459.9
(2.2)
P 		508
(3)
P 		361.2
(0.5)
P 		156.5
(-3)
P 		302
(-0.5)
P 		241.1
(-1.6)
P 		352
avr 438.2
(2) 		433.1
(1.8) 		266.7
(-3) 		283
(-2.5) 		359
(-0.3) 		472.8
(3) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 179.6
(3)
P 		32.6
(-3)
P 		73.4
(-1.3)
P 		34.7
(-2.9)
P 		147
B 157.2
(3)
P 		85.9
(-0.8)
P 		76.2
(-1.3)
P 		43.3
(-3)
P 		114
C 201
(3)
P 		87.2
(-2.1)
P 		122.9
(-0.5)
P 		66.3
(-3)
P 		135
avr 179.3
(3) 		68.6
(-2.1) 		90.8
(-1) 		48.1
(-3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 66.5
(-2.3)
P 		143.9
(2.9)
P 		57.6
(-2.9)
P 		55.8
(-3)
P 		112.1
(0.8)
P 		145.4
(3)
P 		90
B 172
(1.5)
P 		128.8
(-0)
P 		42.3
(-3)
P 		125.1
(-0.1)
P 		163.9
(1.2)
P 		216.3
(3)
P 		174
C 170.5
(3)
P 		155
(2.3)
P 		91.9
(-0.4)
P 		31.5
(-3)
P 		97.7
(-0.1)
P 		80.7
(-0.9)
P 		139
avr 136.3
(2.2) 		142.6
(2.6) 		63.9
(-3) 		70.8
(-2.5) 		124.6
(1.4) 		147.5
(3) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173441_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24072
Sequence Type Exemplar sequence
Representative Public ID 6690645 NCBI
Target Description g6690645 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=F54C8.3 /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=F54C8.3 /WP=CE26428 /CHR=3 /FEA=Genomic Cluster /GEN=emb-30 /DEF=emb-30 protein
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066673(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066673 NCBI Caenorhabditis elegans abnormal EMBroygenesis family member (emb-30) (emb-30) mRNA, complete cds. 11/11 A 
F54C8.3 ENSEMBL cdna:known chromosome:CEL140:III:9434979:9441356:-1 gene:F54C8.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9435227-9441351(-) 99.36 99.82
Public Domain and Genome References
Gene Title
Gene Symbol emb-30
Ensembl F54C8.3
Entrez Gene 176322 Entrez gene
SwissProt P34441 EMBL-EBI
WormBase CE32676 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80159 Hypothetical protein F54C8.3 [Caenorhabditis elegans] ref|NP_499074.2| abnormal EMBroygenesis family member (emb-30) [Caenorhabditis elegans] gb|AAF24233.1| emb-30 protein [Caenorhabditis elegans] sp|P34441|EMB30_CAEEL Abnormal embryogenesis protein 0.0
blast G88554 protein F54C8.3 [imported] - Caenorhabditis elegans 0.0
blast CAE65218 Hypothetical protein CBG10093 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:173441_S_AT
gatgacatttagtttccaatcgatggataaattattgcatttctatgtttatatttcgta
ttactttgaaattatttctcctttccaatctattgcatttcttttcttggctttccctct
tacaatctcattttgctttttgcgggcacctataaacgtgtttttagtcaaaactcgttt
cttctagttttagtttgatgcaaatgtaaggcttgagtttcaaagttttcaagttttcaa
taattcatctttattttttataatcaggaagctgatatcttacgagggcgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGACATTTAGTTTCCAATCGATG 64 413 3089 Antisense
GCATTTCTATGTTTATATTTCGTAT 221 309 3125 Antisense
AAATTATTTCTCCTTTCCAATCTAT 385 115 3157 Antisense
TCTCCTTTCCAATCTATTGCATTTC 188 611 3165 Antisense
TATTGCATTTCTTTTCTTGGCTTTC 398 665 3179 Antisense
TTTCCCTCTTACAATCTCATTTTGC 176 671 3200 Antisense
TCATTTTGCTTTTTGCGGGCACCTA 279 615 3216 Antisense
TGCGGGCACCTATAAACGTGTTTTT 653 573 3229 Antisense
TAGTCAAAACTCGTTTCTTCTAGTT 80 655 3253 Antisense
GTTTCTTCTAGTTTTAGTTTGATGC 56 451 3265 Antisense
GAAGCTGATATCTTACGAGGGCGTA 269 343 3356 Antisense
Sequence Source GenBank