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[173426_at] p21 ras-related rho (RhoA)

Description g558500-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g558500 /5_PRIME_EXT_DB=GenBank Identifier /CHR=4 /FEA=Genomic Cluster /GEN=RhoA /DEF=guanine nucleotide regulatory protein
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 38      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 80      6grp_total > 27      4grp_total > 53      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 569.5
(3)
P 		484.7
(0.6)
P 		448.6
(-0.4)
P 		356.8
(-3)
P 		213
B 597
(3)
P 		484.6
(0.5)
P 		456.1
(-0.1)
P 		326.6
(-3)
P 		270
C 603.5
(3)
P 		514.4
(1.2)
P 		502.5
(0.9)
P 		308.3
(-3)
P 		295
avr 590
(3) 		494.6
(0.8) 		469.1
(0.2) 		330.6
(-3) 		259

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 371.9
(3)
P 		195.5
(-1.2)
P 		175
(-1.6)
P 		117.5
(-3)
P 		174.5
(-1.7)
P 		263.6
(0.4)
P 		254
B 293.3
(3)
P 		219.8
(0.5)
P 		195.3
(-0.3)
P 		158.4
(-1.6)
P 		116.9
(-3)
P 		242.6
(1.3)
P 		176
C 407.4
(3)
P 		186.9
(-1.9)
P 		166.5
(-2.3)
P 		140.1
(-2.9)
P 		136.6
(-3)
P 		265.8
(-0.1)
P 		271
avr 357.5
(3) 		200.7
(-1.3) 		178.9
(-1.9) 		138.7
(-3) 		142.7
(-2.9) 		257.3
(0.3) 		219
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 207.3
(3)
P 		105.2
(-0.9)
P 		117.3
(-0.5)
P 		52.2
(-3)
P 		155
B 190.4
(3)
P 		119.7
(-0.5)
P 		105.7
(-1.2)
P 		69.6
(-3)
P 		121
C 199.8
(3)
P 		147.9
(0.9)
P 		175.1
(2)
P 		52
(-3)
P 		148
avr 199.2
(3) 		124.3
(-0.2) 		132.7
(0.2) 		57.9
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 68.5
(2.8)
P 		69.5
(3)
P 		38.1
(-1.7)
P 		29.8
(-3)
P 		52.6
(0.4)
P 		64.2
(2.2)
P 		40
B 102.1
(3)
P 		73.2
(0.1)
P 		47.4
(-2.5)
P 		41.9
(-3)
P 		47.5
(-2.4)
P 		90.5
(1.8)
P 		60
C 151
(3)
P 		57
(-1.6)
P 		42.4
(-2.3)
P 		28.2
(-3)
P 		44.2
(-2.2)
P 		89
(-0)
P 		123
avr 107.2
(3) 		66.6
(-0.3) 		42.6
(-2.2) 		33.3
(-3) 		48.1
(-1.8) 		81.2
(0.9) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173426_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24533
Sequence Type Exemplar sequence
Representative Public ID 558500 NCBI
Target Description g558500-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g558500 /5_PRIME_EXT_DB=GenBank Identifier /CHR=4 /FEA=Genomic Cluster /GEN=RhoA /DEF=guanine nucleotide regulatory protein
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070558 NCBI Caenorhabditis elegans RHO (small G protein) family member (rho-1) (rho-1) mRNA, complete cds. 11/11 A 
Y51H4A.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:16482496:16488981:-1 gene:Y51H4A.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Y51H4A.3.2 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16483229-16488983(-) 99.56 99.56
Public Domain and Genome References
Gene Title p21 ras-related rho (RhoA)
Gene Symbol rho-1
Ensembl Y51H4A.3
Entrez Gene 178458 Entrez gene
SwissProt Q22038 EMBL-EBI
WormBase CE25369 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604207_AT ras homolog gene family, member A cfa
CANINE_2:CFA.20231.1.A1_S_AT ras homolog gene family, member A cfa
CANINE_2:CFA.20231.1.A2_AT ras homolog gene family, member A cfa
DROSOPHILA_2:1626808_S_AT glass multimer reporter construct of Hariharan dm
DROSOPHILA_2:1625488_S_AT glass multimer reporter construct of Hariharan dm
DROSGENOME1:152814_AT glass multimer reporter construct of Hariharan dm
CHICKEN:GGA.4963.1.S1_AT ras homolog gene family, member A gga
CHICKEN:GGA.4963.2.S1_A_AT ras homolog gene family, member A gga
CHICKEN:GGAAFFX.12357.1.S1_S_AT ras homolog gene family, member A gga
PLASMODIUM_ANOPHELES:200059_S_AT ras homolog gene family, member A hs
HG-U133B:200059_S_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:200059_S_AT ras homolog gene family, member A hs
HG-FOCUS:200059_S_AT ras homolog gene family, member A hs
HG-U133A_2:200059_S_AT ras homolog gene family, member A hs
HG-U133A:200059_S_AT ras homolog gene family, member A hs
U133_X3P:200059_S_AT ras homolog gene family, member A hs
HG-U95AV2:1394_AT ras homolog gene family, member A hs
HC-G110:1394_AT ras homolog gene family, member A hs
HG-U95AV2:37309_AT ras homolog gene family, member A hs
HUGENEFL:L25080_AT ras homolog gene family, member A hs
U133_X3P:HS2.77273.2.S1_3P_S_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:1555814_A_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:240337_AT Ras homolog gene family, member A hs
HG-U133B:240337_AT Ras homolog gene family, member A hs
HG-U133_PLUS_2:241097_AT Ras homolog gene family, member A hs
HG-U133B:241097_AT Ras homolog gene family, member A hs
HG-U95E:69748_R_AT Ras homolog gene family, member A hs
U133_X3P:HS.201173.0.A1_3P_AT Ras homolog gene family, member A hs
MU11KSUBA:AA161790_S_AT ras homolog gene family, member A mm
MU11KSUBB:MSA.3406.0_S_AT ras homolog gene family, member A mm
MG-U74AV2:101112_G_AT ras homolog gene family, member A mm
MG-U74AV2:101113_AT ras homolog gene family, member A mm
MOE430A:1437628_S_AT ras homolog gene family, member A mm
MOUSE430A_2:1437628_S_AT ras homolog gene family, member A mm
MOUSE430_2:1437628_S_AT ras homolog gene family, member A mm
MG-U74AV2:101111_AT ras homolog gene family, member A mm
MG-U74CV2:134677_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBA:TC14290_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBA:TC24964_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBC:TC39157_F_AT Ras homolog gene family, member A (Rhoa), mRNA mm
RG-U34A:D84477_AT ras homolog gene family, member A rn
RG-U34C:RC_AI179356_AT ras homolog gene family, member A rn
RG-U34C:RC_AA819664_AT ras homolog gene family, member A rn
RAE230A:1399027_AT ras homolog gene family, member A rn
RAT230_2:1399027_AT ras homolog gene family, member A rn
RAE230A:1370130_AT ras homolog gene family, member A rn
RAT230_2:1370130_AT ras homolog gene family, member A rn
RG-U34B:RC_AA964181_AT Ras homolog gene family, member A rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63379 Hypothetical protein Y51H4A.3 [Caenorhabditis elegans] ref|NP_502959.1| RHO (small G protein) family member (rho-1) [Caenorhabditis elegans] sp|Q22038|RHOA_CAEEL RAS-like GTP-binding protein RhoA gb|AAC37216.1| guanine nucleotide regulatory protein emb|CAE68045.1| Hypothetical protein CBG13664 [Caenorhabditis briggsae] 1.0E-97
blast AAY56741 guanine nucleotide regulatory protein [Caenorhabditis remanei] 3.0E-96
blast EAA44533 ENSANGP00000024640 [Anopheles gambiae str. PEST] gb|EAA09459.2| ENSANGP00000015684 [Anopheles gambiae str. PEST] ref|XP_314052.2| ENSANGP00000015684 [Anopheles gambiae str. PEST] ref|XP_314051.1| ENSANGP00000024640 [Anopheles gambiae str. PEST] 5.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.6E-94
Sequence
Target Sequence 
>C. ELEGANS:173426_AT
atttgaaattcgcgcttctctattccaattttttgaggtggtggccgtggccttgaaact
tgaaaaagttaggccaccgctctctctctctttctctctgaaattccaaatcacacatct
cctctcccgcccacacacacacacacattttatagcttaatccccgaattccccgaatta
aaaaaaaaatttccgtttttttttttgattttcacaatgtcaaactgtgttattcctccg
ttacccctccgacgcgcttacacacttttttctttaaaaaataattttttctctgccaaa
atcactaatttttcatttttattgaaaaaacctccatcgccccatcgctactttcctatc
ttagcttcaatgtaacaggtttctatttttttttcgtcgaattttttttcctagcgagct
ttagccaaggatgccgtttttcgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTGAAATTCGCGCTTCTCTATTC 688 17 750 Antisense
GCTTCTCTATTCCAATTTTTTGAGG 311 305 763 Antisense
GGTGGCCGTGGCCTTGAAACTTGAA 233 499 789 Antisense
CTCTCTTTCTCTCTGAAATTCCAAA 194 231 835 Antisense
ACACATTTTATAGCTTAATCCCCGA 16 107 892 Antisense
GTCAAACTGTGTTATTCCTCCGTTA 472 463 968 Antisense
TTTTTTCTCTGCCAAAATCACTAAT 611 675 1034 Antisense
CTACTTTCCTATCTTAGCTTCAATG 526 225 1097 Antisense
GTCGAATTTTTTTTCCTAGCGAGCT 503 469 1145 Antisense
AGCGAGCTTTAGCCAAGGATGCCGT 648 81 1162 Antisense
TAGCCAAGGATGCCGTTTTTCGACG 600 651 1171 Antisense
Sequence Source GenBank