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[173424_at]

Description g2944397-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g2944397 /5_PRIME_EXT_DB=GenBank Identifier /CHR=1 /FEA=Genomic Cluster /GEN=unc-73 /DEF=guanine nucleotide exchange factor UNC-73B
Cluster 6grp_total > 18      4grp_total > 06      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 167.5
(3)
A 		74.8
(-3)
A 		133
(0.8)
A 		140.3
(1.2)
A 		93
B 138.4
(0.2)
A 		102
(-1.8)
A 		79.5
(-3)
A 		189.4
(3)
A 		110
C 143
(-0.9)
A 		87
(-3)
A 		91.9
(-2.8)
A 		244.4
(3)
A 		157
avr 149.6
(0.6) 		87.9
(-3) 		101.5
(-2.2) 		191.4
(3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 308.7
(3)
A 		98.5
(-3)
A 		112.9
(-2.6)
A 		110
(-2.7)
A 		217.4
(0.4)
A 		176.3
(-0.8)
A 		210
B 214.7
(3)
P 		190.9
(1.8)
A 		117.2
(-1.9)
A 		114.9
(-2)
A 		95.9
(-3)
A 		123.1
(-1.6)
A 		119
C 340.5
(3)
P 		170.1
(-1.5)
A 		119.2
(-2.8)
A 		153.9
(-1.9)
A 		113.5
(-3)
A 		127.5
(-2.6)
A 		227
avr 288
(3) 		153.2
(-1.7) 		116.4
(-3) 		126.3
(-2.7) 		142.3
(-2.1) 		142.3
(-2.1) 		172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 61
(3)
A 		16.2
(-3)
A 		34.8
(-0.5)
A 		20.5
(-2.4)
A 		45
B 44.1
(3)
A 		25.2
(-1.4)
A 		18.4
(-3)
A 		40.4
(2.1)
A 		26
C 47.3
(3)
A 		25
(-3)
A 		32
(-1.1)
A 		41.2
(1.4)
A 		22
avr 50.8
(3) 		22.1
(-3) 		28.4
(-1.7) 		34
(-0.5) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 56.9
(1.7)
A 		35
(-1.5)
A 		24.6
(-3)
A 		27.9
(-2.5)
A 		65.6
(3)
A 		42.9
(-0.3)
A 		41
B 74.7
(3)
P 		63.5
(1.5)
A 		28.5
(-3)
A 		30.4
(-2.8)
A 		38.9
(-1.6)
A 		45.9
(-0.7)
A 		46
C 126.2
(3)
P 		51.9
(-1.6)
A 		30.3
(-3)
A 		31
(-3)
A 		36.7
(-2.6)
A 		42.7
(-2.2)
A 		96
avr 85.9
(3) 		50.1
(-0.7) 		27.8
(-3) 		29.8
(-2.8) 		47.1
(-1) 		43.8
(-1.3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22521
Sequence Type Exemplar sequence
Representative Public ID 2944397 NCBI
Target Description g2944397-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g2944397 /5_PRIME_EXT_DB=GenBank Identifier /CHR=1 /FEA=Genomic Cluster /GEN=unc-73 /DEF=guanine nucleotide exchange factor UNC-73B
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) AF048835(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
AF048835 NCBI Caenorhabditis elegans guanine nucleotide exchange factor UNC-73B (unc-73) mRNA, complete cds. 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4015174-4031741(-) 98.49 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71109 Uncoordinated protein 73, isoform b [Caenorhabditis elegans] ref|NP_001021497.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12932.1| guanine nucleotide exchange factor UNC-73B [Caenorhabditis elegans] 0.0
blast AAT68892 Uncoordinated protein 73, isoform f [Caenorhabditis elegans] ref|NP_001021501.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast AAC71108 Uncoordinated protein 73, isoform a [Caenorhabditis elegans] ref|NP_001021496.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12931.1| guanine nucleotide exchange factor UNC-73A [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.7E-9
Pfam IPR000219 EMBL-EBI DH domain 2.4E-18
Sequence
Target Sequence 
>C. ELEGANS:173424_AT
gtgcgtgtggttgttgtgtcagtgattcgtgcatgtgctacattcatcaccgtcatcatc
ttcttttactttttctactttttctactcattttcttttttctttgatttttcctgcttg
tttctcctcttttcttccaagttctcctccatcatattcccaacttgattaaccaagtgc
ggagcagctcccaaagcttaatgagcaagaagagggagaggtcctcctcgtccttttttc
tttcattttcttcttgattttttgttgtctccatgaattggatggatacgacgttggcga
cgaagatggaggaaaagcaaacaattgtattcctgcatctccatctccaactctcctctt
tcatttttttcctccattattagactgaaaagcacctcgggtttttttccttgaacccta
ctgatcagattttgatttgccatatcattgccaacctccttatttattttgcaacttgat
ctcattattttttgttttccctactttttgtttcaaatttatttccttatgatttgctgc
tgtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCGTGTGGTTGTTGTGTCAGTGA 556 477 5046 Antisense
GTGTCAGTGATTCGTGCATGTGCTA 540 485 5061 Antisense
TGTGCTACATTCATCACCGTCATCA 393 557 5079 Antisense
CGTCATCATCTTCTTTTACTTTTTC 174 247 5096 Antisense
TCCTCCATCATATTCCCAACTTGAT 488 593 5190 Antisense
TTAACCAAGTGCGGAGCAGCTCCCA 68 685 5214 Antisense
AGAAGAGGGAGAGGTCCTCCTCGTC 293 73 5253 Antisense
TTTTTTCCTTGAACCCTACTGATCA 621 675 5448 Antisense
GATTTGCCATATCATTGCCAACCTC 4 429 5479 Antisense
TGCCAACCTCCTTATTTATTTTGCA 280 587 5494 Antisense
ATTTCCTTATGATTTGCTGCTGTAC 521 17 5566 Antisense
Sequence Source GenBank