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[173413_at]

Description g289715-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g289715 /5_PRIME_EXT_DB=GenBank Identifier /CHR=4 /FEA=Genomic Cluster /DEF=raf proto-oncogene
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 57      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 87      6grp_total > 48      4grp_total > 04      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1940
1 2 3 4 range
A 1426
(-1.4)
P 		971.6
(-2.8)
P 		906
(-3)
P 		2826.2
(3)
P 		1920
B 1464.1
(-0.3)
P 		931
(-2.8)
P 		886.3
(-3)
P 		2158
(3)
P 		1272
C 1310.6
(-1.4)
P 		1021.6
(-2.6)
P 		937.1
(-3)
P 		2315.1
(3)
P 		1378
avr 1400.2
(-1.1) 		974.7
(-2.7) 		909.8
(-3) 		2433.1
(3) 		1523

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1080
1 2 3 4 5 6 range
A 1687
(2.4)
P 		1170.6
(-0.7)
P 		887.9
(-2.4)
P 		789.8
(-3)
P 		1146.2
(-0.8)
P 		1782.6
(3)
P 		993
B 1401.1
(1.9)
P 		1022.1
(-0.7)
P 		702.6
(-3)
P 		1166.4
(0.3)
P 		810.3
(-2.2)
P 		1553.2
(3)
P 		851
C 1147.1
(-0.2)
P 		1019.6
(-1.6)
P 		1123
(-0.4)
P 		1071.2
(-1)
P 		889.4
(-3)
P 		1436.3
(3)
P 		547
avr 1411.7
(1.4) 		1070.8
(-1.5) 		904.5
(-3) 		1009.1
(-2.1) 		948.6
(-2.6) 		1590.7
(3) 		686
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 314
1 2 3 4 range
A 519.2
(3)
P 		210.9
(-3)
P 		236.8
(-2.5)
P 		413.7
(0.9)
P 		308
B 466.9
(3)
P 		229.9
(-2.4)
P 		205.4
(-3)
P 		459.9
(2.8)
P 		262
C 433.8
(3)
P 		293.7
(-3)
P 		326.6
(-1.6)
P 		390.4
(1.1)
P 		140
avr 473.3
(3) 		244.8
(-3) 		256.3
(-2.7) 		421.3
(1.6) 		229

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 409
1 2 3 4 5 6 range
A 311
(-0.1)
P 		416.3
(2.6)
P 		193.4
(-3)
P 		200.3
(-2.8)
P 		345.9
(0.8)
P 		434
(3)
P 		241
B 487.5
(1.7)
P 		340.2
(-0.5)
P 		170.6
(-3)
P 		308.9
(-1)
P 		329.2
(-0.7)
P 		579.2
(3)
P 		409
C 425.2
(1.7)
P 		311.1
(-0.8)
P 		285.8
(-1.4)
P 		215.4
(-3)
P 		287.8
(-1.4)
P 		480.8
(3)
P 		265
avr 407.9
(1.1) 		355.9
(-0) 		216.6
(-3) 		241.5
(-2.5) 		321
(-0.8) 		498
(3) 		281
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173413_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24528
Sequence Type Exemplar sequence
Representative Public ID 289715 NCBI
Target Description g289715-REP_DB=GenBank Identifier /5_PRIME_EXT_ID=g289715 /5_PRIME_EXT_DB=GenBank Identifier /CHR=4 /FEA=Genomic Cluster /DEF=raf proto-oncogene
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171367(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171367 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-45) (lin-45) mRNA, complete cds. 11/11 A 
Y73B6A.5a ENSEMBL cdna:known chromosome:CEL140:IV:6742522:6747450:-1 gene:Y73B6A.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177436 2 1 NM_171367 173413_at (11), 191672_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6742528-6747449(-) 99.34 100.0
Public Domain and Genome References
Ensembl Y73B6A.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF36042 Abnormal cell lineage protein 45, isoform a [Caenorhabditis elegans] ref|NP_741430.3| abnormal cell LINeage family member (lin-45) [Caenorhabditis elegans] gb|AAR86712.1| LIN-45 isoform 2 [Caenorhabditis elegans] gb|AAR26307.1| LIN-45 [Caenorhabditis elegans] sp|Q07292|KRAF1_CAEEL Raf homolog serine/threonine-protein kinase (Abnormal cell lineage protein 45) 0.0
blast AAA28142 raf proto-oncogene prf||1913436A lraf protein 0.0
blast AAR86713 LIN-45 isoform 3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.2E-18
Pfam IPR000719 EMBL-EBI Protein kinase 3.2E-68
Pfam IPR003116 EMBL-EBI Raf-like Ras-binding 1.3E-35
Sequence
Target Sequence 
>C. ELEGANS:173413_AT
atatgtacctttattttctctcttttttctattcttcccagtgattttccctaccccatg
tgtacactaaccaaaaaacttccagtcgaagaataatctcgcccatcgttattttactaa
tcatttctcgtcacattgaatcctgttctattattcaccccctcatcacccatcccgata
ttattattattattttaaccccgtattcaccgtttcctccaaaacttaaaaatcccacca
aaatactttctttccagtctaagtccgcctgcaatcgttcccccatgtgaaattgtgata
attttcatatttttgtttcggctaaaccggtagaactttttcttttccaactataatgct
ttttattgaaaacgtttcttttgataagttttcaatgttcccaccccatttccgtttcca
aacatcccaaagattctctgtatgtatagaattatatagaatccaatgcctatccgtttt
gtttttgtcatttcgaacacattttgaaacttccatttattttaatttcaaaatgcagca
tccccattcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATGTACCTTTATTTTCTCTCTTT 124 21 2583 Antisense
TTTCTATTCTTCCCAGTGATTTTCC 72 673 2608 Antisense
CCTACCCCATGTGTACACTAACCAA 430 265 2632 Antisense
ATAATCTCGCCCATCGTTATTTTAC 7 23 2675 Antisense
TTCTCGTCACATTGAATCCTGTTCT 458 693 2707 Antisense
TCATCACCCATCCCGATATTATTAT 223 621 2745 Antisense
TACTTTCTTTCCAGTCTAAGTCCGC 556 643 2826 Antisense
GCAATCGTTCCCCCATGTGAAATTG 516 321 2853 Antisense
TGATAAGTTTTCAATGTTCCCACCC 413 563 2964 Antisense
ATGCCTATCCGTTTTGTTTTTGTCA 293 39 3048 Antisense
TTCAAAATGCAGCATCCCCATTCCT 131 685 3109 Antisense
Sequence Source GenBank