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[173395_s_at] PROLYL 4-HYDROXYLASE ALPHA SUBUNIT PRECURSOR (EC 1.14.11.2)

Description AV199601_rc /REP_DB=TREMBL Accession /GB=AV199601 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk548g10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      6grp_highest_nonorm > 63      6grp_total > 81      4grp_total > 50      6grp_highest_sub > 04      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 512
1 2 3 4 range
A 286
(-0.6)
P 		596
(3)
P 		595.8
(3)
P 		84.3
(-3)
A 		512
B 215.4
(-2.3)
P 		592.1
(3)
P 		332.3
(-0.7)
P 		165.2
(-3)
A 		427
C 270.5
(-0.7)
P 		537.1
(3)
P 		336.7
(0.2)
P 		101.8
(-3)
A 		435
avr 257.3
(-1.2) 		575.1
(3) 		421.6
(1) 		117.1
(-3) 		458

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 725
1 2 3 4 5 6 range
A 343.8
(-3)
P 		411.1
(-2.3)
P 		780.1
(1.7)
P 		737.2
(1.2)
P 		903.7
(3)
P 		469.8
(-1.6)
P 		560
B 473.1
(-1.7)
P 		320.1
(-3)
P 		667.4
(-0.1)
P 		1044.6
(3)
P 		625.3
(-0.5)
P 		571.3
(-0.9)
P 		725
C 505.3
(-1)
P 		386.5
(-2.8)
P 		376.2
(-2.9)
P 		783.2
(3)
P 		628.3
(0.7)
P 		371.1
(-3)
P 		412
avr 440.7
(-2.2) 		372.6
(-3) 		607.9
(-0.1) 		855
(3) 		719.1
(1.3) 		470.7
(-1.8) 		482
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 104.1
(0.8)
P 		129.4
(1.9)
P 		155.8
(3)
P 		12.3
(-3)
A 		144
B 68.7
(-1.2)
P 		146.2
(3)
P 		77
(-0.7)
P 		35.2
(-3)
A 		111
C 89.6
(0.2)
P 		154.4
(3)
P 		117.3
(1.4)
P 		17.2
(-3)
A 		137
avr 87.5
(0.2) 		143.3
(3) 		116.7
(1.7) 		21.6
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 63.4
(-3)
P 		146.2
(-0.6)
P 		169.9
(0.1)
P 		187
(0.5)
P 		272.7
(3)
P 		114.4
(-1.5)
P 		209
B 164.6
(-1)
P 		106.6
(-3)
P 		162.1
(-1)
P 		276.7
(3)
P 		254
(2.2)
P 		213.1
(0.8)
P 		170
C 187.3
(2.1)
P 		117.9
(-1.8)
P 		95.7
(-3)
P 		157.5
(0.4)
P 		203.3
(3)
P 		124.2
(-1.4)
P 		108
avr 138.4
(-2.3) 		123.6
(-3) 		142.6
(-2) 		207.1
(1.2) 		243.3
(3) 		150.6
(-1.6) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173395_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27622
Sequence Type Exemplar sequence
Representative Public ID AV199601 NCBI
Target Description AV199601_rc /REP_DB=TREMBL Accession /GB=AV199601 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk548g10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067063(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067063 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-18) (dpy-18) mRNA, complete cds. 11/11 A 
Y47D3B.10.1 ENSEMBL cdna:known chromosome:CEL140:III:11372436:11377856:-1 gene:Y47D3B.10 11/11 A 
Y47D3B.10.2 ENSEMBL cdna:known chromosome:CEL140:III:11372559:11377856:-1 gene:Y47D3B.10 11/11 A 
Y47D3B.10.4 ENSEMBL cdna:known chromosome:CEL140:III:11372758:11378330:-1 gene:Y47D3B.10 11/11 A 
Y47D3B.10.3 ENSEMBL cdna:known chromosome:CEL140:III:11372759:11377856:-1 gene:Y47D3B.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11372845-11373266(-) 79.8 79.8
Public Domain and Genome References
Gene Title PROLYL 4-HYDROXYLASE ALPHA SUBUNIT PRECURSOR (EC 1.14.11.2)
Gene Symbol dpy-18
Ensembl Y47D3B.10
Entrez Gene 176569 Entrez gene
SwissProt Q10576 EMBL-EBI
WormBase CE20261 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264592_AT oxidoreductase, 2OG-Fe(II) oxygenase family protein at
ATGENOME1:16288_AT oxidoreductase, 2OG-Fe(II) oxygenase family protein at
CANINE:1594163_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFA.13303.2.S1_A_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFA.18668.1.S1_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFA.18668.1.S1_S_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFAAFFX.2160.1.S1_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFAAFFX.2160.1.S1_S_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CANINE_2:CFAAFFX.2161.1.S1_AT similar to Prolyl 4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit) cfa
CHICKEN:GGA.5799.1.S1_S_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II gga
CHICKEN:GGAAFFX.11832.1.S1_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II gga
HG-U133_PLUS_2:202733_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-FOCUS:202733_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U133A:202733_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U133A_2:202733_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HU35KSUBA:W49521_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HU35KSUBB:RC_F09788_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
U133_X3P:G4758867_3P_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U95AV2:34390_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HU35KSUBD:RC_AA412041_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
U133_X3P:HS2.104772.1.A1_3P_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U133_PLUS_2:1568611_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U95B:49945_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
HG-U95C:51514_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II hs
MU11KSUBA:U16163_S_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MG-U74AV2:98983_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MG-U74AV2:162188_F_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MOE430A:1417149_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MOUSE430A_2:1417149_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MOUSE430_2:1417149_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide mm
MU19KSUBC:TC39417_S_AT Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide, mRNA (cDNA clone MGC:25333 IMAGE:3157481) mm
RG-U34C:RC_AI175454_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (predicted) rn
RAE230A:1375332_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (predicted) rn
RAT230_2:1375332_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (predicted) rn
RAE230A:1372610_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (predicted) rn
RAT230_2:1372610_AT procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
19538 protein metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5623 cell inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03452 Hypothetical protein Y47D3B.10 [Caenorhabditis elegans] emb|CAA21045.1| Hypothetical protein Y47D3B.10 [Caenorhabditis elegans] ref|NP_499464.1| DumPY : shorter than wild-type family member (dpy-18) [Caenorhabditis elegans] sp|Q10576|P4HA1_CAEEL Prolyl 4-hydroxylase alpha-1 subunit precursor (4-PH alpha-1) (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-1 subunit) emb|CAB71298.1| prolyl 4-hydroxylase alpha subunit 1 [Caenorhabditis elegans] 0.0
blast AAA62207 prolyl 4-hydroxylase alpha subunit 0.0
blast CAE67637 Hypothetical protein CBG13195 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 2.9E-18
Sequence
Target Sequence 
>C. ELEGANS:173395_S_AT
ctgtcccgttcttgtcggaatcaaatgggtatccaacaaatggatccacgagaagggcaa
cgagttccgtcgtccatgcggcttgaaatcctcagaatacgaaagattcgtcggcgacct
cggatacggcccagagccaagaaatgctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTCCCGTTCTTGTCGGAATCAAA 470 239 19 Antisense
GTCCCGTTCTTGTCGGAATCAAATG 697 475 21 Antisense
GTCGGAATCAAATGGGTATCCAACA 62 473 32 Antisense
GGGTATCCAACAAATGGATCCACGA 384 497 45 Antisense
AATGGATCCACGAGAAGGGCAACGA 7 159 57 Antisense
GAGAAGGGCAACGAGTTCCGTCGTC 157 391 68 Antisense
TCCGTCGTCCATGCGGCTTGAAATC 163 593 84 Antisense
GTCCATGCGGCTTGAAATCCTCAGA 407 477 90 Antisense
CGAAAGATTCGTCGGCGACCTCGGA 518 251 118 Antisense
CTCGGATACGGCCCAGAGCCAAGAA 677 231 137 Antisense
CGGCCCAGAGCCAAGAAATGCTCCA 555 241 145 Antisense
Sequence Source Affymetrix Proprietary Database