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[173341_at]

Description CEK115AXR_rc /REP_DB=TREMBL Accession /GB=D73163 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk115a10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 02      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 57      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 294
1 2 3 4 range
A 222.5
(-2.8)
P 		310
(0.7)
P 		367.5
(3)
P 		216.6
(-3)
P 		151
B 234.4
(-1.5)
P 		453.3
(3)
P 		430.6
(2.5)
P 		163.1
(-3)
P 		290
C 308.8
(-2.1)
P 		321.7
(-1.7)
P 		456.9
(3)
P 		283.5
(-3)
P 		173
avr 255.2
(-2) 		361.7
(1.3) 		418.3
(3) 		221.1
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 291.3
(0.8)
P 		276
(0.3)
P 		365.5
(3)
P 		259.4
(-0.2)
P 		220.2
(-1.3)
P 		164.7
(-3)
P 		201
B 418
(3)
P 		333.9
(-0.1)
P 		394.7
(2.1)
P 		313
(-0.9)
P 		257.2
(-3)
P 		258.8
(-2.9)
P 		161
C 343.5
(3)
P 		289.6
(0.5)
P 		234.4
(-2.1)
P 		251.9
(-1.3)
P 		215.2
(-3)
P 		269.6
(-0.5)
P 		128
avr 350.9
(3) 		299.8
(0.4) 		331.5
(2) 		274.8
(-0.8) 		230.9
(-3) 		231
(-3) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 81
(1.6)
P 		67.3
(0.3)
P 		96.1
(3)
P 		31.7
(-3)
P 		64
B 74.8
(0.1)
P 		112
(3)
P 		99.8
(2.1)
P 		34.8
(-3)
P 		77
C 102.2
(-0.1)
P 		92.5
(-0.6)
P 		159.3
(3)
P 		47.8
(-3)
P 		112
avr 86
(0.6) 		90.6
(0.9) 		118.4
(3) 		38.1
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 53.7
(-1.6)
P 		98.2
(3)
P 		79.6
(1.1)
P 		65.8
(-0.3)
P 		66.4
(-0.3)
P 		40.1
(-3)
P 		58
B 145.5
(3)
P 		111.1
(-0.3)
P 		95.8
(-1.8)
P 		82.9
(-3)
P 		104.5
(-0.9)
P 		96.5
(-1.7)
P 		63
C 127.3
(3)
P 		88.4
(-0)
P 		59.7
(-2.3)
P 		50.7
(-3)
P 		69.6
(-1.5)
P 		90.3
(0.1)
P 		77
avr 108.8
(3) 		99.2
(1.6) 		78.4
(-1.3) 		66.5
(-3) 		80.2
(-1.1) 		75.6
(-1.7) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173341_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27755
Sequence Type Exemplar sequence
Representative Public ID CEK115AXR NCBI
Target Description CEK115AXR_rc /REP_DB=TREMBL Accession /GB=D73163 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk115a10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058427 NCBI Caenorhabditis elegans HAlF transporter (PGP related) family member (haf-6) (haf-6) mRNA, complete cds. 11/11 None
SNAP00000040528 ENSEMBL cdna:SNAP chromosome:CEL140:I:1162955:1212987:-1 10/11 None
Y48G8AL.11 ENSEMBL cdna:known chromosome:CEL140:I:1162891:1175870:-1 gene:Y48G8AL.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040546 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1162937-1164703(-) 88.5 88.5
Public Domain and Genome References
Gene Title
Gene Symbol haf-6
Ensembl Y48G8AL.11
Entrez Gene 171694 Entrez gene
SwissProt Q9BL17 EMBL-EBI
WormBase CE30460 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7982.1.S1_AT similar to ATP-binding cassette, sub-family B, member 8 cfa
DROSOPHILA_2:1625618_AT dm
DROSGENOME1:144948_AT dm
HG-U133_PLUS_2:206317_S_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HG-U133A:206317_S_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HG-FOCUS:206317_S_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HG-U133A_2:206317_S_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HU35KSUBA:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HU35KSUBB:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HU35KSUBC:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HU35KSUBD:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HUGENEFL:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HC-G110:HUM_ALU_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
U133_X3P:G9955964_3P_A_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HG-U95AV2:37496_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HU35KSUBD:RC_H49068_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
HG-U95B:42548_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 hs
MOE430A:1422015_A_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOUSE430A_2:1422015_A_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOUSE430_2:1422015_A_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MU11KSUBA:AA606983_S_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MG-U74AV2:104289_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOE430A:1423713_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOUSE430A_2:1423713_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOUSE430_2:1423713_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 mm
MOE430B:1460022_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8, mRNA (cDNA clone MGC:19206 IMAGE:4237894) mm
MOUSE430_2:1460022_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8, mRNA (cDNA clone MGC:19206 IMAGE:4237894) mm
RAE230B:1378041_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 (predicted) rn
RAT230_2:1378041_AT ATP-binding cassette, sub-family B (MDR/TAP), member 8 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29911 Half transporter (pgp related) protein 6 [Caenorhabditis elegans] ref|NP_490828.2| HAlF transporter (PGP related) family member (haf-6) [Caenorhabditis elegans] 0.0
blast CAE63923 Hypothetical protein CBG08495 [Caenorhabditis briggsae] 0.0
blast AAK29911 Half transporter (pgp related) protein 6 [Caenorhabditis elegans] ref|NP_490828.2| HAlF transporter (PGP related) family member (haf-6) [Caenorhabditis elegans] 1.0E-164
blast NP_001017544 hypothetical protein LOC548340 [Danio rerio] gb|AAH92161.1| Hypothetical protein LOC548340 [Danio rerio] 1.0E-158
blast NP_083296 ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Mus musculus] gb|AAH15301.1| ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Mus musculus] dbj|BAE38958.1| unnamed protein product [Mus musculus] dbj|BAC27052.1| unnamed protein product [Mus musculus] dbj|BAB29270.1| unnamed protein product [Mus musculus] 1.0E-157
blast NP_083296 ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Mus musculus] gb|AAH15301.1| ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Mus musculus] dbj|BAE38958.1| unnamed protein product [Mus musculus] dbj|BAC27052.1| unnamed protein product [Mus musculus] dbj|BAB29270.1| unnamed protein product [Mus musculus] 1.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR001140 EMBL-EBI ABC transporter, transmembrane region 2.8E-20
Pfam IPR003439 EMBL-EBI ABC transporter 4.3E-66
Pfam IPR001140 EMBL-EBI ABC transporter, transmembrane region 4.5E-44
Pfam IPR003439 EMBL-EBI ABC transporter 4.3E-66
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490828.2 3 93-115,151-173,230-252
Y48G8AL.11 3 93-115,151-173,230-252
SNAP00000040528 5 144-166,202-224,277-294,298-320,375-397
Sequence
Target Sequence 
>C. ELEGANS:173341_AT
ggattcccattcggagcacatggtacaagaggcgttgaataatgtgatgaagggccgaac
tgtgctcattattgctcatcgattgagcacaattcgatcggctcagatgatttatgtgat
taaggataagaaggcattggaatccggaacccatgagcagttgatggcgaagaaaggatc
gttgtataggaaactggtagaagcacacaatgtggattcttagaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTCCCATTCGGAGCACATGGTA 629 507 73 Antisense
TTCGGAGCACATGGTACAAGAGGCG 702 683 82 Antisense
GAAGGGCCGAACTGTGCTCATTATT 68 335 121 Antisense
GTGCTCATTATTGCTCATCGATTGA 183 479 134 Antisense
GCACAATTCGATCGGCTCAGATGAT 674 317 159 Antisense
GATCGGCTCAGATGATTTATGTGAT 253 419 168 Antisense
GAAGGCATTGGAATCCGGAACCCAT 621 333 202 Antisense
GAATCCGGAACCCATGAGCAGTTGA 596 329 212 Antisense
GAGCAGTTGATGGCGAAGAAAGGAT 361 387 227 Antisense
GGAAACTGGTAGAAGCACACAATGT 523 527 261 Antisense
GCACACAATGTGGATTCTTAGAATA 230 317 275 Antisense
Sequence Source Affymetrix Proprietary Database