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[173325_s_at]

Description AV185177_rc /REP_DB=TREMBL Accession /GB=AV185177 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk674e7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 92      6grp_total > 77      4grp_total > 47      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 290.2
(3)
P 		217.5
(-0.2)
P 		169.6
(-2.3)
P 		152.7
(-3)
P 		138
B 259
(3)
P 		119.1
(-2.1)
P 		93.9
(-3)
P 		233.3
(2.1)
P 		165
C 312
(3)
P 		157.5
(-3)
P 		257.7
(0.9)
P 		158.6
(-3)
P 		155
avr 287.1
(3) 		164.7
(-3) 		173.7
(-2.6) 		181.5
(-2.2) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 287.9
(-2.1)
P 		299.6
(-1.6)
P 		271.1
(-2.8)
P 		266.1
(-3)
P 		327
(-0.5)
P 		414.4
(3)
P 		148
B 268.3
(-2.9)
P 		296.5
(-2.1)
P 		264.6
(-3)
P 		277
(-2.6)
P 		293.3
(-2.2)
P 		467.3
(3)
P 		203
C 286.3
(-2.9)
P 		288
(-2.9)
P 		284.6
(-3)
P 		371.2
(-0.4)
P 		288.6
(-2.9)
P 		483.4
(3)
P 		199
avr 280.8
(-2.8) 		294.7
(-2.3) 		273.4
(-3) 		304.8
(-2) 		303
(-2) 		455
(3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 105.6
(3)
P 		47.2
(-1.2)
P 		44.3
(-1.4)
P 		22.4
(-3)
P 		83
B 82.6
(3)
P 		29.4
(-2.2)
P 		21.7
(-3)
P 		49.7
(-0.2)
P 		61
C 103.3
(3)
P 		45.3
(-1.5)
P 		89.8
(1.9)
P 		26.7
(-3)
P 		77
avr 97.2
(3) 		40.6
(-2.3) 		51.9
(-1.2) 		32.9
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 53.1
(-3)
P 		106.6
(3)
P 		59
(-2.3)
P 		67.5
(-1.4)
P 		98.7
(2.1)
P 		100.9
(2.4)
P 		54
B 93.4
(-1.4)
P 		98.7
(-1.1)
P 		64.3
(-3)
P 		73.4
(-2.5)
P 		119.2
(-0)
P 		174.3
(3)
P 		110
C 106.1
(-0.7)
P 		87.9
(-2)
P 		72.4
(-3)
P 		74.6
(-2.9)
P 		93.4
(-1.6)
P 		161.8
(3)
P 		89
avr 84.2
(-1.6) 		97.7
(-0.6) 		65.2
(-3) 		71.8
(-2.5) 		103.8
(-0.1) 		145.7
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173325_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27880
Sequence Type Exemplar sequence
Representative Public ID AV185177 NCBI
Target Description AV185177_rc /REP_DB=TREMBL Accession /GB=AV185177 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk674e7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058772 NCBI Caenorhabditis elegans P300/CBP Associated Factor homolog family member (pcaf-1) (pcaf-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000034099 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3516554:3523252:1 11/11 None
SNAP00000033984 ENSEMBL cdna:SNAP chromosome:CEL140:I:3518071:3523252:1 11/11 None
Y47G6A.6 ENSEMBL cdna:known chromosome:CEL140:I:3516499:3523368:1 gene:Y47G6A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3522521-3523329(+) 94.14 97.22
Public Domain and Genome References
Gene Title
Gene Symbol pcaf-1
Ensembl Y47G6A.6
Entrez Gene 171920 Entrez gene
SwissProt Q9N3S7 EMBL-EBI
WormBase CE24372 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251849_AT histone acetyltransferase (GCN5) at
CANINE:1604016_AT similar to p300/CBP-associated factor cfa
CANINE:1601529_AT similar to p300/CBP-associated factor cfa
CANINE_2:CFAAFFX.9664.1.S1_AT similar to p300/CBP-associated factor cfa
CHICKEN:GGA.4095.1.S1_AT p300/CBP-associated factor gga
CHICKEN:GGA.4095.2.S1_A_AT p300/CBP-associated factor gga
HG-U95AV2:1012_AT p300/CBP-associated factor hs
HC-G110:1012_AT p300/CBP-associated factor hs
HU35KSUBB:RC_N70861_AT p300/CBP-associated factor hs
HUGENEFL:U57317_AT p300/CBP-associated factor hs
U133_X3P:HS.199061.0.A2_3P_AT p300/CBP-associated factor hs
HG-U133A_2:203845_AT p300/CBP-associated factor hs
HG-FOCUS:203845_AT p300/CBP-associated factor hs
HG-U133_PLUS_2:203845_AT p300/CBP-associated factor hs
HG-U133A:203845_AT p300/CBP-associated factor hs
HG-U95C:63536_AT p300/CBP-associated factor hs
HG-U95C:63537_G_AT p300/CBP-associated factor hs
HG-U95C:63868_AT p300/CBP-associated factor hs
HG-U95E:69300_AT p300/CBP-associated factor hs
HG-U133_PLUS_2:239585_AT P300/CBP-associated factor hs
HG-U133B:239585_AT P300/CBP-associated factor hs
HG-U95C:62840_AT P300/CBP-associated factor hs
HG-U95E:70152_R_AT P300/CBP-associated factor hs
HG-U95E:75936_AT P300/CBP-associated factor hs
MOUSE430_2:1450821_AT p300/CBP-associated factor mm
MOE430A:1450821_AT p300/CBP-associated factor mm
MOUSE430A_2:1450821_AT p300/CBP-associated factor mm
MG-U74AV2:104070_AT p300/CBP-associated factor mm
MG-U74AV2:161116_AT p300/CBP-associated factor mm
MOE430A:1434037_S_AT p300/CBP-associated factor mm
MOUSE430A_2:1434037_S_AT p300/CBP-associated factor mm
MOUSE430_2:1434037_S_AT p300/CBP-associated factor mm
MOE430B:1457809_AT p300/CBP-associated factor mm
MOUSE430_2:1457809_AT p300/CBP-associated factor mm
MOE430B:1444473_AT P300/CBP-associated factor (Pcaf), mRNA mm
MOUSE430_2:1444473_AT P300/CBP-associated factor (Pcaf), mRNA mm
MOE430B:1445105_AT P300/CBP-associated factor (Pcaf), mRNA mm
MOUSE430_2:1445105_AT P300/CBP-associated factor (Pcaf), mRNA mm
MU19KSUBB:TC32704_AT P300/CBP-associated factor (Pcaf), mRNA mm
MU19KSUBC:TC40339_AT P300/CBP-associated factor (Pcaf), mRNA mm
MU19KSUBC:TC40339_G_AT P300/CBP-associated factor (Pcaf), mRNA mm
RG-U34C:RC_AI105431_AT p300/CBP-associated factor (predicted) rn
RAE230A:1375205_AT p300/CBP-associated factor (predicted) rn
RAT230_2:1375205_AT p300/CBP-associated factor (predicted) rn
RAE230B:1393762_AT p300/CBP-associated factor (predicted) rn
RAT230_2:1393762_AT p300/CBP-associated factor (predicted) rn
YEAST_2:1771978_AT Histone acetyltransferase, acetylates lysine 14 on histone H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8080 N-acetyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491173 P300/CBP Associated Factor homolog family member (pcaf-1) [Caenorhabditis elegans] gb|AAF60658.1| P300/cbp associated factor homolog protein 1 [Caenorhabditis elegans] 0.0
blast CAE68959 Hypothetical protein CBG14939 [Caenorhabditis briggsae] 0.0
blast AAC50890 p300/CBP-associated factor [Homo sapiens] sp|Q92831|PCAF_HUMAN Histone acetylatransferase PCAF (P300/CBP-associated factor) (P/CAF) (Histone acetylase PCAF) 8.0E-82
blast AAC50890 p300/CBP-associated factor [Homo sapiens] sp|Q92831|PCAF_HUMAN Histone acetylatransferase PCAF (P300/CBP-associated factor) (P/CAF) (Histone acetylase PCAF) 1.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR000182 EMBL-EBI GCN5-related N-acetyltransferase 9.4E-14
Pfam IPR001487 EMBL-EBI Bromodomain 7.3E-30
Pfam IPR009464 EMBL-EBI PCAF, N-terminal 1.0E-126
Pfam IPR000182 EMBL-EBI GCN5-related N-acetyltransferase 3.3E-8
Pfam IPR001487 EMBL-EBI Bromodomain 7.3E-30
Pfam IPR000182 EMBL-EBI GCN5-related N-acetyltransferase 9.4E-14
Pfam IPR001487 EMBL-EBI Bromodomain 7.3E-30
Pfam IPR009464 EMBL-EBI PCAF, N-terminal 3.9E-47
Sequence
Target Sequence 
>C. ELEGANS:173325_S_AT
acggaaggcttatactcatcaacacttgttcatagccgacctgaatcgcctcttccaaaa
ctgttacgtgttcaacggagctgaagctgtctactataaatatggctataaactnnnnnn
nnnnnnnnnnnnnnntctgaaaacgtcgttcccggagagctcgtggtatccagagctccc
c

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGAAGGCTTATACTCATCAACAC 13 95 50 Antisense
GGCTTATACTCATCAACACTTGTTC 221 537 56 Antisense
ATCAACACTTGTTCATAGCCGACCT 560 25 67 Antisense
CTTGTTCATAGCCGACCTGAATCGC 604 217 74 Antisense
GAATCGCCTCTTCCAAAACTGTTAC 508 327 92 Antisense
GCCTCTTCCAAAACTGTTACGTGTT 599 279 97 Antisense
AACTGTTACGTGTTCAACGGAGCTG 204 141 108 Antisense
GGAGCTGAAGCTGTCTACTATAAAT 700 521 126 Antisense
TGAAAACGTCGTTCCCGGAGAGCTC 420 567 187 Antisense
TTCCCGGAGAGCTCGTGGTATCCAG 57 685 198 Antisense
GAGCTCGTGGTATCCAGAGCTCCCC 215 387 206 Antisense
Sequence Source Affymetrix Proprietary Database