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[173321_at] endoplasmin precursor (GRP94)

Description CEK001C3R_rc /REP_DB=TREMBL Accession /GB=D27275 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk1c3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 31      4grp_highest_8_8 > 09      4grp_high_8_8 > 54      6grp_highest_nonorm > 23      6grp_total > 43      4grp_total > 58      6grp_high1_sub > 03      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2786
1 2 3 4 range
A 1576.8
(-0.7)
P 		2671.8
(3)
P 		2472.6
(2.3)
P 		900.5
(-3)
P 		1771
B 1529.2
(-2.3)
P 		2903.1
(1.1)
P 		3686.7
(3)
P 		1238
(-3)
P 		2449
C 1820.6
(-0.6)
P 		2976.1
(2.9)
P 		3025.3
(3)
P 		1043.8
(-3)
P 		1982
avr 1642.2
(-1.3) 		2850.3
(2.4) 		3061.5
(3) 		1060.8
(-3) 		2001

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1542
1 2 3 4 5 6 range
A 1493.4
(-2.4)
P 		2198.4
(0.4)
P 		2866.5
(3)
P 		1614.3
(-2)
P 		1543
(-2.2)
P 		1351.1
(-3)
P 		1515
B 2337.8
(0.9)
P 		2362.5
(1)
P 		2875.7
(3)
P 		1730.5
(-1.5)
P 		1515.5
(-2.3)
P 		1333.6
(-3)
P 		1542
C 1904.1
(0.9)
P 		2166.3
(2.8)
P 		2195.2
(3)
P 		1486.5
(-2.2)
P 		1556.2
(-1.7)
P 		1381.7
(-3)
P 		814
avr 1911.8
(-0.4) 		2242.4
(1.1) 		2645.8
(3) 		1610.4
(-1.8) 		1538.2
(-2.2) 		1355.5
(-3) 		1290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 923
1 2 3 4 range
A 574.1
(2.2)
P 		580
(2.2)
P 		646.4
(3)
P 		131.8
(-3)
P 		515
B 487.7
(-0.7)
P 		717
(1.6)
P 		854.3
(3)
P 		263.8
(-3)
P 		591
C 602.7
(-0.1)
P 		855.6
(1.6)
P 		1054.4
(3)
P 		176
(-3)
P 		878
avr 554.8
(0.3) 		717.5
(1.8) 		851.7
(3) 		190.5
(-3) 		661

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 538
1 2 3 4 5 6 range
A 275.3
(-3)
P 		781.8
(3)
P 		624.3
(1.1)
P 		409.4
(-1.4)
P 		465.6
(-0.7)
P 		328.9
(-2.4)
P 		507
B 813.4
(3)
P 		786.4
(2.5)
P 		698.3
(1.1)
P 		458.4
(-3)
P 		615.7
(-0.3)
P 		497.3
(-2.3)
P 		355
C 705.8
(3)
P 		661
(2.3)
P 		558.7
(0.8)
P 		298.9
(-3)
P 		503.6
(0)
P 		462.5
(-0.6)
P 		407
avr 598.2
(0.5) 		743.1
(3) 		627.1
(1) 		388.9
(-3) 		528.3
(-0.6) 		429.6
(-2.3) 		354
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173321_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27674
Sequence Type Exemplar sequence
Representative Public ID CEK001C3R NCBI
Target Description CEK001C3R_rc /REP_DB=TREMBL Accession /GB=D27275 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk1c3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069679
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000031874 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11119423:11122910:1 None
T05E11.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:11119333:11123126:1 gene:T05E11.3 A 
SNAP00000031863 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11119423:11122910:1 None
T05E11.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:11119347:11123097:1 gene:T05E11.3 A 
NM_069679 NCBI Caenorhabditis elegans T05E11.3 (T05E11.3) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11122691-11123114(+) 82.83 82.83
Public Domain and Genome References
Gene Title endoplasmin precursor (GRP94)
Ensembl T05E11.3
Entrez Gene 178014 Entrez gene
SwissProt Q22235 EMBL-EBI
WormBase CE06362 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16448_G_AT shepherd protein (SHD) / clavata formation protein, putative at
ATGENOME1:16447_AT shepherd protein (SHD) / clavata formation protein, putative at
ATH1-121501:254166_AT shepherd protein (SHD) / clavata formation protein, putative at
CANINE:1582369_AT tumor rejection antigen (gp96) 1 cfa
CANINE_2:CFA.3896.1.S1_S_AT tumor rejection antigen (gp96) 1 cfa
CANINE_2:CFAAFFX.12023.1.S1_AT tumor rejection antigen (gp96) 1 cfa
DROSOPHILA_2:1636528_AT Glycoprotein 93 dm
DROSGENOME1:154182_AT Glycoprotein 93 dm
CHICKEN:GGA.4724.1.S1_AT tumor rejection antigen (gp96) 1 gga
CHICKEN:GGA.4724.1.S2_AT tumor rejection antigen (gp96) 1 gga
HG-U133_PLUS_2:200599_S_AT tumor rejection antigen (gp96) 1 hs
HG-U133A:200599_S_AT tumor rejection antigen (gp96) 1 hs
HG-FOCUS:200599_S_AT tumor rejection antigen (gp96) 1 hs
HG-U133A_2:200599_S_AT tumor rejection antigen (gp96) 1 hs
HG-U95AV2:442_AT tumor rejection antigen (gp96) 1 hs
HC-G110:442_AT tumor rejection antigen (gp96) 1 hs
HG-U133_PLUS_2:200598_S_AT tumor rejection antigen (gp96) 1 hs
HG-U133A_2:200598_S_AT tumor rejection antigen (gp96) 1 hs
HG-U133A:200598_S_AT tumor rejection antigen (gp96) 1 hs
HU35KSUBA:RC_D51235_F_AT tumor rejection antigen (gp96) 1 hs
HU35KSUBC:RC_R20669_F_AT tumor rejection antigen (gp96) 1 hs
HU35KSUBC:RC_H99801_S_AT tumor rejection antigen (gp96) 1 hs
HUGENEFL:X15187_AT tumor rejection antigen (gp96) 1 hs
HG-U133_PLUS_2:216449_X_AT tumor rejection antigen (gp96) 1 hs
HG-U133A_2:216449_X_AT tumor rejection antigen (gp96) 1 hs
HG-U133A:216449_X_AT tumor rejection antigen (gp96) 1 hs
U133_X3P:G4507676_3P_A_AT tumor rejection antigen (gp96) 1 hs
U133_X3P:HS.82689.0.S1_3P_A_AT tumor rejection antigen (gp96) 1 hs
U133_X3P:HS.288528.0.S1_3P_S_AT tumor rejection antigen (gp96) 1 hs
U133_X3P:216449_3P_X_AT tumor rejection antigen (gp96) 1 hs
HG-U133_PLUS_2:216450_X_AT tumor rejection antigen (gp96) 1 hs
HG-U133A_2:216450_X_AT tumor rejection antigen (gp96) 1 hs
HG-U133A:216450_X_AT tumor rejection antigen (gp96) 1 hs
U133_X3P:HS.192374.0.S1_3P_AT Tumor rejection antigen (gp96) 1 hs
HG-U133_PLUS_2:239451_AT Tumor rejection antigen (gp96) 1 hs
HG-U133B:239451_AT Tumor rejection antigen (gp96) 1 hs
HG-U95E:68624_AT Tumor rejection antigen (gp96) 1 hs
MOUSE430_2:1415889_A_AT tumor rejection antigen gp96 mm
MOUSE430A_2:1415889_A_AT tumor rejection antigen gp96 mm
MOE430A:1415889_A_AT tumor rejection antigen gp96 mm
MOUSE430A_2:1438040_A_AT tumor rejection antigen gp96 mm
MOUSE430_2:1438040_A_AT tumor rejection antigen gp96 mm
MOE430A:1438040_A_AT tumor rejection antigen gp96 mm
MU11KSUBB:MSA.27657.0_F_AT tumor rejection antigen gp96 mm
MU11KSUBB:MSA.11833.0_F_AT tumor rejection antigen gp96 mm
MU11KSUBB:MSA.12687.0_S_AT tumor rejection antigen gp96 mm
MU11KSUBB:MSA.10652.0_F_AT tumor rejection antigen gp96 mm
MG-U74AV2:160499_AT tumor rejection antigen gp96 mm
MG-U74CV2:167108_R_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
MU19KSUBA:TC15686_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
MU19KSUBA:TC16545_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
MU19KSUBA:TC16597_S_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
MU19KSUBB:TC29664_S_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
MU19KSUBB:TC33238_AT Tumor rejection antigen gp96, mRNA (cDNA clone MGC:6208 IMAGE:3587178) mm
RT-U34:AA685903_AT tumor rejection antigen gp96 (predicted) rn
RG-U34A:AA685903_AT tumor rejection antigen gp96 (predicted) rn
RG-U34A:S69316_S_AT tumor rejection antigen gp96 (predicted) rn
RG-U34A:S69315_AT tumor rejection antigen gp96 (predicted) rn
RG-U34B:RC_AA963782_AT tumor rejection antigen gp96 (predicted) rn
RG-U34C:RC_AI230121_AT tumor rejection antigen gp96 (predicted) rn
RAE230A:1388331_AT tumor rejection antigen gp96 (predicted) rn
RAT230_2:1388331_AT tumor rejection antigen gp96 (predicted) rn
RG-U34C:RC_AI101926_AT Tumor rejection antigen gp96 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92973 Hypothetical protein T05E11.3 [Caenorhabditis elegans] ref|NP_502080.1| T05E11.3 [Caenorhabditis elegans] 0.0
blast CAE62006 Hypothetical protein CBG06014 [Caenorhabditis briggsae] 0.0
blast XP_576206 PREDICTED: tumor rejection antigen gp96 (predicted) [Rattus norvegicus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001404 EMBL-EBI Heat shock protein Hsp90 1.0E-126
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 1.3E-13
Sequence
Target Sequence 
>C. ELEGANS:173321_AT
cttgccaagcttcctctttcgaagcttgcnactccttcgttctgggattctcaacttcaa
gatcaaagttggttttgctgaaagaatcggaagctgtccttcgccaatcgctcgatgttt
cccaannnnnncaagtggaaccggaccagcatatggaggggntgaaccnggacccagnan
ccgntggagaaacaacgattgaggaggagcactctgagctctaaacaatgtaaaacatct
ccccattccattagctttatgcactcgatgatattgtgataagataagataattacgtga
ngggtatggacgagaagcatgannnntccccggacttctcagtctcagtacttctcgaac
actttacggt

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGCCAAGCTTCCTCTTTCGAAGC 434 219 22 Antisense
CTTCGTTCTGGGATTCTCAACTTCA 199 221 56 Antisense
GAAAGAATCGGAAGCTGTCCTTCGC 543 359 101 Antisense
CAAGTGGAACCGGACCAGCATATGG 619 181 153 Antisense
GGAACCGGACCAGCATATGGAGGGG 498 527 158 Antisense
CACTCTGAGCTCTAAACAATGTAAA 593 195 231 Antisense
AATGTAAAACATCTCCCCATTCCAT 502 163 248 Antisense
CTCCCCATTCCATTAGCTTTATGCA 228 235 260 Antisense
AGCTTTATGCACTCGATGATATTGT 290 79 274 Antisense
TTCTCAGTCTCAGTACTTCTCGAAC 694 691 357 Antisense
CAGTACTTCTCGAACACTTTACGGT 508 205 367 Antisense
Sequence Source Affymetrix Proprietary Database