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[173314_at]

Description CEK002BXR_rc /REP_DB=TREMBL Accession /GB=D27057 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk2b10 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 47      6grp_total > 58      4grp_total > 43      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 131
(-0.5)
P 		138.3
(3)
M 		125.8
(-3)
P 		137.8
(2.8)
A 		13
B 123.1
(2.5)
P 		127.7
(3)
A 		123.3
(2.5)
P 		75.5
(-3)
P 		52
C 123.2
(-3)
A 		157.5
(3)
A 		134.9
(-1)
P 		157
(2.9)
P 		34
avr 125.8
(-2.2) 		141.2
(3) 		128
(-1.4) 		123.4
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 130
(0.5)
P 		113.1
(-0.3)
P 		82.7
(-1.7)
A 		66.1
(-2.5)
P 		54.5
(-3)
P 		184.9
(3)
P 		130
B 45.9
(-3)
A 		107
(2.2)
P 		112
(2.6)
A 		86
(0.4)
A 		107.1
(2.2)
A 		116.9
(3)
P 		71
C 183.7
(3)
P 		60.7
(-3)
A 		143.9
(1.1)
P 		82.9
(-1.9)
A 		153.4
(1.5)
P 		150.7
(1.4)
P 		123
avr 119.9
(0.4) 		93.6
(-1.7) 		112.9
(-0.1) 		78.3
(-3) 		105
(-0.8) 		150.8
(3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 47.7
(3)
P 		30
(-0.9)
M 		32.9
(-0.2)
P 		20.2
(-3)
A 		28
B 39.2
(3)
P 		31.5
(1)
A 		28.6
(0.2)
P 		16.1
(-3)
P 		23
C 40.8
(1.2)
A 		45.3
(2.5)
A 		47
(3)
P 		26.5
(-3)
P 		21
avr 42.6
(3) 		35.6
(1.1) 		36.2
(1.2) 		20.9
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 24
(-1.4)
P 		40.2
(2)
P 		18
(-2.7)
A 		16.8
(-2.9)
P 		16.4
(-3)
P 		45
(3)
P 		29
B 16
(-3)
A 		35.6
(1.3)
P 		27.2
(-0.6)
A 		22.8
(-1.5)
A 		43.5
(3)
A 		43.6
(3)
P 		28
C 68.1
(3)
P 		18.5
(-2.8)
A 		36.6
(-0.7)
P 		16.7
(-3)
A 		49.6
(0.8)
P 		50.5
(0.9)
P 		51
avr 36
(0.7) 		31.4
(-0.3) 		27.3
(-1.2) 		18.8
(-3) 		36.5
(0.8) 		46.4
(3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173314_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27686
Sequence Type Exemplar sequence
Representative Public ID CEK002BXR NCBI
Target Description CEK002BXR_rc /REP_DB=TREMBL Accession /GB=D27057 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk2b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058433(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058433 NCBI Caenorhabditis elegans Y48G8AL.1 (Y48G8AL.1) mRNA, complete cds. 10/11 None
GENEFINDER00000040547 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1231590:1254500:1 10/11 None
SNAP00000040537 ENSEMBL cdna:SNAP chromosome:CEL140:I:1253340:1254500:1 10/11 None
Y48G8AL.1 ENSEMBL cdna:known chromosome:CEL140:I:1211494:1254666:1 gene:Y48G8AL.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1253345-1254635(+) 95.81 95.81
Public Domain and Genome References
Gene Title
Ensembl Y48G8AL.1
Entrez Gene 171700 Entrez gene
SwissProt Q9BL11 EMBL-EBI
WormBase CE29116 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.15354.1.S1_S_AT similar to HECT domain and RCC1-like domain protein 3 cfa
CANINE_2:CFAAFFX.15357.1.S1_S_AT similar to HECT domain and RCC1-like domain protein 3 cfa
MG-U74BV2:114779_AT hect domain and RLD 3 mm
MOE430B:1431202_AT hect domain and RLD 3 mm
MOUSE430_2:1431202_AT hect domain and RLD 3 mm
MOE430B:1433590_AT hect domain and RLD 3 mm
MOUSE430_2:1433590_AT hect domain and RLD 3 mm
MU19KSUBA:TC21541_AT Hect domain and RLD 3 (Herc3), mRNA mm
MU19KSUBB:TC27755_AT Hect domain and RLD 3 (Herc3), mRNA mm
RG-U34B:RC_AA943020_AT hect domain and RLD 3 (predicted) rn
RAE230A:1389164_AT hect domain and RLD 3 (predicted) rn
RAT230_2:1389164_AT hect domain and RLD 3 (predicted) rn
RG-U34C:RC_AI101116_AT Hect domain and RLD 3 (predicted) rn
RAE230A:1375861_AT Hect domain and RLD 3 (predicted) rn
RAT230_2:1375861_AT Hect domain and RLD 3 (predicted) rn
RAE230B:1380046_AT Hect domain and RLD 3 (predicted) rn
RAT230_2:1380046_AT Hect domain and RLD 3 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
4869 cysteine protease inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29908 Hypothetical protein Y48G8AL.1 [Caenorhabditis elegans] ref|NP_490834.1| Y48G8AL.1 [Caenorhabditis elegans] 0.0
blast CAE63916 Hypothetical protein CBG08488 [Caenorhabditis briggsae] 0.0
blast CAH65316 hypothetical protein [Gallus gallus] 1.0E-158
blast XP_795107 PREDICTED: similar to hect domain and RLD 4 isoform b [Strongylocentrotus purpuratus] 1.0E-83
blast AAK29908 Hypothetical protein Y48G8AL.1 [Caenorhabditis elegans] ref|NP_490834.1| Y48G8AL.1 [Caenorhabditis elegans] 5.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 9.3E-10
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 3.4E-5
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 4.7E-41
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 5.1E-31
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 7.0E-19
Sequence
Target Sequence 
>C. ELEGANS:173314_AT
gaggcgaatactcatcatctcacccgacaattcaagcannnnnnnnnnnnnnnnncgcgt
tgaccgacgatgagcgcagaaagtttcttcaannnttgacgggctccacgcgaattccag
tttccggatgggaagagcttcacgcggcaattcagccatccgcgccagaatcactcccag
tcgcccatacatgcttcaaccttctggatcttcctaacatcgcagacgacgtcgagctgc
tccgccgcctccgcatctccattgagcacaccgaaggcttcacccttgcctagatattaa
t

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGCGAATACTCATCATCTCACCC 565 405 14 Antisense
CGATGAGCGCAGAAAGTTTCTTCAA 530 249 81 Antisense
TTCCAGTTTCCGGATGGGAAGAGCT 577 699 128 Antisense
ATGGGAAGAGCTTCACGCGGCAATT 68 51 141 Antisense
TTCACGCGGCAATTCAGCCATCCGC 457 683 152 Antisense
TTCAACCTTCTGGATCTTCCTAACA 547 685 208 Antisense
ATCTTCCTAACATCGCAGACGACGT 466 29 221 Antisense
CATCGCAGACGACGTCGAGCTGCTC 573 209 231 Antisense
CATCTCCATTGAGCACACCGAAGGC 668 211 267 Antisense
CATTGAGCACACCGAAGGCTTCACC 558 217 273 Antisense
GCTTCACCCTTGCCTAGATATTAAT 362 303 290 Antisense
Sequence Source Affymetrix Proprietary Database