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[173306_s_at] S.pombe mitotic control protein like

Description CEC8833_rc /REP_DB=TREMBL Accession /GB=C10883 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk159a10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 55      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 82      6grp_total > 56      4grp_total > 62      6grp_cgc > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 430.8
(3)
P 		373.8
(0.1)
P 		351.9
(-1)
P 		311.6
(-3)
P 		119
B 400.4
(1.9)
P 		344.2
(-3)
P 		413.1
(3)
P 		363.2
(-1.3)
P 		69
C 435.4
(3)
P 		310
(-0.8)
P 		423.3
(2.6)
P 		239
(-3)
P 		196
avr 422.2
(3) 		342.7
(-1.1) 		396.1
(1.7) 		304.6
(-3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 293
1 2 3 4 5 6 range
A 419.4
(2.2)
P 		317.4
(-0.7)
P 		449
(3)
P 		236.2
(-3)
P 		289.4
(-1.5)
P 		339.7
(-0.1)
P 		213
B 452.2
(1.5)
P 		449.2
(1.4)
P 		419.5
(0.7)
P 		398.5
(0.3)
P 		249.3
(-3)
P 		522.6
(3)
P 		273
C 229.2
(-3)
P 		409.6
(1.1)
P 		235.1
(-2.9)
P 		420.9
(1.3)
P 		258.4
(-2.3)
P 		495.9
(3)
P 		267
avr 366.9
(0.2) 		392.1
(1.1) 		367.9
(0.3) 		351.9
(-0.2) 		265.7
(-3) 		452.7
(3) 		187
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 156.9
(3)
P 		81.1
(-1.1)
P 		92
(-0.5)
P 		45.6
(-3)
P 		111
B 127.7
(3)
P 		85
(-2.1)
P 		95.7
(-0.8)
P 		77.4
(-3)
P 		50
C 144.1
(2.8)
P 		89.1
(-0.3)
P 		147.5
(3)
P 		40.3
(-3)
P 		107
avr 142.9
(3) 		85.1
(-0.9) 		111.7
(0.9) 		54.4
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 77.3
(-1)
P 		112.9
(3)
P 		97.8
(1.3)
P 		59.9
(-3)
P 		87.3
(0.1)
P 		82.7
(-0.4)
P 		53
B 157.3
(0.6)
P 		149.5
(0.1)
P 		101.9
(-3)
P 		105.6
(-2.7)
P 		101.3
(-3)
P 		194.9
(3)
P 		94
C 84.9
(-1.6)
P 		125
(0.7)
P 		59.8
(-3)
P 		84.7
(-1.6)
P 		83.6
(-1.7)
P 		166
(3)
P 		106
avr 106.5
(-0.9) 		129.1
(1.3) 		86.5
(-2.7) 		83.4
(-3) 		90.7
(-2.3) 		147.9
(3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173306_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27810
Sequence Type Exemplar sequence
Representative Public ID CEC8833 NCBI
Target Description CEC8833_rc /REP_DB=TREMBL Accession /GB=C10883 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk159a10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069434(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069434 NCBI Caenorhabditis elegans DIS3 (yeast disjunction abnormal) exonuclease homolog family member (dis-3) (dis-3) mRNA, complete cds. 11/11 A 
SNAP00000040136 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10103261:10106343:1 11/11 None
GENEFINDER00000040151 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10103151:10106343:1 11/11 None
C04G2.6 ENSEMBL cdna:known chromosome:CEL140:IV:10097180:10106528:1 gene:C04G2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10105868-10106381(+) 80.67 80.67
Public Domain and Genome References
Gene Title S.pombe mitotic control protein like
Gene Symbol dis-3
Ensembl C04G2.6
Entrez Gene 177875 Entrez gene
SwissProt Q17632 EMBL-EBI
WormBase CE05214 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:19554_AT ribonuclease II family protein at
ATH1-121501:263077_AT ribonuclease II family protein at
CANINE:1605753_AT similar to KIAA1008 cfa
CANINE_2:CFAAFFX.8460.1.S1_S_AT similar to KIAA1008 cfa
DROSOPHILA_2:1637153_AT dm
DROSGENOME1:141818_AT dm
CHICKEN:GGAAFFX.25134.1.S1_S_AT similar to KIAA1008; mitotic control protein dis3 homolog; 2810028N01Rik gga
HG-U133_PLUS_2:234660_S_AT KIAA1008 hs
HG-U133B:234660_S_AT KIAA1008 hs
HG-U133_PLUS_2:218362_S_AT KIAA1008 hs
HG-U133A:218362_S_AT KIAA1008 hs
HG-U133A_2:218362_S_AT KIAA1008 hs
HG-U133_PLUS_2:222607_S_AT KIAA1008 hs
HG-U133B:222607_S_AT KIAA1008 hs
HG-U133_PLUS_2:214194_AT KIAA1008 hs
HG-U133A_2:214194_AT KIAA1008 hs
HG-U133A:214194_AT KIAA1008 hs
HU35KSUBB:RC_R15846_AT KIAA1008 hs
HUGENEFL:U79246_AT KIAA1008 hs
U133_X3P:G7662443_3P_X_AT KIAA1008 hs
U133_X3P:HS.323346.1.S1_3P_A_AT KIAA1008 hs
U133_X3P:HS.323346.0.S1_3P_A_AT KIAA1008 hs
U133_X3P:HS.179566.0.S1_3P_AT KIAA1008 hs
U133_X3P:218362_3P_S_AT KIAA1008 hs
HG-U95AV2:40633_AT KIAA1008 hs
HG-U133_PLUS_2:236560_AT KIAA1008 hs
HG-U133B:236560_AT KIAA1008 hs
HU35KSUBB:RC_R23924_AT KIAA1008 hs
HU35KSUBC:RC_W92248_AT KIAA1008 hs
U133_X3P:HS.59661.0.S1_3P_AT KIAA1008 hs
U133_X3P:HS.326725.0.A1_3P_AT KIAA1008 hs
HG-U133_PLUS_2:234757_AT KIAA1008 hs
HG-U133B:234757_AT KIAA1008 hs
HU35KSUBD:RC_AA258601_AT KIAA1008 hs
MG-U74BV2:164017_AT RIKEN cDNA 2810028N01 gene mm
MOE430A:1426609_AT RIKEN cDNA 2810028N01 gene mm
MOUSE430A_2:1426609_AT RIKEN cDNA 2810028N01 gene mm
MOUSE430_2:1426609_AT RIKEN cDNA 2810028N01 gene mm
MU19KSUBC:TC41415_AT RIKEN cDNA 2810028N01 gene mm
MU19KSUBC:TC41465_AT RIKEN cDNA 2810028N01 gene, mRNA (cDNA clone MGC:37123 IMAGE:4952415) mm
YEAST_2:1770063_AT Nucleolar exosome component, involved in rRNA processing and RNA degradation, binds Gsp1p/Ran and enhances the GEF activity of Srm1p, implicated in mitotic control, homologous to the E. coli RNase R of the RNase II family Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
4540 ribonuclease activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94677 Hypothetical protein C04G2.6 [Caenorhabditis elegans] ref|NP_501835.1| DIS3 (yeast disjunction abnormal) exonuclease homolog family member (dis-3) [Caenorhabditis elegans] sp|Q17632|RRP44_CAEEL Probable exosome complex exonuclease RRP44 (Ribosomal RNA processing protein 44) (DIS3 protein homolog) 0.0
blast CAE67902 Hypothetical protein CBG13499 [Caenorhabditis briggsae] 0.0
blast EAA09476 ENSANGP00000003705 [Anopheles gambiae str. PEST] ref|XP_314089.2| ENSANGP00000003705 [Anopheles gambiae str. PEST] 1.0E-175
blast CAA94677 Hypothetical protein C04G2.6 [Caenorhabditis elegans] ref|NP_501835.1| DIS3 (yeast disjunction abnormal) exonuclease homolog family member (dis-3) [Caenorhabditis elegans] sp|Q17632|RRP44_CAEEL Probable exosome complex exonuclease RRP44 (Ribosomal RNA processing protein 44) (DIS3 protein homolog) 1.0E-167
blast CAE67902 Hypothetical protein CBG13499 [Caenorhabditis briggsae] 1.0E-112
blast CAA94677 Hypothetical protein C04G2.6 [Caenorhabditis elegans] ref|NP_501835.1| DIS3 (yeast disjunction abnormal) exonuclease homolog family member (dis-3) [Caenorhabditis elegans] sp|Q17632|RRP44_CAEEL Probable exosome complex exonuclease RRP44 (Ribosomal RNA processing protein 44) (DIS3 protein homolog) 1.0E-105
blast CAE67902 Hypothetical protein CBG13499 [Caenorhabditis briggsae] 1.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR001900 EMBL-EBI Ribonuclease II 1.0E-126
Pfam IPR001900 EMBL-EBI Ribonuclease II 1.7E-7
Sequence
Target Sequence 
>C. ELEGANS:173306_S_AT
agcttctggtacaactatcgatgtagaggaaatgtcggtcacagtcaatggagatgttgt
tatcaaagaattggaaccggtaactgtgcgaatcagtgtgaacgagnnnnnccaacaacg
tccacgtgtcgaacttcagctaattaaaccagccattccaggactctctgttgattttga
tctttcttcttctgaaggccttggacttta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTTCTGGTACAACTATCGATGTA 557 77 14 Antisense
GTCAATGGAGATGTTGTTATCAAAG 308 463 57 Antisense
ATTGGAACCGGTAACTGTGCGAATC 207 3 83 Antisense
GTAACTGTGCGAATCAGTGTGAACG 504 461 93 Antisense
CCAACAACGTCCACGTGTCGAACTT 476 273 125 Antisense
TCCACGTGTCGAACTTCAGCTAATT 196 589 134 Antisense
TCAGCTAATTAAACCAGCCATTCCA 570 623 149 Antisense
ATTAAACCAGCCATTCCAGGACTCT 329 11 156 Antisense
GCCATTCCAGGACTCTCTGTTGATT 491 281 165 Antisense
TGATCTTTCTTCTTCTGAAGGCCTT 254 565 191 Antisense
CTTCTTCTGAAGGCCTTGGACTTTA 479 219 199 Antisense
Sequence Source Affymetrix Proprietary Database