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[173305_s_at]

Description CEK053C5R_rc /REP_DB=TREMBL Accession /GB=D64491 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk53c5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 31      4grp_high_8_8 > 20      6grp_highest_nonorm > 68      6grp_total > 49      4grp_total > 19      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2245
1 2 3 4 range
A 2446.1
(-1)
P 		3121.9
(0.9)
P 		1717
(-3)
P 		3899.3
(3)
P 		2182
B 2171.8
(-1.1)
P 		2249.6
(-0.9)
P 		1654
(-3)
P 		3318
(3)
P 		1664
C 2084.9
(-2)
P 		1955.9
(-2.6)
P 		1845.3
(-3)
P 		3349.3
(3)
P 		1504
avr 2234.3
(-1.3) 		2442.5
(-0.6) 		1738.8
(-3) 		3522.2
(3) 		1783

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1881
1 2 3 4 5 6 range
A 2855.6
(1.6)
P 		2325
(-0.1)
P 		1415.4
(-3)
P 		1500.5
(-2.7)
P 		2532.9
(0.6)
P 		3296.2
(3)
P 		1881
B 2502.9
(0.6)
P 		2266.8
(-0.4)
P 		1602.4
(-3)
P 		1900.1
(-1.8)
P 		2139.6
(-0.9)
P 		3111.2
(3)
P 		1509
C 2600.5
(1.2)
P 		2250.6
(-0.8)
P 		1870
(-3)
P 		1927.2
(-2.7)
P 		2441.8
(0.3)
P 		2923
(3)
P 		1053
avr 2653
(1.1) 		2280.8
(-0.4) 		1629.3
(-3) 		1775.9
(-2.4) 		2371.4
(0) 		3110.1
(3) 		1481
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 507
1 2 3 4 range
A 890.6
(3)
P 		677.7
(0.1)
P 		448.8
(-3)
P 		570.8
(-1.3)
P 		442
B 692.6
(2.7)
P 		555.6
(0.2)
P 		383.3
(-3)
P 		707.1
(3)
P 		324
C 690.1
(3)
P 		562.3
(-3)
P 		643.2
(0.8)
P 		564.7
(-2.9)
P 		128
avr 757.8
(3) 		598.5
(-0.6) 		491.8
(-3) 		614.2
(-0.2) 		266

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 852
1 2 3 4 5 6 range
A 526.4
(-0.5)
P 		826.9
(3)
P 		308.2
(-3)
P 		380.6
(-2.2)
P 		764.3
(2.3)
P 		802.5
(2.7)
P 		519
B 870.9
(0.7)
P 		754.5
(-0.2)
P 		389.1
(-3)
P 		503.3
(-2.1)
P 		869.3
(0.7)
P 		1160.2
(3)
P 		771
C 963.9
(2.9)
P 		686.7
(0)
P 		475.9
(-2.1)
P 		387.6
(-3)
P 		790.1
(1.1)
P 		978.5
(3)
P 		591
avr 787.1
(1) 		756
(0.7) 		391.1
(-3) 		423.8
(-2.7) 		807.9
(1.2) 		980.4
(3) 		589
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173305_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27625
Sequence Type Exemplar sequence
Representative Public ID CEK053C5R NCBI
Target Description CEK053C5R_rc /REP_DB=TREMBL Accession /GB=D64491 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk53c5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028037(11), NM_001028036(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028037 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
NM_001028036 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
F49E11.1c ENSEMBL cdna:known chromosome:CEL140:IV:13009105:13035916:1 gene:F49E11.1 11/11 A 
F49E11.1b ENSEMBL cdna:known chromosome:CEL140:IV:13009122:13035916:1 gene:F49E11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178250 3 2 NM_001028036 173305_s_at (11), 175032_at (11)
NM_001028037 173305_s_at (11), 175032_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13035604-13035878(+) 80.77 80.77
Public Domain and Genome References
Ensembl F49E11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03528 Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAB03351.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAA94353.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] ref|NP_001023207.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAB54274 Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54308.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54254.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] ref|NP_001023208.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] gb|AAM09088.1| minibrain kinase 2 [Caenorhabditis elegans] 0.0
blast CAA94352 Hypothetical protein F49E11.1a [Caenorhabditis elegans] ref|NP_502492.2| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAE56332 Hypothetical protein CBG23998 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-63
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-63
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 2.0E-66
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 9.2E-68
Sequence
Target Sequence 
>C. ELEGANS:173305_S_AT
tgtcattgtggtgaagctcgtcgcttaaaaagtgacgctgctgaaaccttcacgacattc
ttacccannnnnccaaatgaannnnnntcttcgttanaaacagacatgtcttttccctac
caacttgtcccttccccaattgtccccaatatatatgaaattactcttctttctagttgc
aattgattgtcttacatcttccacgcgatttttgttgtctgaaaatcgcgctggtcgcc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCATTGTGGTGAAGCTCGTCGCT 347 559 56 Antisense
AGCTCGTCGCTTAAAAAGTGACGCT 501 59 70 Antisense
AAAAGTGACGCTGCTGAAACCTTCA 340 133 83 Antisense
TGAAACCTTCACGACATTCTTACCC 139 573 97 Antisense
AAACAGACATGTCTTTTCCCTACCA 206 111 153 Antisense
CTTCCCCAATTGTCCCCAATATATA 167 219 186 Antisense
AAATTACTCTTCTTTCTAGTTGCAA 154 115 213 Antisense
TTCTTTCTAGTTGCAATTGATTGTC 483 689 222 Antisense
ATTGATTGTCTTACATCTTCCACGC 283 5 237 Antisense
TCTTCCACGCGATTTTTGTTGTCTG 96 619 252 Antisense
TTGTCTGAAAATCGCGCTGGTCGCC 36 709 270 Antisense
Sequence Source Affymetrix Proprietary Database