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[173304_at]

Description CEK010E4R_rc /REP_DB=TREMBL Accession /GB=D32316 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk10e4 : 3prime end, single read.
Cluster 6grp_total > 27      4grp_total > 44      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 92
(0)
A 		101.9
(3)
A 		81.8
(-3)
A 		87.7
(-1.2)
A 		20
B 101.1
(3)
A 		92.9
(2.4)
A 		76.1
(1.1)
A 		20.6
(-3)
A 		81
C 52.1
(-2.6)
A 		95.1
(3)
A 		49.4
(-3)
A 		73.4
(0.2)
A 		46
avr 81.7
(0.5) 		96.6
(3) 		69.1
(-1.6) 		60.6
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 13.1
(-2.6)
A 		76.5
(2.8)
A 		54.2
(0.9)
A 		8.7
(-3)
A 		79.1
(3)
A 		61.1
(1.5)
A 		70
B 162.9
(3)
M 		112.2
(0.8)
A 		87.3
(-0.3)
A 		24.5
(-3)
A 		70.5
(-1)
A 		81.3
(-0.5)
A 		138
C 108
(3)
A 		86.9
(1.7)
A 		68.9
(0.6)
A 		9.1
(-3)
A 		47.7
(-0.7)
A 		87
(1.7)
A 		99
avr 94.7
(3) 		91.9
(2.8) 		70.1
(1.2) 		14.1
(-3) 		65.8
(0.8) 		76.5
(1.6) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 33.5
(3)
A 		22.1
(-0.3)
A 		21.4
(-0.5)
A 		12.8
(-3)
A 		21
B 32.2
(3)
A 		22.9
(1)
A 		17.6
(-0.2)
A 		4.4
(-3)
A 		28
C 17.3
(-1)
A 		27.3
(3)
A 		17.2
(-1.1)
A 		12.4
(-3)
A 		15
avr 27.7
(3) 		24.1
(1.8) 		18.7
(-0) 		9.9
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 2.4
(-3)
A 		27.2
(3)
A 		11.8
(-0.7)
A 		2.2
(-3)
A 		23.9
(2.2)
A 		14.9
(0)
A 		25
B 56.7
(3)
M 		37.4
(0.7)
A 		21.2
(-1.2)
A 		6.5
(-3)
A 		28.7
(-0.3)
A 		30.3
(-0.2)
A 		50
C 40
(3)
A 		26.5
(0.9)
A 		17.5
(-0.5)
A 		1.8
(-3)
A 		15.4
(-0.9)
A 		29.1
(1.3)
A 		38
avr 33
(3) 		30.4
(2.5) 		16.8
(-0.3) 		3.5
(-3) 		22.7
(0.9) 		24.8
(1.3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173304_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28037
Sequence Type Exemplar sequence
Representative Public ID CEK010E4R NCBI
Target Description CEK010E4R_rc /REP_DB=TREMBL Accession /GB=D32316 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk10e4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064560
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064560 NCBI Caenorhabditis elegans Y48B6A.6a (Y48B6A.6) mRNA, complete cds. A 
Y48B6A.6a.2 ENSEMBL cdna:known chromosome:CEL140:II:14180150:14189759:1 gene:Y48B6A.6 None
SNAP00000022961 ENSEMBL cdna:SNAP chromosome:CEL140:II:14187773:14189541:1 None
SNAP00000022962 ENSEMBL cdna:SNAP chromosome:CEL140:II:14189647:14195507:1 None
Y48B6A.6a.1 ENSEMBL cdna:known chromosome:CEL140:II:14178705:14189759:1 gene:Y48B6A.6 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14189419-14189745(+) 66.52 71.69
Public Domain and Genome References
Ensembl Y48B6A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54442 Hypothetical protein Y48B6A.6a [Caenorhabditis elegans] ref|NP_496961.2| Y48B6A.6a [Caenorhabditis elegans] 0.0
blast CAD59175 Hypothetical protein Y48B6A.6b [Caenorhabditis elegans] ref|NP_871966.1| Y48B6A.6b [Caenorhabditis elegans] 1.0E-169
blast CAB54442 Hypothetical protein Y48B6A.6a [Caenorhabditis elegans] ref|NP_496961.2| Y48B6A.6a [Caenorhabditis elegans] 2.0E-69
blast AAH73325 MGC80733 protein [Xenopus laevis] 8.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.2E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.0E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.2E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000022962 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:173304_AT
gctcgggaagtgtccaagacgctcgcaagaagttgttgatgtctcccaaggaaggtgtan
ttcggggcaaccaaacnnnntccaaccaaaagaatgaaggaccaaccgaaactgtctcga
ttcgaggaagaaatgnnnnnnnnnnnnnnnnnnnnnnnnngacaggaagnggggaaacgt
gcccgtcggganaaattcctagccagcaaatccatcttccattgatcaaaatcttgttga
actctattaattnnnnnnnnnnntcacnatatcagcccagcataatccatgaaaactcac
cagtttaactttcacgagatgccccagccccatgccccatcnnnnnccgtatttattttc
gtcatgtacaa

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGGGAAGTGTCCAAGACGCTCG 202 299 14 Antisense
AGTGTCCAAGACGCTCGCAAGAAGT 275 57 22 Antisense
GACGCTCGCAAGAAGTTGTTGATGT 381 381 31 Antisense
AGAAGTTGTTGATGTCTCCCAAGGA 632 73 41 Antisense
GAAGGACCAACCGAAACTGTCTCGA 62 335 109 Antisense
CATCTTCCATTGATCAAAATCTTGT 324 213 226 Antisense
ATATCAGCCCAGCATAATCCATGAA 464 19 282 Antisense
GCATAATCCATGAAAACTCACCAGT 337 309 293 Antisense
AACTCACCAGTTTAACTTTCACGAG 216 139 307 Antisense
AGTTTAACTTTCACGAGATGCCCCA 10 5 315 Antisense
CCGTATTTATTTTCGTCATGTACAA 605 263 360 Antisense
Sequence Source Affymetrix Proprietary Database