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[173300_s_at] Weak similarity with NADH-ubiquinone oxidoreductase subunit (Swiss Prot accession number Q05752)

Description CEK084H1R_rc /REP_DB=TREMBL Accession /GB=D71979 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk84h1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 26      6grp_total > 41      4grp_total > 25      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3262
1 2 3 4 range
A 4558.7
(-3)
P 		5853.2
(-0.1)
P 		6576.1
(1.5)
P 		7239.2
(3)
P 		2681
B 4658.8
(-3)
P 		7135.2
(1.7)
P 		6454.3
(0.4)
P 		7820.7
(3)
P 		3162
C 4660.9
(-3)
P 		6627.4
(2.9)
P 		5270.9
(-1.2)
P 		6646.6
(3)
P 		1986
avr 4626.1
(-3) 		6538.6
(1.4) 		6100.4
(0.4) 		7235.5
(3) 		2609

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3859
1 2 3 4 5 6 range
A 6388.8
(2.8)
P 		4904.3
(-1.6)
P 		6448.7
(3)
P 		5071.8
(-1.1)
P 		5511.4
(0.2)
P 		4433.3
(-3)
P 		2015
B 4832.9
(-2.4)
P 		5601.6
(-1)
P 		7838.1
(3)
P 		6589.3
(0.8)
P 		5415.1
(-1.4)
P 		4498.4
(-3)
P 		3340
C 4969.7
(-0.7)
P 		5976.2
(1.7)
P 		5634.1
(0.9)
P 		6527
(3)
P 		5305.8
(0.1)
P 		3979.6
(-3)
P 		2547
avr 5397.1
(-0.2) 		5494
(0.1) 		6640.3
(3) 		6062.7
(1.5) 		5410.8
(-0.2) 		4303.8
(-3) 		2337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 846
1 2 3 4 range
A 1659.8
(2.5)
P 		1270.6
(-1.1)
P 		1719.1
(3)
P 		1059.7
(-3)
P 		659
B 1485.7
(-3)
P 		1762.2
(3)
P 		1495.6
(-2.8)
P 		1666.6
(0.9)
P 		277
C 1542.9
(0.2)
P 		1905.4
(3)
P 		1837.1
(2.5)
P 		1120.7
(-3)
P 		785
avr 1562.8
(1.2) 		1646.1
(2.4) 		1683.9
(3) 		1282.3
(-3) 		402

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1121
1 2 3 4 5 6 range
A 1177.7
(-2.1)
P 		1744.2
(3)
P 		1404.4
(-0.1)
P 		1286.3
(-1.1)
P 		1663.1
(2.3)
P 		1079.4
(-3)
P 		665
B 1681.6
(-3)
P 		1864.5
(-0.9)
P 		1903.3
(-0.4)
P 		1745.3
(-2.2)
P 		2200.1
(3)
P 		1677.4
(-3)
P 		523
C 1842.1
(3)
P 		1823.5
(2.8)
P 		1434
(-1.6)
P 		1312.6
(-3)
P 		1716.9
(1.6)
P 		1332.2
(-2.8)
P 		530
avr 1567.1
(-0.5) 		1810.7
(2.4) 		1580.6
(-0.4) 		1448.1
(-2) 		1860
(3) 		1363
(-3) 		497
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173300_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27731
Sequence Type Exemplar sequence
Representative Public ID CEK084H1R NCBI
Target Description CEK084H1R_rc /REP_DB=TREMBL Accession /GB=D71979 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk84h1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064438(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064438 NCBI Caenorhabditis elegans F45H10.3 (F45H10.3) mRNA, complete cds. 11/11 A 
SNAP00000000153 ENSEMBL cdna:SNAP chromosome:CEL140:II:13493147:13494489:-1 11/11 None
GENEFINDER00000000159 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13493147:13494489:-1 10/11 None
F45H10.3.2 ENSEMBL cdna:known chromosome:CEL140:II:13493077:13494496:-1 gene:F45H10.3 11/11 None
F45H10.3.3 ENSEMBL cdna:known chromosome:CEL140:II:13493077:13494498:-1 gene:F45H10.3 11/11 None
F45H10.3.1 ENSEMBL cdna:known chromosome:CEL140:II:13493077:13494498:-1 gene:F45H10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13493088-13493389(-) 80.0 80.0
Public Domain and Genome References
Gene Title Weak similarity with NADH-ubiquinone oxidoreductase subunit (Swiss Prot accession number Q05752)
Ensembl F45H10.3
Entrez Gene 174993 Entrez gene
SwissProt O02267 EMBL-EBI
WormBase CE10546 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04385 Hypothetical protein F45H10.3 [Caenorhabditis elegans] ref|NP_496839.1| F45H10.3 [Caenorhabditis elegans] 3.0E-95
blast CAB04385 Hypothetical protein F45H10.3 [Caenorhabditis elegans] ref|NP_496839.1| F45H10.3 [Caenorhabditis elegans] 8.0E-88
blast CAE73341 Hypothetical protein CBG20772 [Caenorhabditis briggsae] 3.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR009947 EMBL-EBI NADH:ubiquinone oxidoreductase subunit B14.5a 2.5E-7
Sequence
Target Sequence 
>C. ELEGANS:173300_S_AT
aggaggtgttcatcacaagctcgctgataattattacttgacaagagacggccgccgaaa
cgtcgatgctccgaaagtgctctactcgtctggcgagtttgttggaggagnnnnngcaca
gccagcgcaagtgaatcaaggagtcgccacaaacttcggattgacagctccaacccctgg
ttttggagccgaatggaaccgatccaaccagaatgagctggccagccancagaagaacac
cgaattcgagtatttgcagcgtttcgacaagttcac

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAGGTGTTCATCACAAGCTCGCT 167 57 30 Antisense
AGAGACGGCCGCCGAAACGTCGATG 140 69 73 Antisense
AACGTCGATGCTCCGAAAGTGCTCT 674 143 88 Antisense
AAAGTGCTCTACTCGTCTGGCGAGT 497 121 103 Antisense
TCGTCTGGCGAGTTTGTTGGAGGAG 428 605 115 Antisense
GCACAGCCAGCGCAAGTGAATCAAG 56 317 145 Antisense
CAAGGAGTCGCCACAAACTTCGGAT 291 181 166 Antisense
AACTTCGGATTGACAGCTCCAACCC 133 139 181 Antisense
ACAGCTCCAACCCCTGGTTTTGGAG 224 109 193 Antisense
ACACCGAATTCGAGTATTTGCAGCG 190 105 266 Antisense
ATTTGCAGCGTTTCGACAAGTTCAC 506 17 281 Antisense
Sequence Source Affymetrix Proprietary Database