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[173297_at]

Description CEK081G4R_rc /REP_DB=TREMBL Accession /GB=D71803 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk81g4 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 85      4grp_high_8_8 > 06      6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4861
1 2 3 4 range
A 261.7
(-3)
P 		231.7
(-3)
P 		288.3
(-2.9)
P 		4707.3
(3)
P 		4476
B 289.2
(-2.9)
P 		262.3
(-2.9)
A 		214.5
(-3)
P 		3852.1
(3)
P 		3638
C 261.8
(-3)
P 		281.3
(-3)
P 		271.5
(-3)
P 		5075.6
(3)
P 		4814
avr 270.9
(-3) 		258.4
(-3) 		258.1
(-3) 		4545
(3) 		4287

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 218.7
(0.5)
P 		213.4
(-0.1)
P 		239.5
(3)
P 		224
(1.1)
P 		223.5
(1.1)
P 		189.5
(-3)
P 		50
B 202.2
(-3)
P 		248.3
(-1.1)
P 		319.1
(1.9)
P 		203.3
(-3)
P 		346.2
(3)
P 		339.3
(2.7)
P 		144
C 215.3
(-2.7)
P 		245.8
(-0.6)
P 		211.6
(-3)
A 		238.6
(-1.1)
P 		293.4
(2.7)
P 		297.7
(3)
P 		86
avr 212.1
(-3) 		235.8
(-1.1) 		256.7
(0.5) 		222
(-2.2) 		287.7
(3) 		275.5
(2) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 806
1 2 3 4 range
A 95.3
(-2.6)
P 		50.3
(-3)
P 		75.4
(-2.8)
P 		689.1
(3)
P 		639
B 92.2
(-2.7)
P 		64.8
(-2.9)
A 		49.7
(-3)
P 		820.9
(3)
P 		771
C 86.7
(-3)
P 		80.9
(-3)
P 		94.6
(-2.9)
P 		855.8
(3)
P 		775
avr 91.4
(-2.8) 		65.3
(-3) 		73.2
(-2.9) 		788.6
(3) 		723

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 40.3
(-3)
P 		75.9
(3)
P 		52.2
(-1)
P 		56.8
(-0.2)
P 		67.4
(1.6)
P 		46.1
(-2)
P 		36
B 70.3
(-1.9)
P 		82.6
(-1)
P 		77.5
(-1.4)
P 		53.8
(-3)
P 		140.7
(3)
P 		126.5
(2)
P 		87
C 79.8
(0.7)
P 		75
(0.1)
P 		53.9
(-2.3)
A 		48
(-3)
P 		94.9
(2.4)
P 		99.7
(3)
P 		52
avr 63.5
(-1.7) 		77.8
(0.1) 		61.2
(-2) 		52.9
(-3) 		101
(3) 		90.8
(1.7) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173297_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27971
Sequence Type Exemplar sequence
Representative Public ID CEK081G4R NCBI
Target Description CEK081G4R_rc /REP_DB=TREMBL Accession /GB=D71803 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk81g4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058435
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058435 NCBI Caenorhabditis elegans Y48G8AL.13 (Y48G8AL.13) mRNA, complete cds. None
Y48G8AL.13.1 ENSEMBL cdna:known chromosome:CEL140:I:1223965:1226813:-1 gene:Y48G8AL.13 None
SNAP00000040535 ENSEMBL cdna:SNAP chromosome:CEL140:I:1222649:1226799:-1 None
GENEFINDER00000040548 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1224163:1226799:-1 None
Y48G8AL.13.3 ENSEMBL cdna:known chromosome:CEL140:I:1224007:1226815:-1 gene:Y48G8AL.13 None
Y48G8AL.13.2 ENSEMBL cdna:known chromosome:CEL140:I:1224007:1229283:-1 gene:Y48G8AL.13 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1223964-1224327(-) 96.97 96.97
Public Domain and Genome References
Gene Title
Ensembl Y48G8AL.13
Entrez Gene 171699 Entrez gene
SwissProt Q9BL09 EMBL-EBI
WormBase CE22187 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29899 Hypothetical protein Y48G8AL.13 [Caenorhabditis elegans] ref|NP_490836.1| Y48G8AL.13 [Caenorhabditis elegans] 1.0E-142
blast CAE63917 Hypothetical protein CBG08489 [Caenorhabditis briggsae] 1.0E-126
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490836.1 5 39-61,81-100,110-132,168-190,200-217
Y48G8AL.13.1 5 39-61,81-100,110-132,168-190,200-217
Y48G8AL.13.3 5 39-61,81-100,110-132,168-190,200-217
Y48G8AL.13.2 5 39-61,81-100,110-132,168-190,200-217
SNAP00000040535 5 39-61,81-100,110-132,168-190,200-217
GENEFINDER00000040548 5 39-61,81-100,110-132,168-190,200-217
Sequence
Target Sequence 
>C. ELEGANS:173297_AT
aacgacaggctctcgacgaggacagctgtgacccgttgggatctttgaagaaggatcagg
aagtggagcagaagcaccagcagaagtcggagcgatcggagcgatcgacgaagacgacgg
cgaagaaaatcgagtgaaaattttgaataacgaatcaacgaacaacaccagatcntcttc
gtcatnnnnncatgtcttcgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nntaggtccgagccttaatttaatacttgtgtgttcagagaattgtgtgtgtgtg

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGACAGGCTCTCGACGAGGACAG 307 121 41 Antisense
GGCTCTCGACGAGGACAGCTGTGAC 233 539 48 Antisense
ACAGCTGTGACCCGTTGGGATCTTT 320 107 62 Antisense
TGACCCGTTGGGATCTTTGAAGAAG 373 545 69 Antisense
GGAAGTGGAGCAGAAGCACCAGCAG 29 533 99 Antisense
AGCACCAGCAGAAGTCGGAGCGATC 395 77 113 Antisense
AGAAGTCGGAGCGATCGGAGCGATC 390 73 122 Antisense
GAGCGATCGACGAAGACGACGGCGA 199 387 139 Antisense
TAACGAATCAACGAACAACACCAGA 76 639 188 Antisense
GGTCCGAGCCTTAATTTAATACTTG 355 285 285 Antisense
GTGTTCAGAGAATTGTGTGTGTGTG 547 485 311 Antisense
Sequence Source Affymetrix Proprietary Database