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[173294_at] carbonic anhydrase

Description CEK072DXR_rc /REP_DB=TREMBL Accession /GB=D66017 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk72d10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 29      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 40      6grp_total > 29      4grp_total > 54      6grp_cgc > 03      6grp_high1_sub > Cu      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1169
1 2 3 4 range
A 1242.7
(3)
P 		1041.8
(1.6)
P 		947.7
(0.9)
P 		406.6
(-3)
P 		836
B 1460.1
(3)
P 		775.3
(-2.5)
P 		886.2
(-1.6)
P 		710.4
(-3)
P 		750
C 1575.4
(3)
P 		766.4
(-1.3)
P 		700.2
(-1.6)
P 		442.1
(-3)
P 		1133
avr 1426.1
(3) 		861.2
(-0.7) 		844.7
(-0.8) 		519.7
(-3) 		906

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 986
1 2 3 4 5 6 range
A 624.9
(2.4)
P 		556.2
(1.3)
P 		290.4
(-3)
P 		486.3
(0.2)
P 		662.2
(3)
P 		594
(1.9)
P 		372
B 1168.3
(3)
P 		798.6
(0.6)
P 		427.6
(-1.8)
P 		251.9
(-3)
P 		666.7
(-0.3)
P 		387.8
(-2.1)
P 		916
C 1231.9
(3)
P 		833.1
(0.6)
P 		321.7
(-2.5)
P 		246.2
(-3)
P 		767.8
(0.2)
P 		517.8
(-1.3)
P 		986
avr 1008.4
(3) 		729.3
(0.5) 		346.6
(-2.8) 		328.1
(-3) 		698.9
(0.3) 		499.9
(-1.5) 		680
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 462
1 2 3 4 range
A 452.5
(3)
P 		226.1
(-0.5)
P 		247.8
(-0.1)
P 		59.5
(-3)
P 		393
B 465.6
(3)
P 		191.5
(-2.2)
P 		205.3
(-2)
P 		151.4
(-3)
P 		314
C 521.5
(3)
P 		220.3
(-1)
P 		244
(-0.7)
P 		74.5
(-3)
P 		447
avr 479.9
(3) 		212.6
(-1.2) 		232.4
(-0.9) 		95.1
(-3) 		385

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 115.2
(-0.7)
P 		197.8
(2.9)
P 		63.2
(-3)
P 		123.3
(-0.4)
P 		199.8
(3)
P 		144.6
(0.6)
P 		137
B 406.5
(3)
P 		265.8
(0.5)
P 		103.8
(-2.3)
P 		66.7
(-3)
P 		270.9
(0.6)
P 		144.6
(-1.6)
P 		340
C 456.6
(3)
P 		254.2
(0)
P 		81.9
(-2.5)
P 		49.5
(-3)
P 		248.4
(-0.1)
P 		173.3
(-1.2)
P 		407
avr 326.1
(3) 		239.3
(0.9) 		83
(-2.9) 		79.8
(-3) 		239.7
(0.9) 		154.2
(-1.2) 		246
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27691
Sequence Type Exemplar sequence
Representative Public ID CEK072DXR NCBI
Target Description CEK072DXR_rc /REP_DB=TREMBL Accession /GB=D66017 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk72d10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078273
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078273 NCBI Caenorhabditis elegans Carbonic AnHydrase family member (cah-3) (cah-3) mRNA, complete cds. A 
GENEFINDER00000038240 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16486921:16497427:1 None
K05G3.3 ENSEMBL cdna:known chromosome:CEL140:X:16487427:16489298:1 gene:K05G3.3 A 
SNAP00000038236 ENSEMBL cdna:SNAP chromosome:CEL140:X:16487447:16489024:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16488926-16489298(+) 94.34 94.34
Public Domain and Genome References
Gene Title carbonic anhydrase
Gene Symbol cah-3
Ensembl K05G3.3
Entrez Gene 181713 Entrez gene
SwissProt Q27504 EMBL-EBI
WormBase CE04755 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586208_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFA.18716.1.S1_S_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFA.2644.1.A1_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFAAFFX.13723.1.S1_S_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
DROSOPHILA_2:1636374_AT Carbonic anhydrase 1 dm
DROSGENOME1:152261_AT Carbonic anhydrase 1 dm
CHICKEN:GGA.3986.2.S1_A_AT carbonic anhydrase II gga
HG-U133A:209301_AT carbonic anhydrase II hs
HG-U133_PLUS_2:209301_AT carbonic anhydrase II hs
HG-FOCUS:209301_AT carbonic anhydrase II hs
HG-U133A_2:209301_AT carbonic anhydrase II hs
HUGENEFL:Y00339_S_AT carbonic anhydrase II hs
U133_X3P:G179794_3P_AT carbonic anhydrase II hs
HG-U95AV2:40095_AT carbonic anhydrase II hs
HG-U95D:75200_R_AT carbonic anhydrase II hs
MOE430A:1448752_AT carbonic anhydrase 2 mm
MOUSE430A_2:1448752_AT carbonic anhydrase 2 mm
MOUSE430_2:1448752_AT carbonic anhydrase 2 mm
MU11KSUBA:K00811_S_AT carbonic anhydrase 2 mm
MG-U74AV2:92642_AT carbonic anhydrase 2 mm
MU19KSUBB:TC26629_AT Carbonic anhydrase 2 (Car2), mRNA mm
MU19KSUBB:TC26630_S_AT Carbonic anhydrase 2 (Car2), mRNA mm
RG-U34A:X58294_AT carbonic anhydrase 2 rn
RG-U34A:U60578CDS_S_AT carbonic anhydrase 2 rn
RAE230A:1386922_AT carbonic anhydrase 2 rn
RAT230_2:1386922_AT carbonic anhydrase 2 rn
RAE230A:1367733_AT carbonic anhydrase 2 rn
RAT230_2:1367733_AT carbonic anhydrase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6730 one-carbon compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4089 carbonate dehydratase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52498 Carbonic anhydrase protein 3 [Caenorhabditis elegans] ref|NP_510674.1| Carbonic AnHydrase family member (cah-3) [Caenorhabditis elegans] sp|Q27504|CAH3_CAEEL Putative carbonic anhydrase 3 (Carbonate dehydratase 3) 1.0E-147
blast AAB52498 Carbonic anhydrase protein 3 [Caenorhabditis elegans] ref|NP_510674.1| Carbonic AnHydrase family member (cah-3) [Caenorhabditis elegans] sp|Q27504|CAH3_CAEEL Putative carbonic anhydrase 3 (Carbonate dehydratase 3) 1.0E-145
blast CAE69965 Hypothetical protein CBG16360 [Caenorhabditis briggsae] 1.0E-144
blast CAE69965 Hypothetical protein CBG16360 [Caenorhabditis briggsae] 1.0E-141
blast AAB52497 Hypothetical protein K05G3.2 [Caenorhabditis elegans] ref|NP_510675.2| K05G3.2 [Caenorhabditis elegans] 5.0E-67
blast AAA80437 Carbonic anhydrase protein 5 [Caenorhabditis elegans] ref|NP_509186.3| Carbonic AnHydrase family member (cah-5) [Caenorhabditis elegans] sp|Q10462|CAH5_CAEEL Putative carbonic anhydrase 5 precursor (Carbonate dehydratase 5) 2.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 2.7E-55
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 1.6E-57
Sequence
Target Sequence 
>C. ELEGANS:173294_AT
gttcacattgtagcttattaggattttcccagnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnngagacacaaatgccanncacaaacttcatggtcccaagcaacttttgtcaca
aatccccgttttcctctgatttcaacacttcaagccagcccgatgtctannnnncttatg
tagtaattcgcaatttctgccaaatcattgcaatattctagttactctgacacgaaaccc
atgggttcccact

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCACATTGTAGCTTATTAGGATT 366 445 89 Antisense
TTGTAGCTTATTAGGATTTTCCCAG 377 705 96 Antisense
CACAAACTTCATGGTCCCAAGCAAC 587 193 174 Antisense
ATGGTCCCAAGCAACTTTTGTCACA 457 51 184 Antisense
CCAAGCAACTTTTGTCACAAATCCC 162 275 190 Antisense
CGTTTTCCTCTGATTTCAACACTTC 324 245 215 Antisense
TGATTTCAACACTTCAAGCCAGCCC 206 565 225 Antisense
ATTTCTGCCAAATCATTGCAATATT 368 17 281 Antisense
ATTGCAATATTCTAGTTACTCTGAC 561 5 295 Antisense
GTTACTCTGACACGAAACCCATGGG 478 441 309 Antisense
GACACGAAACCCATGGGTTCCCACT 92 285 317 Antisense
Sequence Source Affymetrix Proprietary Database