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[173288_at]

Description CEC5394_rc /REP_DB=TREMBL Accession /GB=C11539 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk182d5 : 3prime end, single read.
Cluster 6grp_total > 90      4grp_total > 56      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 4.5
(-3)
A 		7.1
(3)
A 		4.7
(-2.5)
A 		4.8
(-2.3)
A 		3
B 2
(-2.8)
A 		5.7
(-1.5)
A 		18.2
(3)
A 		1.5
(-3)
A 		17
C 4
(-1.7)
A 		7.9
(3)
A 		3.7
(-2)
A 		2.9
(-3)
A 		5
avr 3.5
(-2.6) 		6.9
(0.9) 		8.9
(3) 		3.1
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 15.8
(3)
A 		3.4
(-2.1)
A 		5.2
(-1.4)
A 		4.7
(-1.6)
A 		10.9
(1)
A 		1.3
(-3)
A 		15
B 5.9
(-1.5)
A 		5.8
(-1.5)
A 		3.2
(-2.4)
A 		18.6
(3)
A 		1.5
(-3)
A 		3.3
(-2.4)
A 		17
C 2
(-3)
A 		4.4
(0.2)
A 		3.3
(-1.3)
A 		6.5
(3)
A 		5.1
(1.1)
A 		2.9
(-1.8)
A 		5
avr 7.9
(1.4) 		4.5
(-1.4) 		3.9
(-1.9) 		9.9
(3) 		5.8
(-0.3) 		2.5
(-3) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1.6
(3)
A 		1.5
(2.3)
A 		1.2
(0.3)
A 		0.7
(-3)
A 		1
B 0.6
(-2.5)
A 		1.4
(-1.3)
A 		4.2
(3)
A 		0.3
(-3)
A 		4
C 1.3
(-0.3)
A 		2.3
(3)
A 		1.3
(-0.3)
A 		0.5
(-3)
A 		2
avr 1.2
(-0.5) 		1.7
(1.2) 		2.2
(3) 		0.5
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 2.9
(2.2)
A 		1.2
(-1.2)
A 		1.1
(-1.4)
A 		1.2
(-1.2)
A 		3.3
(3)
A 		0.3
(-3)
A 		3
B 2
(-1)
A 		1.9
(-1.2)
A 		0.8
(-2.7)
A 		4.9
(3)
A 		0.6
(-3)
A 		1.2
(-2.2)
A 		4
C 0.8
(-3)
A 		1.3
(0.8)
A 		0.8
(-3)
A 		1.3
(0.8)
A 		1.6
(3)
A 		1
(-1.5)
A 		1
avr 1.9
(0.9) 		1.5
(-0.5) 		0.9
(-2.6) 		2.5
(3) 		1.8
(0.5) 		0.8
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173288_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27803
Sequence Type Exemplar sequence
Representative Public ID CEC5394 NCBI
Target Description CEC5394_rc /REP_DB=TREMBL Accession /GB=C11539 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk182d5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059138
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
F56A3.4 ENSEMBL cdna:known chromosome:CEL140:I:5171286:5175525:-1 gene:F56A3.4 A 
GENEFINDER00000016717 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5171146:5175524:-1 None
SNAP00000016706 ENSEMBL cdna:SNAP chromosome:CEL140:I:5171497:5173897:-1 None
NM_059138 NCBI Caenorhabditis elegans SPindle Defective family member (spd-5) (spd-5) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5171393-5171836(-) 91.6 91.6
Public Domain and Genome References
Gene Title
Gene Symbol spd-5
Ensembl F56A3.4
Entrez Gene 172155 Entrez gene
SwissProt P91349 EMBL-EBI
WormBase CE28834 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37802 Spindle defective protein 5 [Caenorhabditis elegans] ref|NP_491539.1| SPindle Defective family member (spd-5) [Caenorhabditis elegans] sp|P91349|SPD5_CAEEL Spindle-defective protein 0.0
blast CAE56131 Hypothetical protein CBG23743 [Caenorhabditis briggsae] 0.0
blast XP_473881 OSJNBa0008M17.5 [Oryza sativa (japonica cultivar-group)] emb|CAE04334.2| OSJNBa0008M17.5 [Oryza sativa (japonica cultivar-group)] 4.0E-30
Sequence
Target Sequence 
>C. ELEGANS:173288_AT
cttgatgcttgaggaccaaatctagcagtcccagaaggacctgtaactgccgaaatccgc
catcccgacacttggtgtcggaattccaagacagactcgccaccgacattgccacaatcg
cagctgatatgggacgacctgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGATGCTTGAGGACCAAATCTAG 172 217 139 Antisense
GAGGACCAAATCTAGCAGTCCCAGA 176 405 149 Antisense
ACCAAATCTAGCAGTCCCAGAAGGA 469 83 153 Antisense
GTCCCAGAAGGACCTGTAACTGCCG 501 475 166 Antisense
AGAAGGACCTGTAACTGCCGAAATC 375 73 171 Antisense
AAGGACCTGTAACTGCCGAAATCCG 326 161 173 Antisense
CGACACTTGGTGTCGGAATTCCAAG 287 251 204 Antisense
TCGGAATTCCAAGACAGACTCGCCA 582 605 216 Antisense
GACATTGCCACAATCGCAGCTGATA 630 369 243 Antisense
GCCACAATCGCAGCTGATATGGGAC 283 279 249 Antisense
CGCAGCTGATATGGGACGACCTGTG 35 255 257 Antisense
Sequence Source Affymetrix Proprietary Database