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[173282_s_at] trehalase

Description C53291_rc /REP_DB=TREMBL Accession /GB=C53291 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk296e1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 32      6grp_total > 31      4grp_total > 55      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 822
1 2 3 4 range
A 1249
(3)
P 		801
(-0.5)
P 		787.2
(-0.6)
P 		485.2
(-3)
P 		764
B 1307.1
(3)
P 		697.8
(-2.2)
P 		851.3
(-0.9)
P 		602.4
(-3)
P 		705
C 1302.4
(3)
P 		723.7
(-1.6)
P 		908.8
(-0.1)
P 		550.7
(-3)
P 		752
avr 1286.2
(3) 		740.8
(-1.4) 		849.1
(-0.5) 		546.1
(-3) 		740

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 761
1 2 3 4 5 6 range
A 1189.8
(0.5)
P 		1207.1
(0.6)
P 		1511.2
(3)
P 		1192.5
(0.5)
P 		1063.8
(-0.5)
P 		750.2
(-3)
P 		761
B 1340.7
(3)
P 		1293.5
(2.4)
P 		1315.4
(2.7)
P 		1248.8
(1.8)
P 		1043.9
(-0.9)
P 		889.8
(-3)
P 		451
C 1061.8
(-0.1)
P 		1295
(3)
P 		1160.6
(1.2)
P 		1133.8
(0.9)
P 		838.8
(-3)
P 		966.6
(-1.3)
P 		456
avr 1197.4
(1.3) 		1265.2
(2.2) 		1329.1
(3) 		1191.7
(1.2) 		982.2
(-1.5) 		868.9
(-3) 		460
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 384
1 2 3 4 range
A 454.8
(3)
P 		173.9
(-1.4)
P 		205.8
(-0.9)
P 		71
(-3)
P 		384
B 416.8
(3)
P 		172.3
(-2.1)
P 		197.3
(-1.6)
P 		128.4
(-3)
P 		288
C 431.1
(3)
P 		208.1
(-1)
P 		316.7
(1)
P 		92.8
(-3)
P 		338
avr 434.2
(3) 		184.8
(-1.4) 		239.9
(-0.5) 		97.4
(-3) 		337

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 219.3
(-2.1)
P 		429.3
(3)
P 		329.1
(0.6)
P 		302.4
(-0.1)
P 		321
(0.4)
P 		182.6
(-3)
P 		247
B 466.5
(3)
P 		430.6
(1.5)
P 		319.4
(-3)
P 		330.8
(-2.5)
P 		424.1
(1.3)
P 		331.8
(-2.5)
P 		147
C 393.6
(2.9)
P 		395.2
(3)
P 		295.4
(-0.6)
P 		228
(-3)
P 		271.4
(-1.4)
P 		323.6
(0.4)
P 		167
avr 359.8
(0.5) 		418.4
(3) 		314.6
(-1.5) 		287.1
(-2.7) 		338.8
(-0.4) 		279.3
(-3) 		139
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173282_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27718
Sequence Type Exemplar sequence
Representative Public ID C53291 NCBI
Target Description C53291_rc /REP_DB=TREMBL Accession /GB=C53291 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk296e1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073558 NCBI Caenorhabditis elegans W05E10.4 (trehalase) mRNA, complete cds. 11/11 A 
GENEFINDER00000019395 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11704868:11718420:-1 11/11 None
W05E10.4 ENSEMBL cdna:known chromosome:CEL140:V:11711920:11718492:-1 gene:W05E10.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019390 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11712376-11715137(-) 97.16 99.15
Public Domain and Genome References
Gene Title trehalase
Gene Symbol tre-3
Ensembl W05E10.4
Entrez Gene 179604 Entrez gene
SwissProt Q23176 EMBL-EBI
WormBase CE06551 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5991 trehalose metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4555 alpha,alpha-trehalase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01250 Hypothetical protein W05E10.4 [Caenorhabditis elegans] ref|NP_505959.1| W05E10.4 [Caenorhabditis elegans] emb|CAD54509.1| trehalase [Caenorhabditis elegans] 0.0
blast CAE75311 Hypothetical protein CBG23281 [Caenorhabditis briggsae] 0.0
blast CAB01249 Hypothetical protein W05E10.3 [Caenorhabditis elegans] ref|NP_505958.1| C.Elegans Homeobox family member (ceh-32) [Caenorhabditis elegans] gb|AAG49583.1| CEH-32 [Caenorhabditis elegans] sp|Q23175|HM32_CAEEL Homeobox protein ceh-32 0.0
blast AAK31441 Trehalase protein 5 [Caenorhabditis elegans] ref|NP_493649.2| C23H3.7 [Caenorhabditis elegans] emb|CAD54512.1| trehalase [Caenorhabditis elegans] 1.0E-147
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 6.00000021222513E-6
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 2.59999990463257
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.0E-126
Pfam IPR001356 EMBL-EBI Homeobox 4.4E-10
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173282_S_AT
gagtgttctatgaaaccggccacatgtgggagaagtacaacgtgatcggcagctacccac
aaccaggatctggaggtgaatatgatgttcaggatggatttggatggagcaacggagcta
tattggatcttctcttgacatataacgatcgattatttgttccagaaaactttgtcaacg
ctgctgtcacaccttcggttgaatctactaccaaatctgctccaaaatatgctaagctac
catgggtactcgttttcgctttcatgattcgtcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTGTTCTATGAAACCGGCCACAT 190 401 25 Antisense
GAAACCGGCCACATGTGGGAGAAGT 565 357 36 Antisense
AAGTACAACGTGATCGGCAGCTACC 207 155 57 Antisense
GCAACGGAGCTATATTGGATCTTCT 607 319 133 Antisense
TGGATCTTCTCTTGACATATAACGA 523 549 148 Antisense
TAACGATCGATTATTTGTTCCAGAA 204 639 167 Antisense
CTGTCACACCTTCGGTTGAATCTAC 530 239 208 Antisense
AATCTACTACCAAATCTGCTCCAAA 197 169 226 Antisense
GCTAAGCTACCATGGGTACTCGTTT 50 301 255 Antisense
GTACTCGTTTTCGCTTTCATGATTC 284 459 270 Antisense
GTTTTCGCTTTCATGATTCGTCAAC 83 449 276 Antisense
Sequence Source Affymetrix Proprietary Database