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[173279_s_at] long-chain enoyl-CoA hydratase

Description CEK005G6R_rc /REP_DB=TREMBL Accession /GB=D27896 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk5g6 : 3prime end, single read.
Cluster 6grp_total > 56      4grp_total > 56      6grp_cgc > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 8.3
(-3)
A 		57.2
(0.7)
A 		87.3
(3)
A 		49.6
(0.1)
A 		79
B 66.3
(-0.2)
A 		53.9
(-1.3)
A 		100.7
(3)
A 		35.3
(-3)
A 		65
C 60.2
(-3)
M 		63.6
(-1.1)
A 		69.1
(1.9)
A 		71
(3)
P 		11
avr 44.9
(-3) 		58.2
(-1) 		85.7
(3) 		52
(-2) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 47.9
(-1)
A 		46.2
(-1.1)
A 		40.2
(-1.6)
A 		47
(-1.1)
A 		23.2
(-3)
A 		96.8
(3)
P 		74
B 74.3
(3)
A 		73.8
(2.9)
A 		56.8
(-1.2)
A 		49
(-3)
A 		50.3
(-2.7)
A 		65.2
(0.8)
A 		25
C 49.2
(1.3)
P 		47
(1.1)
A 		66
(3)
A 		52.8
(1.7)
A 		5.7
(-3)
A 		64.3
(2.8)
A 		60
avr 57.1
(0.8) 		55.7
(0.6) 		54.3
(0.4) 		49.6
(-0.2) 		26.4
(-3) 		75.4
(3) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 3
(-3)
A 		12.4
(-0.2)
A 		22.8
(3)
A 		7.3
(-1.7)
A 		20
B 21.2
(2.2)
A 		13.3
(-0.8)
A 		23.3
(3)
A 		7.5
(-3)
A 		16
C 19.9
(0.9)
M 		18.3
(0.1)
A 		24.1
(3)
A 		12
(-3)
P 		12
avr 14.7
(-0.6) 		14.7
(-0.6) 		23.4
(3) 		8.9
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 8.8
(-2.3)
A 		16.4
(0.4)
A 		8.8
(-2.3)
A 		11.9
(-1.2)
A 		7
(-3)
A 		23.6
(3)
P 		17
B 25.9
(3)
A 		24.6
(2.4)
A 		13.8
(-2.6)
A 		13
(-3)
A 		20.4
(0.4)
A 		24.3
(2.3)
A 		13
C 18.2
(2)
P 		14.4
(0.8)
A 		16.8
(1.6)
A 		10.6
(-0.3)
A 		1.8
(-3)
A 		21.5
(3)
A 		20
avr 17.6
(0.5) 		18.5
(0.9) 		13.1
(-1.5) 		11.8
(-2.1) 		9.7
(-3) 		23.1
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173279_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27846
Sequence Type Exemplar sequence
Representative Public ID CEK005G6R NCBI
Target Description CEK005G6R_rc /REP_DB=TREMBL Accession /GB=D27896 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk5g6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074409(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074409 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-1) (ech-1) mRNA, complete cds. 11/11 A 
SNAP00000013693 ENSEMBL cdna:SNAP chromosome:CEL140:V:15360378:15363324:-1 11/11 None
GENEFINDER00000013695 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15360378:15363324:-1 11/11 None
C29F3.1 ENSEMBL cdna:known chromosome:CEL140:V:15360282:15363324:-1 gene:C29F3.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15360281-15361077(-) 94.63 94.63
Public Domain and Genome References
Gene Title long-chain enoyl-CoA hydratase
Gene Symbol ech-1
Ensembl C29F3.1
Entrez Gene 180037 Entrez gene
SwissProt O17612 EMBL-EBI
WormBase CE08435 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6631 fatty acid metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02799 Hypothetical protein C29F3.1 [Caenorhabditis elegans] ref|NP_506810.1| Enoyl-CoA Hydratase family member (ech-1) [Caenorhabditis elegans] 0.0
blast NP_491790 T08B2.7b [Caenorhabditis elegans] gb|AAK21464.1| Hypothetical protein T08B2.7b [Caenorhabditis elegans] 0.0
blast NP_491789 T08B2.7a [Caenorhabditis elegans] gb|AAK21462.1| Hypothetical protein T08B2.7a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 2.9E-36
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 8.2E-6
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 6.4E-58
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 3.9E-80
Sequence
Target Sequence 
>C. ELEGANS:173279_S_AT
tctcgtctctcgatttgtcaatgaggcactttcttgtcttgaaggaggtgttattagctc
accatctggacggagatatagcctctgtatttgnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnncattccggtttgtcgatctttatggagcgggcaagttggttgcggcgatggacaggt
ttgccggagtgtacgaatctgtccaatttgagccatgccaacttctcagaaatc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCGTCTCTCGATTTGTCAATGAG 372 611 98 Antisense
GTCTCTCGATTTGTCAATGAGGCAC 318 471 102 Antisense
GACGGAGATATAGCCTCTGTATTTG 278 377 166 Antisense
CATTCCGGTTTGTCGATCTTTATGG 326 217 221 Antisense
TTTGTCGATCTTTATGGAGCGGGCA 235 663 229 Antisense
GGAGCGGGCAAGTTGGTTGCGGCGA 413 519 244 Antisense
GTTGGTTGCGGCGATGGACAGGTTT 61 435 255 Antisense
GATGGACAGGTTTGCCGGAGTGTAC 189 407 267 Antisense
GCCGGAGTGTACGAATCTGTCCAAT 489 283 280 Antisense
GAATCTGTCCAATTTGAGCCATGCC 170 329 292 Antisense
GAGCCATGCCAACTTCTCAGAAATC 260 385 307 Antisense
Sequence Source Affymetrix Proprietary Database