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[173276_s_at]

Description CEC3601_rc /REP_DB=TREMBL Accession /GB=C08360 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk187b1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 18      4grp_high_8_8 > 63      6grp_highest_nonorm > 26      6grp_total > 62      4grp_total > 56      6grp_highest_sub > 03_04      6grp_cgc > 84      6grp_high1_sub > 03      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1454
1 2 3 4 range
A 4675.5
(-0.8)
P 		5267
(1.8)
P 		5543.4
(3)
P 		4186.2
(-3)
P 		1357
B 4398.1
(-2.5)
P 		4337.9
(-3)
P 		5013
(3)
P 		4344.4
(-2.9)
P 		675
C 4324.7
(-1.8)
P 		4741.1
(0.4)
P 		5256.4
(3)
P 		4089.2
(-3)
P 		1167
avr 4466.1
(-1.5) 		4782
(0.2) 		5270.9
(3) 		4206.6
(-3) 		1064

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6090
1 2 3 4 5 6 range
A 5050.8
(-0.3)
P 		4606
(-1)
P 		7170.2
(3)
P 		6880.6
(2.6)
P 		5468.5
(0.4)
P 		3280.2
(-3)
P 		3890
B 5249.2
(-2.1)
P 		5704.9
(-1.5)
P 		9370.2
(3)
P 		6909.4
(-0)
P 		6262.1
(-0.8)
P 		4518.9
(-3)
P 		4851
C 4484.6
(-3)
P 		5854
(-0.4)
P 		6582.7
(1)
P 		7620.1
(3)
P 		5625.9
(-0.8)
P 		4475.3
(-3)
P 		3145
avr 4928.2
(-1.6) 		5388.3
(-0.8) 		7707.7
(3) 		7136.7
(2.1) 		5785.5
(-0.2) 		4091.5
(-3) 		3616
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1219
1 2 3 4 range
A 1702.4
(3)
P 		1143.3
(-0.1)
P 		1449.1
(1.6)
P 		612.8
(-3)
P 		1090
B 1402.5
(3)
P 		1071.4
(-1.2)
P 		1161.6
(-0)
P 		925.8
(-3)
P 		477
C 1431.6
(0.9)
P 		1363.1
(0.5)
P 		1832
(3)
P 		689.5
(-3)
P 		1143
avr 1512.2
(3) 		1192.6
(0.5) 		1480.9
(2.8) 		742.7
(-3) 		770

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1746
1 2 3 4 5 6 range
A 931.1
(-2.2)
P 		1638.1
(2.3)
P 		1561.5
(1.8)
P 		1745.1
(3)
P 		1650.2
(2.4)
P 		798.6
(-3)
P 		947
B 1826.4
(-2)
P 		1898.9
(-1.5)
P 		2275.4
(1.1)
P 		1830.1
(-2)
P 		2544.2
(3)
P 		1685.1
(-3)
P 		859
C 1662.3
(0.1)
P 		1786.2
(2.4)
P 		1675.4
(0.3)
P 		1532.4
(-2.4)
P 		1820.5
(3)
P 		1498.1
(-3)
P 		322
avr 1473.3
(-1.7) 		1774.4
(1) 		1837.4
(1.5) 		1702.5
(0.3) 		2005
(3) 		1327.3
(-3) 		678
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173276_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27748
Sequence Type Exemplar sequence
Representative Public ID CEC3601 NCBI
Target Description CEC3601_rc /REP_DB=TREMBL Accession /GB=C08360 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk187b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171400(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171400 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-10) (spp-10) mRNA, complete cds. 11/11 A 
SNAP00000012163 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8477165:8478110:1 11/11 None
GENEFINDER00000012181 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8477165:8478110:1 11/11 None
C28C12.7a.2 ENSEMBL cdna:known chromosome:CEL140:IV:8477162:8478146:1 gene:C28C12.7 11/11 None
C28C12.7a.1 ENSEMBL cdna:known chromosome:CEL140:IV:8477164:8478222:1 gene:C28C12.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171399 8/11 Cross Hyb Matching Probes A 
C28C12.7b 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177651 2 1 NM_171399 185955_at (10)
NM_171400 173276_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8477753-8478144(+) 66.01 66.01
Public Domain and Genome References
Ensembl C28C12.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37550 Saposin-like protein family protein 10, isoform a [Caenorhabditis elegans] ref|NP_741466.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 1.0E-134
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 1.8E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 1.7E-9
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 4.7E-11
Sequence
Target Sequence 
>C. ELEGANS:173276_S_AT
ccgtgacttccttcgtgagaacgtgtgcaagagcctcggacagtacagaggattctgcga
tttggtcgttgacgagtatcttccacaattcatccaagagcnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttntgctcaaggactcaa
ggctcgcaaatatgtgggtatcctcggatatttccaacgtcacttccta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTGACTTCCTTCGTGAGAACGTG 666 263 48 Antisense
GACTTCCTTCGTGAGAACGTGTGCA 331 371 52 Antisense
GAACGTGTGCAAGAGCCTCGGACAG 614 347 66 Antisense
GAGCCTCGGACAGTACAGAGGATTC 653 385 78 Antisense
GAGGATTCTGCGATTTGGTCGTTGA 324 403 95 Antisense
ATTTGGTCGTTGACGAGTATCTTCC 672 17 107 Antisense
GAGTATCTTCCACAATTCATCCAAG 494 397 121 Antisense
TGCTCAAGGACTCAAGGCTCGCAAA 256 581 213 Antisense
GGCTCGCAAATATGTGGGTATCCTC 76 539 228 Antisense
GGTATCCTCGGATATTTCCAACGTC 438 483 244 Antisense
CGGATATTTCCAACGTCACTTCCTA 320 243 252 Antisense
Sequence Source Affymetrix Proprietary Database