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[173270_s_at] ubiquinol-cytochrome C reductase

Description CEC650_rc /REP_DB=TREMBL Accession /GB=C07650 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk156b11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 51      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 08      6grp_total > 52      4grp_total > 59      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9365
1 2 3 4 range
A 18637.6
(-0.2)
P 		21402.2
(1.6)
P 		23614
(3)
P 		14249
(-3)
P 		9365
B 20365.8
(2)
P 		20063.4
(1.7)
P 		21394.3
(3)
P 		15404.4
(-3)
P 		5990
C 17139.3
(-0.9)
P 		19332.7
(2.7)
P 		19516.3
(3)
P 		15839.7
(-3)
P 		3677
avr 18714.2
(0.4) 		20266.1
(1.8) 		21508.2
(3) 		15164.4
(-3) 		6344

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7677
1 2 3 4 5 6 range
A 16918.6
(-1.9)
P 		18056.2
(-0.7)
P 		21733.8
(3)
P 		20569.9
(1.8)
P 		16623.6
(-2.2)
P 		15792.2
(-3)
P 		5942
B 17845.1
(-0.2)
P 		18503.1
(0.3)
P 		21860.3
(3)
P 		18140.3
(0.1)
P 		16134.9
(-1.5)
P 		14276.2
(-3)
P 		7584
C 16085
(-2.4)
P 		17943.1
(-0.7)
P 		21953.5
(3)
P 		18495
(-0.2)
P 		16942.5
(-1.6)
P 		15467.6
(-3)
P 		6486
avr 16949.6
(-1.4) 		18167.5
(-0.3) 		21849.2
(3) 		19068.4
(0.5) 		16567
(-1.8) 		15178.7
(-3) 		6671
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4716
1 2 3 4 range
A 6786
(3)
P 		4645.8
(0.3)
P 		6173.2
(2.2)
P 		2085.8
(-3)
P 		4700
B 6494.5
(3)
P 		4955.2
(0.1)
P 		4957.6
(0.1)
P 		3282.7
(-3)
P 		3212
C 5673.6
(1.4)
P 		5558.3
(1.2)
P 		6802
(3)
P 		2670.8
(-3)
P 		4131
avr 6318
(3) 		5053.1
(0.9) 		5977.6
(2.4) 		2679.8
(-3) 		3638

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3437
1 2 3 4 5 6 range
A 3118.8
(-3)
P 		6421.7
(3)
P 		4733.2
(-0.1)
P 		5217
(0.8)
P 		5016.3
(0.4)
P 		3844.9
(-1.7)
P 		3303
B 6209.2
(1.8)
P 		6158.9
(1.6)
P 		5308.3
(-1.3)
P 		4804.7
(-3)
P 		6555.4
(3)
P 		5323.5
(-1.2)
P 		1751
C 5962
(3)
P 		5475
(1.7)
P 		5587.5
(2)
P 		3719.4
(-3)
P 		5482.4
(1.7)
P 		5177.9
(0.9)
P 		2243
avr 5096.7
(-0.8) 		6018.5
(3) 		5209.7
(-0.4) 		4580.4
(-3) 		5684.7
(1.6) 		4782.1
(-2.2) 		1438
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173270_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27655
Sequence Type Exemplar sequence
Representative Public ID CEC650 NCBI
Target Description CEC650_rc /REP_DB=TREMBL Accession /GB=C07650 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk156b11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068960(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068960 NCBI Caenorhabditis elegans Iron-Sulfur Protein family member (isp-1) (isp-1) mRNA, complete cds. 11/11 A 
SNAP00000028278 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8134137:8136021:-1 11/11 None
GENEFINDER00000028290 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8134137:8136021:-1 11/11 None
F42G8.12.2 ENSEMBL cdna:known chromosome:CEL140:IV:8133770:8136038:-1 gene:F42G8.12 11/11 None
F42G8.12.1 ENSEMBL cdna:known chromosome:CEL140:IV:8133770:8136036:-1 gene:F42G8.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8133951-8135019(-) 93.99 93.99
Public Domain and Genome References
Gene Title ubiquinol-cytochrome C reductase
Gene Symbol isp-1
Ensembl F42G8.12
Entrez Gene 177609 Entrez gene
SwissProt O44512 EMBL-EBI
WormBase CE17071 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250235_S_AT ubiquinol-cytochrome C reductase iron-sulfur subunit, mitochondrial, putative / Rieske iron-sulfur protein, putative at
CANINE:1589240_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE:1583675_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE_2:CFA.14355.1.A1_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE_2:CFAAFFX.12289.1.S1_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
DROSOPHILA_2:1641174_AT Rieske iron-sulfur protein dm
DROSOPHILA_2:1631856_A_AT Rieske iron-sulfur protein dm
DROSGENOME1:145592_AT Rieske iron-sulfur protein dm
CHICKEN:GGA.5791.2.S1_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 gga
CHICKEN:GGA.5791.3.S1_S_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 gga
HG-U133_PLUS_2:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-FOCUS:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U133A_2:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U133A:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HUGENEFL:L32977_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
U133_X3P:G12653726_3P_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U95AV2:34401_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U95E:84569_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
MOE430A:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MOUSE430A_2:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MOUSE430_2:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MU11KSUBA:AA445408_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MU19KSUBA:TC19242_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
MU19KSUBA:TC19243_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
MU19KSUBA:TC19244_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
RT-U34:M24542CDS_G_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:M24542CDS_G_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RT-U34:M24542CDS_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:M24542CDS_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:RC_AI103911_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RAE230A:1371254_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RAT230_2:1371254_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
YEAST_2:1775608_AT Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
45285 ubiquinol-cytochrome-c reductase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8121 ubiquinol-cytochrome-c reductase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB92071 Iron-sulfur protein protein 1 [Caenorhabditis elegans] ref|NP_501361.1| Iron-Sulfur Protein family member (isp-1) [Caenorhabditis elegans] 1.0E-151
blast CAE58420 Hypothetical protein CBG01551 [Caenorhabditis briggsae] 1.0E-143
Protein Domains
Database ID Description E-Value
Pfam IPR005806 EMBL-EBI Rieske [2Fe-2S] domain 1.9E-17
Pfam IPR004192 EMBL-EBI Ubiquinol cytochrome reductase transmembrane region 1.4E-42
Sequence
Target Sequence 
>C. ELEGANS:173270_S_AT
ccaattgccgatgctggagattacggaggntattactgcccttgtcacggatctcactcc
gacgcttctggacgtattagaaaggggccagctccattgaatcttcacgtaccagcatac
tcgttcaaggatgccaccatcgtcattggatcgagcta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAATTGCCGATGCTGGAGATTACG 304 277 76 Antisense
TTGCCGATGCTGGAGATTACGGAGG 545 701 80 Antisense
CGACGCTTCTGGACGTATTAGAAAG 130 251 135 Antisense
CCAGCTCCATTGAATCTTCACGTAC 279 273 163 Antisense
TGAATCTTCACGTACCAGCATACTC 673 575 173 Antisense
TTCACGTACCAGCATACTCGTTCAA 404 683 179 Antisense
GTACCAGCATACTCGTTCAAGGATG 132 457 184 Antisense
ATACTCGTTCAAGGATGCCACCATC 378 21 192 Antisense
GTTCAAGGATGCCACCATCGTCATT 660 443 198 Antisense
GATGCCACCATCGTCATTGGATCGA 213 415 205 Antisense
CCACCATCGTCATTGGATCGAGCTA 627 273 209 Antisense
Sequence Source Affymetrix Proprietary Database