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[173258_s_at] PH domain

Description g561675 /REP_DB=GenBank Identifier /FEA=mRNA Transcript /GEN=cesema /DEF=CeSema
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 52      4grp_highest_8_8 > 60      4grp_high_8_8 > 17      6grp_highest_nonorm > 72      6grp_total > 62      4grp_total > 31      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 446.2
(3)
P 		353.2
(-2.2)
P 		339.2
(-3)
P 		354.4
(-2.1)
P 		107
B 404.7
(3)
P 		359.3
(1)
P 		265.2
(-3)
P 		347.7
(0.5)
P 		140
C 441.6
(3)
P 		301.4
(-3)
P 		320.1
(-2.2)
P 		403.6
(1.4)
P 		140
avr 430.8
(3) 		338
(-1.5) 		308.2
(-3) 		368.6
(-0) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 308
1 2 3 4 5 6 range
A 280.7
(-3)
P 		523.3
(2.4)
P 		354.9
(-1.4)
P 		552.6
(3)
P 		288.6
(-2.8)
P 		472.9
(1.2)
P 		272
B 302
(-2.7)
P 		366.4
(-0.8)
P 		495.2
(3)
P 		301.7
(-2.7)
P 		477.4
(2.5)
P 		293
(-3)
P 		202
C 407.9
(-0.8)
P 		452.9
(0.1)
P 		588.2
(3)
P 		417.8
(-0.6)
P 		411.5
(-0.8)
P 		305.8
(-3)
P 		282
avr 330.2
(-3) 		447.5
(1.7) 		479.4
(3) 		424
(0.8) 		392.5
(-0.5) 		357.2
(-1.9) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 162.5
(3)
P 		76.7
(-1.7)
P 		88.7
(-1)
P 		51.9
(-3)
P 		111
B 129.1
(3)
P 		88.7
(-0.6)
P 		61.4
(-3)
P 		74.1
(-1.9)
P 		68
C 146.2
(3)
P 		86.7
(-1.6)
P 		111.6
(0.3)
P 		68
(-3)
P 		78
avr 145.9
(3) 		84
(-1.6) 		87.2
(-1.3) 		64.7
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 51.7
(-3)
P 		186.1
(3)
P 		77.3
(-1.9)
P 		140.2
(1)
P 		87.1
(-1.4)
P 		115.1
(-0.2)
P 		134
B 105.1
(-1.7)
P 		122
(-0.8)
P 		120.3
(-0.9)
P 		79.9
(-3)
P 		193.9
(3)
P 		109.2
(-1.5)
P 		114
C 151.2
(3)
P 		138.2
(1.8)
P 		149.7
(2.9)
P 		84
(-3)
P 		133.2
(1.4)
P 		102.4
(-1.4)
P 		67
avr 102.7
(-2.8) 		148.8
(3) 		115.8
(-1.2) 		101.4
(-3) 		138.1
(1.6) 		108.9
(-2.1) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173258_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28080
Sequence Type Exemplar sequence
Representative Public ID 561675 NCBI
Target Description g561675 /REP_DB=GenBank Identifier /FEA=mRNA Transcript /GEN=cesema /DEF=CeSema
Cluster Evidence This cluster is supported by a mRNA.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060361(11), U15667(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060361 NCBI Caenorhabditis elegans F25H2.6 (F25H2.6) mRNA, complete cds. 11/11 A 
U15667 NCBI Caenorhabditis elegans semaphorin CeSema (cesema) mRNA, complete cds. 11/11 A 
GENEFINDER00000042639 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10562372:10565752:1 11/11 None
SNAP00000042619 ENSEMBL cdna:SNAP chromosome:CEL140:I:10562758:10565752:1 11/11 None
F25H2.6.3 ENSEMBL cdna:known chromosome:CEL140:I:10562485:10565956:1 gene:F25H2.6 11/11 None
F25H2.6.2 ENSEMBL cdna:known chromosome:CEL140:I:10562487:10565956:1 gene:F25H2.6 11/11 A 
F25H2.6.1 ENSEMBL cdna:known chromosome:CEL140:I:10562589:10565956:1 gene:F25H2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14815515-14821878(+) 83.19 83.19
Public Domain and Genome References
Gene Title PH domain
Ensembl F25H2.6
Entrez Gene 172940 Entrez gene
SwissProt Q93569 EMBL-EBI
WormBase CE23681 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1601014_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
CANINE_2:CFA.18065.1.S1_S_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
CANINE_2:CFA.18065.2.A1_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
CANINE_2:CFAAFFX.14920.1.S1_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
CANINE_2:CFAAFFX.14920.1.S1_S_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
CANINE_2:CFAAFFX.14929.1.S1_S_AT similar to Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein) (StAR-related lipid transfer protein 11) (StARD11) (START domain-containing protein 11) cfa
DROSOPHILA_2:1626968_AT dm
DROSGENOME1:152129_AT dm
CHICKEN:GGA.10791.1.S1_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein gga
CHICKEN:GGAAFFX.24551.1.S1_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein gga
HG-U133_PLUS_2:219625_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133A:219625_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-FOCUS:219625_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133A_2:219625_S_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133_PLUS_2:223466_X_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133B:223466_X_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HU35KSUBB:RC_AA040087_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
U133_X3P:G12652706_3P_A_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
U133_X3P:HS.21276.1.S2_3P_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
U133_X3P:HS.21276.1.S2_3P_A_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133_PLUS_2:223465_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133B:223465_AT collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HU35KSUBA:RC_AA291710_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HU35KSUBD:RC_D60624_S_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HU35KSUBD:RC_AA461090_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
U133_X3P:HS.21276.2.S1_3P_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133_PLUS_2:226277_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133B:226277_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U95C:49009_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133_PLUS_2:238279_X_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U133B:238279_X_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U95D:81430_R_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HG-U95D:88114_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
HU35KSUBC:RC_AA282568_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
U133_X3P:HS.270437.0.A1_3P_AT Collagen, type IV, alpha 3 (Goodpasture antigen) binding protein hs
MG-U74BV2:112739_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MG-U74BV2:105660_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MG-U74BV2:116593_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MG-U74BV2:106303_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOE430A:1420383_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430A_2:1420383_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430_2:1420383_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOE430A:1449847_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430A_2:1449847_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430_2:1449847_A_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOE430A:1420384_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430A_2:1420384_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430_2:1420384_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOE430B:1452867_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MOUSE430_2:1452867_AT procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein mm
MU19KSUBB:TC29427_AT Procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein, mRNA (cDNA clone MGC:27595 IMAGE:4502118) mm
MU19KSUBC:TC40886_AT Procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein, mRNA (cDNA clone MGC:27595 IMAGE:4502118) mm
RAE230B:1394775_AT Procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein (predicted) rn
RAT230_2:1394775_AT Procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB02094 Hypothetical protein F25H2.6 [Caenorhabditis elegans] ref|NP_492762.1| F25H2.6 [Caenorhabditis elegans] 0.0
blast CAE58975 Hypothetical protein CBG02248 [Caenorhabditis briggsae] 0.0
blast AAA51021 CeSema 0.0
blast CAA21714 Hypothetical protein Y54E5B.1a [Caenorhabditis elegans] ref|NP_493582.3| SeMaPhorin related family member (smp-1) [Caenorhabditis elegans] sp|Q17330|SEM1A_CAEEL Semaphorin 1A precursor (CeSema) 0.0
blast CAE72526 Hypothetical protein CBG19706 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 8.6E-17
Pfam IPR002913 EMBL-EBI Lipid-binding START 9.9E-65
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.0E-126
Pfam IPR002165 EMBL-EBI Plexin 1.3E-5
Pfam IPR001849 EMBL-EBI Pleckstrin-like 8.6E-17
Pfam IPR002913 EMBL-EBI Lipid-binding START 9.9E-65
Sequence
Target Sequence 
>C. ELEGANS:173258_S_AT
gattgtgatgagttctttgacgccgacgagtttgatgtggactcgaaaaatggagatacc
gttgaaaaaagacaagaagaggtggaagacaagaaaaatggaaatatagttgttccagag
gaagagaaaaagaagactagcttcagaattgaaggaaacccagttactggtcactatgac
gacctcctcgtgagccaagaacactctctttacaccacaatcgataagctcgctctagaa
cagctcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGTGATGAGTTCTTTGACGCCG 557 435 1615 Antisense
AGTTCTTTGACGCCGACGAGTTTGA 675 59 1625 Antisense
TTTGACGCCGACGAGTTTGATGTGG 186 661 1630 Antisense
AGTTTGATGTGGACTCGAAAAATGG 434 61 1643 Antisense
ATGGAGATACCGTTGAAAAAAGACA 35 51 1664 Antisense
GAAGGAAACCCAGTTACTGGTCACT 658 329 1765 Antisense
TACTGGTCACTATGACGACCTCCTC 165 645 1779 Antisense
GAGCCAAGAACACTCTCTTTACACC 247 385 1806 Antisense
ACTCTCTTTACACCACAATCGATAA 191 101 1817 Antisense
ACACCACAATCGATAAGCTCGCTCT 375 109 1826 Antisense
ATAAGCTCGCTCTAGAACAGCTCAA 410 25 1838 Antisense
Sequence Source GenBank