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[173242_s_at]

Description CEC0235 /REP_DB=TREMBL Accession /GB=C13023 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk162h11 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 10      6grp_total > 17      4grp_total > 04      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2959
1 2 3 4 range
A 1217.6
(-1.4)
P 		653.1
(-2.6)
P 		458.9
(-3)
P 		3230.7
(3)
P 		2772
B 1306.4
(-1.4)
P 		632.2
(-2.8)
P 		542.6
(-3)
P 		3351.5
(3)
P 		2809
C 1102
(-1.4)
P 		637
(-2.5)
P 		392.3
(-3)
P 		3088.2
(3)
P 		2696
avr 1208.7
(-1.4) 		640.8
(-2.6) 		464.6
(-3) 		3223.5
(3) 		2759

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 813
1 2 3 4 5 6 range
A 1853.8
(3)
P 		1235.8
(-3)
P 		1452.5
(-0.9)
P 		1260.7
(-2.8)
P 		1261.6
(-2.7)
P 		1329.8
(-2.1)
P 		618
B 1616.9
(3)
P 		1342.9
(-0.7)
P 		1286.6
(-1.5)
P 		1259.5
(-1.8)
P 		1234.7
(-2.2)
P 		1174.2
(-3)
P 		443
C 1709
(3)
P 		1312.5
(-0.6)
P 		1208.7
(-1.5)
P 		1041
(-3)
P 		1269.9
(-0.9)
P 		1258.4
(-1)
P 		668
avr 1726.6
(3) 		1297.1
(-1.8) 		1315.9
(-1.6) 		1187.1
(-3) 		1255.4
(-2.2) 		1254.1
(-2.3) 		540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 594
1 2 3 4 range
A 443.3
(2.5)
P 		141.8
(-2.6)
P 		120
(-3)
P 		472.9
(3)
P 		353
B 416.6
(-0)
P 		156.1
(-2.7)
P 		125.7
(-3)
P 		714.2
(3)
P 		589
C 364.8
(0.6)
P 		183.1
(-2.3)
P 		136.7
(-3)
P 		520.7
(3)
P 		384
avr 408.2
(0.8) 		160.3
(-2.6) 		127.5
(-3) 		569.3
(3) 		442

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 341.7
(-1.8)
P 		439.5
(3)
P 		316.3
(-3)
P 		319.7
(-2.8)
P 		380.7
(0.1)
P 		323.8
(-2.6)
P 		123
B 562.6
(3)
P 		447
(0.2)
P 		312.4
(-3)
P 		333.6
(-2.5)
P 		501.6
(1.5)
P 		437.9
(0)
P 		250
C 633.5
(3)
P 		400.5
(-0.3)
P 		307.6
(-1.6)
P 		209.3
(-3)
P 		410.9
(-0.1)
P 		421.3
(-0)
P 		424
avr 512.6
(3) 		429
(0.8) 		312.1
(-2.3) 		287.5
(-3) 		431.1
(0.8) 		394.3
(-0.2) 		225
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173242_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27729
Sequence Type Exemplar sequence
Representative Public ID CEC0235 NCBI
Target Description CEC0235 /REP_DB=TREMBL Accession /GB=C13023 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk162h11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058507(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058507 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-12) (nlp-12) mRNA, complete cds. 11/11 None
SNAP00000009589 ENSEMBL cdna:SNAP chromosome:CEL140:I:1843408:1843786:1 11/11 None
GENEFINDER00000009605 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1843408:1843786:1 11/11 None
M01D7.5 ENSEMBL cdna:known chromosome:CEL140:I:1843401:1843903:1 gene:M01D7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1843401-1843903(+) 96.32 96.32
Public Domain and Genome References
Gene Title
Gene Symbol nlp-12
Ensembl M01D7.5
Entrez Gene 171753 Entrez gene
SwissProt O01970 EMBL-EBI
WormBase CE12268 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB58066 Neuropeptide-like protein protein 12 [Caenorhabditis elegans] ref|NP_490908.1| Neuropeptide-Like Protein family member (nlp-12) [Caenorhabditis elegans] 5.0E-42
blast CAE74463 Hypothetical protein CBG22209 [Caenorhabditis briggsae] 1.0E-33
blast AAQ23186 neuropeptide-like protein NLP-12 precursor [Ascaris suum] 2.0E-16
Sequence
Target Sequence 
>C. ELEGANS:173242_S_AT
gcaacacagtcgccgacgtttgaccgtcaggatcgcgattaccggccacttcagttcggc
aagcgagatgggtaccgtccattgcagttcggaaaacgcgactatcggcctctccagttc
ggaaagcgaagcagcggcagctctggaccagttgtgctcgagccaatctgggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACACAGTCGCCGACGTTTGACC 514 319 81 Antisense
GCCGACGTTTGACCGTCAGGATCGC 497 283 92 Antisense
ACCGTCAGGATCGCGATTACCGGCC 572 81 103 Antisense
GCCACTTCAGTTCGGCAAGCGAGAT 468 279 125 Antisense
AAGCGAGATGGGTACCGTCCATTGC 103 145 141 Antisense
GGTACCGTCCATTGCAGTTCGGAAA 637 503 151 Antisense
TTCGGAAAACGCGACTATCGGCCTC 326 693 168 Antisense
GGCCTCTCCAGTTCGGAAAGCGAAG 428 543 187 Antisense
CGGAAAGCGAAGCAGCGGCAGCTCT 632 241 200 Antisense
CGGCAGCTCTGGACCAGTTGTGCTC 299 243 215 Antisense
AGTTGTGCTCGAGCCAATCTGGGAA 297 65 230 Antisense
Sequence Source Affymetrix Proprietary Database