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[173226_s_at]

Description g1619862 /REP_DB=GenBank Identifier /FEA=mRNA Transcript
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 61      6grp_total > 05      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 202
1 2 3 4 range
A 585.4
(3)
P 		476.6
(-0.7)
P 		409.2
(-3)
P 		500.2
(0.1)
P 		176
B 604.9
(3)
P 		481.7
(-0.7)
P 		443.7
(-1.8)
P 		402.5
(-3)
P 		202
C 499.7
(-3)
P 		521.8
(-1.5)
P 		499.8
(-3)
P 		590.1
(3)
P 		90
avr 563.3
(3) 		493.4
(-0.7) 		450.9
(-3) 		497.6
(-0.5) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 449.8
(0.5)
P 		549.9
(3)
P 		370.7
(-1.6)
P 		371.2
(-1.6)
P 		374.9
(-1.5)
P 		314.3
(-3)
P 		236
B 512.2
(3)
P 		454
(1.5)
P 		400.3
(0.2)
P 		346
(-1.2)
P 		508.2
(2.9)
P 		275
(-3)
P 		237
C 682
(3)
P 		456.1
(-0.5)
P 		424.7
(-0.9)
P 		340.7
(-2.2)
P 		395.7
(-1.4)
P 		289.6
(-3)
P 		392
avr 548
(3) 		486.7
(1.6) 		398.6
(-0.5) 		352.6
(-1.6) 		426.3
(0.1) 		293
(-3) 		255
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 213.1
(3)
P 		103.5
(-1.7)
P 		107
(-1.6)
P 		73.2
(-3)
P 		140
B 192.9
(3)
P 		119
(-1.1)
P 		102.8
(-2)
P 		85.8
(-3)
P 		107
C 165.4
(2.3)
P 		150
(1.1)
P 		174.2
(3)
P 		99.5
(-3)
P 		75
avr 190.5
(3) 		124.2
(-0.8) 		128
(-0.6) 		86.2
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 82.9
(-2.7)
P 		195.6
(3)
P 		80.7
(-2.8)
P 		94.1
(-2.1)
P 		113.1
(-1.2)
P 		76.5
(-3)
P 		119
B 178.2
(1.5)
P 		151.1
(0.1)
P 		97.2
(-2.7)
P 		91.7
(-3)
P 		206.5
(3)
P 		102.6
(-2.4)
P 		115
C 252.8
(3)
P 		139.2
(-0.7)
P 		108.1
(-1.7)
P 		68.5
(-3)
P 		128
(-1.1)
P 		96.9
(-2.1)
P 		184
avr 171.3
(3) 		162
(2.4) 		95.3
(-2.3) 		84.8
(-3) 		149.2
(1.5) 		92
(-2.5) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173226_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28100
Sequence Type Exemplar sequence
Representative Public ID 1619862 NCBI
Target Description g1619862 /REP_DB=GenBank Identifier /FEA=mRNA Transcript
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060975(11), U68262(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060975 NCBI Caenorhabditis elegans RAB family member (rab-27) (rab-27) mRNA, complete cds. 11/11 A 
U68262 NCBI Caenorhabditis elegans RAM1-like mRNA, partial cds. 11/11 None
GENEFINDER00000019615 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13551507:13553303:-1 11/11 None
Y87G2A.4 ENSEMBL cdna:known chromosome:CEL140:I:13551213:13553307:-1 gene:Y87G2A.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019586 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13552971-13553247(-) 89.52 89.52
Public Domain and Genome References
Gene Title
Gene Symbol rab-27
Ensembl Y87G2A.4
Entrez Gene 173223 Entrez gene
SwissProt Q9NA29 EMBL-EBI
WormBase CE23130 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.24390.1.S1_AT similar to Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram p25) cfa
DROSOPHILA_2:1629621_A_AT dm
DROSGENOME1:153277_AT dm
CHICKEN:GGAAFFX.2750.1.S1_S_AT similar to Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram p25) gga
HG-U133_PLUS_2:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U133A:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U95AV2:809_AT RAB27A, member RAS oncogene family hs
HC-G110:809_AT RAB27A, member RAS oncogene family hs
HG-U133A:209515_S_AT RAB27A, member RAS oncogene family hs
HG-U133_PLUS_2:209515_S_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:209515_S_AT RAB27A, member RAS oncogene family hs
HU35KSUBC:RC_AA278355_AT RAB27A, member RAS oncogene family hs
HU35KSUBD:RC_N73083_AT RAB27A, member RAS oncogene family hs
HUGENEFL:U57094_AT RAB27A, member RAS oncogene family hs
U133_X3P:G4887230_3P_A_AT RAB27A, member RAS oncogene family hs
U133_X3P:HS.50477.1.S1_3P_A_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:209514_S_AT RAB27A, member RAS oncogene family hs
HG-FOCUS:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U133_PLUS_2:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U133A:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U95AV2:40669_AT RAB27A, member RAS oncogene family hs
HG-U95C:65080_R_AT RAB27A, member RAS oncogene family hs
HG-U95E:68324_AT RAB27A, member RAS oncogene family hs
HG-U95E:86890_AT RAB27A, member RAS oncogene family hs
HG-U95E:89586_S_AT RAB27A, member RAS oncogene family hs
MOUSE430_2:1425284_A_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1425284_A_AT RAB27A, member RAS oncogene family mm
MOE430A:1425284_A_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1425285_A_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1425285_A_AT RAB27A, member RAS oncogene family mm
MOE430A:1425285_A_AT RAB27A, member RAS oncogene family mm
MG-U74BV2:163478_AT RAB27A, member RAS oncogene family mm
MOE430B:1429123_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1429123_AT RAB27A, member RAS oncogene family mm
MOE430A:1454438_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1454438_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1454438_AT RAB27A, member RAS oncogene family mm
MU19KSUBC:TC41274_AT RAB27A, member RAS oncogene family, mRNA (cDNA clone MGC:6415 IMAGE:3590089) mm
RAE230A:1387763_AT low Mr GTP-binding protein rn
RAT230_2:1387763_AT low Mr GTP-binding protein rn
RG-U34A:D17352_AT low Mr GTP-binding protein rn
RG-U34B:RC_AI043615_AT low Mr GTP-binding protein rn
RG-U34C:RC_AI237416_AT low Mr GTP-binding protein rn
RAE230B:1377669_AT low Mr GTP-binding protein rn
RAT230_2:1377669_AT low Mr GTP-binding protein rn
RAE230B:1384852_AT low Mr GTP-binding protein rn
RAT230_2:1384852_AT low Mr GTP-binding protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54484 Hypothetical protein Y87G2A.4 [Caenorhabditis elegans] ref|NP_493376.1| RAB family member (rab-27) [Caenorhabditis elegans] dbj|BAD07035.1| Rab27 [Caenorhabditis elegans] 1.0E-113
blast CAE63548 Hypothetical protein CBG08034 [Caenorhabditis briggsae] 1.0E-109
blast AAH82420 LOC494641 protein [Xenopus laevis] 2.0E-70
blast AAB16977 RAM1-like [Caenorhabditis elegans] 5.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.7E-85
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.7E-25
Sequence
Target Sequence 
>C. ELEGANS:173226_S_AT
atgggtgactacgactatctcatcaaatttctcgcgcccggcgatccgggagtcggaaaa
acgtcgtttttgcatcgttacacggataacacgttcaccggacaattcatttccactgtt
ggaattgattttaaagagaaaaaagtggtctacgagagttcacgtggcggattcggtggt
cgtggtcaacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGGTGACTACGACTATCTCATCA 327 49 39 Antisense
GACTACGACTATCTCATCAAATTTC 44 373 45 Antisense
CGACTATCTCATCAAATTTCTCGCG 705 251 50 Antisense
ACGTCGTTTTTGCATCGTTACACGG 301 95 99 Antisense
GTTACACGGATAACACGTTCACCGG 617 443 115 Antisense
GGATAACACGTTCACCGGACAATTC 47 509 122 Antisense
ACACGTTCACCGGACAATTCATTTC 78 109 127 Antisense
CACCGGACAATTCATTTCCACTGTT 243 201 134 Antisense
CGGACAATTCATTTCCACTGTTGGA 436 243 137 Antisense
TCCACTGTTGGAATTGATTTTAAAG 25 589 150 Antisense
CGGATTCGGTGGTCGTGGTCAACGA 314 243 206 Antisense
Sequence Source GenBank