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[173219_s_at]

Description AV182704_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV182704 /DEF=Caenorhabditis elegans cDNA clone:yk641e10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 11      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 60      6grp_total > 13      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 377
1 2 3 4 range
A 482.4
(3)
P 		235.6
(-2.2)
P 		197.7
(-3)
P 		216
(-2.6)
P 		285
B 542
(3)
P 		241.8
(-2.1)
P 		188.7
(-3)
P 		188.5
(-3)
P 		354
C 544.9
(3)
P 		168.3
(-3)
P 		190
(-2.7)
P 		200.9
(-2.5)
P 		377
avr 523.1
(3) 		215.2
(-2.6) 		192.1
(-3) 		201.8
(-2.8) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 283
1 2 3 4 5 6 range
A 475.4
(3)
P 		392
(1.1)
P 		408.1
(1.5)
P 		407.7
(1.5)
P 		282
(-1.4)
P 		211
(-3)
P 		264
B 460.7
(3)
P 		413.9
(1.8)
P 		373.8
(0.8)
P 		377.1
(0.9)
P 		308.7
(-0.9)
P 		226.2
(-3)
P 		235
C 358.8
(0.1)
P 		494.3
(3)
P 		420.6
(1.4)
P 		341.8
(-0.3)
P 		234.3
(-2.6)
P 		218.1
(-3)
P 		276
avr 431.6
(2.9) 		433.4
(3) 		400.8
(2.1) 		375.5
(1.4) 		275
(-1.4) 		218.4
(-3) 		215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 175.6
(3)
P 		51.1
(-2.2)
P 		51.7
(-2.2)
P 		31.6
(-3)
P 		144
B 172.8
(3)
P 		59.7
(-2.1)
P 		43.7
(-2.8)
P 		40.2
(-3)
P 		133
C 180.4
(3)
P 		48.4
(-2.4)
P 		66.2
(-1.7)
P 		33.9
(-3)
P 		147
avr 176.3
(3) 		53.1
(-2.2) 		53.9
(-2.2) 		35.2
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 87.6
(-0.5)
P 		139.4
(3)
P 		88.9
(-0.4)
P 		103.4
(0.5)
P 		85.1
(-0.7)
P 		51.4
(-3)
P 		88
B 160.3
(3)
P 		137.8
(1.2)
P 		90.8
(-2.5)
P 		99.9
(-1.8)
P 		125.4
(0.2)
P 		84.4
(-3)
P 		76
C 133
(1.7)
P 		150.8
(3)
P 		107
(-0.2)
P 		68.7
(-3)
P 		75.8
(-2.5)
P 		73
(-2.7)
P 		82
avr 127
(1.7) 		142.7
(3) 		95.6
(-0.9) 		90.7
(-1.3) 		95.4
(-0.9) 		69.6
(-3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173219_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26818
Sequence Type Exemplar sequence
Representative Public ID AV182704 NCBI
Target Description AV182704_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV182704 /DEF=Caenorhabditis elegans cDNA clone:yk641e10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066089(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066089 NCBI Caenorhabditis elegans mammalian ZAK kinase homolog family member (zak-1) (zak-1) mRNA, complete cds. 11/11 A 
R13F6.6a ENSEMBL cdna:known chromosome:CEL140:III:6853060:6856715:-1 gene:R13F6.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_181912 7/11 Cross Hyb Matching Probes A 
SNAP00000003260 7/11 Cross Hyb Matching Probes None
GENEFINDER00000003271 7/11 Cross Hyb Matching Probes None
R13F6.6b.1 7/11 Cross Hyb Matching Probes None
R13F6.6b.2 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175954 2 1 NM_066089 173219_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6853154-6853455(-) 84.33 84.33
Public Domain and Genome References
Ensembl R13F6.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498490 mammalian ZAK kinase homolog family member (zak-1) [Caenorhabditis elegans] gb|AAC47047.4| Mammalian zak kinase homolog protein 1, isoform a [Caenorhabditis elegans] 0.0
blast CAE70100 Hypothetical protein CBG16544 [Caenorhabditis briggsae] 0.0
blast NP_871641 mammalian ZAK kinase homolog family member (zak-1) [Caenorhabditis elegans] gb|AAN65305.1| Mammalian zak kinase homolog protein 1, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.5E-17
Sequence
Target Sequence 
>C. ELEGANS:173219_S_AT
tccgacacaaataataagacgcctgaatctcaagcacgccgagttcatgtacacggtgga
aaggataaatggaactggaaaaagggaaaatctcggcccaaatttacctgattcagtaag
aaaagaatttgaagtttgaacgggttcaacactcatcaattagatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGACACAAATAATAAGACGCCTG 584 593 78 Antisense
TAAGACGCCTGAATCTCAAGCACGC 46 637 92 Antisense
GAATCTCAAGCACGCCGAGTTCATG 513 327 102 Antisense
CAAGCACGCCGAGTTCATGTACACG 386 185 108 Antisense
GCACGCCGAGTTCATGTACACGGTG 74 315 111 Antisense
GCCGAGTTCATGTACACGGTGGAAA 389 285 115 Antisense
GAAAATCTCGGCCCAAATTTACCTG 231 351 163 Antisense
ATCTCGGCCCAAATTTACCTGATTC 541 31 167 Antisense
TCGGCCCAAATTTACCTGATTCAGT 159 607 170 Antisense
TGAACGGGTTCAACACTCATCAATT 307 573 214 Antisense
GGGTTCAACACTCATCAATTAGATT 80 497 219 Antisense
Sequence Source Affymetrix Proprietary Database