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[173211_at] E1-E2 ATPase

Description AV179768_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV179768 /DEF=Caenorhabditis elegans cDNA clone:yk597g11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 55      6grp_total > 70      4grp_total > 60      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 906
1 2 3 4 range
A 1105.8
(0.3)
P 		1116.6
(0.4)
P 		1345.1
(3)
P 		809.7
(-3)
P 		535
B 1520.1
(0.8)
P 		1716.1
(3)
P 		1479.7
(0.4)
P 		1175.8
(-3)
P 		540
C 1315.2
(3)
P 		1078.9
(-0.8)
P 		1313.1
(3)
P 		942.1
(-3)
P 		373
avr 1313.7
(2) 		1303.9
(1.9) 		1379.3
(3) 		975.9
(-3) 		403

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1411
1 2 3 4 5 6 range
A 1598.5
(0.7)
P 		1814.6
(3)
P 		1256.4
(-3)
P 		1682.8
(1.6)
P 		1748.2
(2.3)
P 		1598
(0.7)
P 		558
B 1640.8
(0.7)
P 		1823.7
(1.9)
P 		1983.5
(3)
P 		1967.9
(2.9)
P 		1285
(-1.7)
P 		1095.1
(-3)
P 		888
C 1465
(-2.4)
P 		1632.8
(-1.5)
P 		1561.9
(-1.9)
P 		2506.1
(3)
P 		1344.5
(-3)
P 		1545.8
(-2)
P 		1162
avr 1568.1
(-1.5) 		1757
(0.2) 		1600.6
(-1.2) 		2052.3
(3) 		1459.2
(-2.6) 		1413
(-3) 		639
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 366
1 2 3 4 range
A 402.6
(3)
P 		242.4
(-0.4)
P 		351.6
(1.9)
P 		118.5
(-3)
P 		284
B 484.7
(3)
P 		423.8
(1.4)
P 		342.9
(-0.6)
P 		250.6
(-3)
P 		234
C 435.4
(2.6)
P 		310.2
(0)
P 		457.6
(3)
P 		158.9
(-3)
P 		299
avr 440.9
(3) 		325.5
(0.4) 		384
(1.7) 		176
(-3) 		265

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 372
1 2 3 4 5 6 range
A 294.7
(-2.7)
P 		645.4
(3)
P 		273.6
(-3)
P 		426.8
(-0.5)
P 		527.5
(1.1)
P 		389.1
(-1.1)
P 		372
B 570.9
(1.9)
P 		607
(3)
P 		481.6
(-0.8)
P 		521.2
(0.4)
P 		522.1
(0.4)
P 		408.3
(-3)
P 		199
C 543
(3)
P 		498.2
(1.2)
P 		397.5
(-3)
P 		504
(1.4)
P 		435
(-1.5)
P 		517.5
(1.9)
P 		146
avr 469.5
(-0.4) 		583.5
(3) 		384.2
(-3) 		484
(0) 		494.9
(0.3) 		438.3
(-1.4) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173211_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26674
Sequence Type Exemplar sequence
Representative Public ID AV179768 NCBI
Target Description AV179768_rc /REP_DB=TREMBL Accession /FEA=EST Transcript /REVCOMP /GB=AV179768 /DEF=Caenorhabditis elegans cDNA clone:yk597g11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a EST Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069764(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069764 NCBI Caenorhabditis elegans C10C6.6 (C10C6.6) mRNA, complete cds. 11/11 None
C10C6.6 ENSEMBL cdna:known chromosome:CEL140:IV:11473338:11479222:-1 gene:C10C6.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11473433-11473703(-) 90.0 90.0
Public Domain and Genome References
Gene Title E1-E2 ATPase
Ensembl C10C6.6
Entrez Gene 178070 Entrez gene
SwissProt P90747 EMBL-EBI
WormBase CE30858 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249846_AT ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase familiy protein at
CANINE:1585023_AT similar to Probable cation-transporting ATPase 13A1 cfa
CANINE_2:CFA.7172.1.A1_AT similar to Probable cation-transporting ATPase 13A1 cfa
CANINE_2:CFAAFFX.21853.1.S1_AT similar to Probable cation-transporting ATPase 13A1 cfa
CANINE_2:CFAAFFX.21853.1.S1_S_AT similar to Probable cation-transporting ATPase 13A1 cfa
DROSOPHILA_2:1626930_AT dm
DROSGENOME1:152048_AT dm
CHICKEN:GGAAFFX.24199.1.S1_AT similar to Probable cation-transporting ATPase 13A (CGI-152) gga
CHICKEN:GGAAFFX.24199.1.S1_S_AT similar to Probable cation-transporting ATPase 13A (CGI-152) gga
HG-U133A:218052_S_AT ATPase type 13A1 hs
HG-U133_PLUS_2:218052_S_AT ATPase type 13A1 hs
HG-U133A_2:218052_S_AT ATPase type 13A1 hs
HU35KSUBA:RC_AA398522_AT ATPase type 13A1 hs
U133_X3P:G9966896_3P_S_AT ATPase type 13A1 hs
HG-U95B:46692_AT ATPase type 13A1 hs
MU11KSUBA:AA184119_S_AT ATPase type 13A1 mm
MG-U74AV2:95029_AT ATPase type 13A1 mm
MOE430A:1417475_AT ATPase type 13A1 mm
MOUSE430A_2:1417475_AT ATPase type 13A1 mm
MOUSE430_2:1417475_AT ATPase type 13A1 mm
RG-U34A:U21720MRNA_AT ATPase type 13A (predicted) rn
RG-U34A:RC_AA893621_AT ATPase type 13A (predicted) rn
RAE230A:1371925_AT ATPase type 13A (predicted) rn
RAT230_2:1371925_AT ATPase type 13A (predicted) rn
YEAST_2:1769375_AT P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
16887 ATPase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05683 Hypothetical protein C10C6.6 [Caenorhabditis elegans] ref|NP_502165.2| C10C6.6 [Caenorhabditis elegans] sp|P90747|YE56_CAEEL Probable cation-transporting ATPase C10C6.6 in chromosome IV 0.0
blast T19187 hypothetical protein C10C6.6 - Caenorhabditis elegans 0.0
blast CAE62112 Hypothetical protein CBG06150 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A8A1_HUMAN (Q9Y2Q0) Potential phospholipid-transporting ATPase IA (EC 3.6.3.1) (Chromaffin granule ATPase II) (ATPase class I type 8A member 1) 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.1E-15
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 8.3E-5
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 6.3E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502165.2 9 20-42,52-74,186-208,212-230,395-414,996-1013,1034-1056,1076-1098,1111-1133
C10C6.6 9 20-42,52-74,186-208,212-230,395-414,996-1013,1034-1056,1076-1098,1111-1133
Sequence
Target Sequence 
>C. ELEGANS:173211_AT
tatgttctaaattactcgtttttcttattatccttcttccttgacattccctatcaacat
cttttttctgccatcctgttgtttaatttattttttcggttttatcttcacttagaacca
aagctatcgcatgatctaagatcagaaatagtcatttcattgtcgctgtttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGTTCTAAATTACTCGTTTTTCT 671 651 97 Antisense
TTCCTTGACATTCCCTATCAACATC 665 699 133 Antisense
ATTCCCTATCAACATCTTTTTTCTG 354 9 142 Antisense
CTATCAACATCTTTTTTCTGCCATC 178 225 147 Antisense
TTTTTCTGCCATCCTGTTGTTTAAT 345 675 159 Antisense
CTGCCATCCTGTTGTTTAATTTATT 213 237 164 Antisense
TCGGTTTTATCTTCACTTAGAACCA 549 605 192 Antisense
ATCTTCACTTAGAACCAAAGCTATC 531 29 200 Antisense
GAACCAAAGCTATCGCATGATCTAA 407 345 211 Antisense
AAAGCTATCGCATGATCTAAGATCA 205 125 216 Antisense
ATAGTCATTTCATTGTCGCTGTTTC 164 23 244 Antisense
Sequence Source Affymetrix Proprietary Database